A5017213734- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Cell Image Analysis Techniques
- Lipid Membrane Structure and Behavior
- Biometric Identification and Security
- Single-cell and spatial transcriptomics
- Spectroscopy and Quantum Chemical Studies
- Microfluidic and Bio-sensing Technologies
- Image and Object Detection Techniques
- Spaceflight effects on biology
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Nanopore and Nanochannel Transport Studies
- Planarian Biology and Electrostimulation
- thermodynamics and calorimetric analyses
- Cancer Research and Treatments
- Mathematical Biology Tumor Growth
- Erythrocyte Function and Pathophysiology
Ben-Gurion University of the Negev
2021-2024
Harvard University
2016-2019
Technion – Israel Institute of Technology
2012-2014
Google (United States)
2014
Recent experimental studies provide evidence for the existence of a spatially non-uniform temperature field in living cells and particular their plasma membrane. These findings have led to development new exciting field: thermal biology at single-cell level. Here, we examine theoretically specific aspect this field, i.e. how gradients level affect phase behaviour geometry heterogeneous membranes. We address issue by using Onsager reciprocal relations combined with simple model binary lipid...
Loss of function the tumor suppressor p53 is known to increase rate migration cells transiting narrow pores traditional Boyden chamber assay. Here by contrast we investigate how impacts cellular within a 2D confluent cell layer and 3D collagen-embedded multicellular spheroid. We use two human carcinoma lines, bladder EJ colorectal HCT116. In 2-D layer, for both HCT migratory speeds effective diffusion coefficients null were significantly smaller than in p53-expressing cells. Compared...
At the edge of a confluent cell layer, cell-free empty space is cue that can drive directed collective cellular migration. Similarly, contact guidance also robust mechanical However, it unclear which two effects stronger, and how each mechanism affects To address this question, here we explore trajectories cells migrating collectively on substrate containing micropatterned grooves (10-20 μm in periodicity, 2 height) compared with unpatterned control substrates. Compared controls, substrates...
Understanding mechanisms underlying various physiological and pathological processes often requires accurate fully automated analysis of dense cell populations that collectively migrate. In such multicellular systems, there is a rising interest in the relations between biophysical cycle progression aspects. A seminal tool led to leap real-time study fluorescent ubiquitination-based indicator (FUCCI). Here, we introduce ConfluentFUCCI, an open-source graphical user interface-based framework...
The formation of an integrated tissue from individual cells depends on the properties as well interaction many acting a collective. Three fundamental physiological processes govern collective scaling cell to working tissue: sorting, assembly, and cellular migration. Mechanistically, sorting is governed by differential adhesion, whereas assembly controlled epithelial-to-mesenchymal transition its inverse, mesenchymal-to-epithelial transition. mechanism driving migration, however, not clear....
Summary Loss of function the tumor suppressor p53 is generally thought to increase cell motility and invasiveness. Using 2-D confluent 3-D spheroidal assays with bladder carcinoma cells colorectal cells, we report, contrary, that loss can decrease invasion. Abstract For migration single studied in isolation, motility. Here by contrast used layer multicellular spheroid investigate how impacts dissemination invasion cellular collectives. We two human lines, EJ HCT116. began replicating...
Abstract Understanding mechanisms underlying various physiological and pathological processes requires accurate fully automated analysis of dense cell populations that collectively migrate, specifically, relations between biophysical features cycle progression aspects. A seminal tool led to a leap in real-time study is the fluorescent ubiquitination-based indicator (FUCCI). Here, we introduce ConfluentFUCCI, an open-source graphical user interface-based framework designed for progression,...