A5086025452- Cellular Mechanics and Interactions
- Neonatal Respiratory Health Research
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Inhalation and Respiratory Drug Delivery
- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Microfluidic and Bio-sensing Technologies
- Axon Guidance and Neuronal Signaling
- Extracellular vesicles in disease
- Mathematical Biology Tumor Growth
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Spaceflight effects on biology
- Biometric Identification and Security
- Cell Adhesion Molecules Research
- Ultrasound and Hyperthermia Applications
- Advanced Biosensing Techniques and Applications
- RNA Research and Splicing
- Medical Imaging and Pathology Studies
- Molecular Biology Techniques and Applications
- Tracheal and airway disorders
- Cell Image Analysis Techniques
- Micro and Nano Robotics
- Forensic Toxicology and Drug Analysis
Harvard University
1997-2024
Wesleyan University
2022-2024
Brigham and Women's Hospital
1997-2023
Boston University
2018
Harvard University Press
2016
Brown University
2010-2013
John Brown University
2010
Maine Medical Center
1999
Maine Medical Center Research Institute
1999
Abstract The epithelial-to-mesenchymal transition (EMT) and the unjamming (UJT) each comprises a gateway to cellular migration, plasticity remodeling, but extent which these core programs are distinct, overlapping, or identical has remained undefined. Here, we triggered partial EMT (pEMT) UJT in differentiated primary human bronchial epithelial cells. After triggering UJT, cell-cell junctions, apico-basal polarity, barrier function remain intact, cells elongate align into cooperative...
BackgroundGenetic variants in the chromosomal region 17q21 are consistently associated with asthma. However, mechanistic studies have not yet linked any of to a function that could influence asthma, and as result, identity asthma gene(s) remains elusive.ObjectivesWe sought identify characterize functional locus.MethodsWe used Exome Aggregation Consortium browser coding (amino acid–changing) locus. We obtained association measures for these both Genetic Epidemiology Research Adult Health...
It is well established that the early malignant tumor invades surrounding extracellular matrix (ECM) in a manner depends upon material properties of constituent cells, ECM, and their interactions. Recent studies have capacity invading spheroids to evolve into coexistent solid-like, fluid-like, gas-like phases. Using breast cancer cell lines engineered here we show spheroid interior develops spatial temporal heterogeneities phase which, depending type density, ultimately result variety...
Mucus overproduction is a major contributor to morbidity and mortality in asthma. induced by orchestrated actions of multiple factors that include inflammatory cytokines γ-aminobutyric acid (GABA). GABA produced only pulmonary neuroendocrine cells (PNECs) the mouse lung. Recent studies neonatal model allergic inflammation have shown PNECs play an essential role mucus hypersecretion. Whether mediate dysregulated signaling for asthma unknown. In this study, we characterized cellular source...
From coffee beans flowing in a chute to cells remodeling living tissue, wide variety of close‐packed collective systems –both inert and living– have the potential jam. The can sometimes flow like fluid or jam rigidify solid. unjammed‐to‐jammed transition remains poorly understood, however, structural properties characterizing these phases remain unknown. Using primary human bronchial epithelial cells, we show that jamming asthma is linked cell shape, thus establishing system criterion for...
Abstract Bronchospasm compresses the bronchial epithelium, and this compressive stress has been implicated in asthma pathogenesis. However, molecular mechanisms by which alters pathways relevant to disease are not well understood. Using air-liquid interface cultures of primary human epithelial cells derived from non-asthmatic donors asthmatic donors, we applied a then used network approach map resulting changes interactome. In compression itself was sufficient induce inflammatory, late...
Abstract In development of an embryo, healing a wound, or progression carcinoma, requisite event is collective epithelial cellular migration. For example, cells at the advancing front wound edge tend to migrate collectively, elongate substantially, and exert tractions more forcefully compared with many ranks behind. With regards energy metabolism, striking spatial gradients have recently been reported in wounded epithelium, as well tumor, but within cell layer little known about link between...
Directed migration of Schwann cells (SC) is critical for development and repair the peripheral nervous system. Understanding aspects motility specific to SC, along with SC response engineered biomaterials, may inform strategies enhance nerve regeneration. Rat were cultured on laminin-coated microgrooved poly(dimethyl siloxane) platforms that flat or presented repeating cellular scale anisotropic topographical cues, 30 60 µm in width, observed timelapse microscopy. motion was directed...
During acute bronchoconstriction, the airway epithelium becomes mechanically compressed, as smooth muscle contracts and narrows. This mechanical compression activates to promote asthmatic remodeling. However, whether compressed can feed back on cause of bronchoconstriction has remained an open question. Here we examine potential for epithelial augment proliferation contraction muscle, thus potentiate further compression. Well-differentiated primary human bronchial (HBE) cells maintained in...
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The healthy and mature epithelial layer is ordinarily quiescent, non-migratory, solid-like, jammed. However, in a variety of circumstances the transitions to phase that dynamic, migratory, fluid-like unjammed. This has been demonstrated developing embryo, avian airway, reconstituted
Temporal RNA-seq data reveal genomic signatures of the unjamming transition in compressed human bronchial epithelial cells.
The COVID-19 pandemic is an ongoing threat to public health. Since the identification of COVID-19, disease caused by SARS-CoV-2, no drugs have been developed specifically target SARS-CoV-2. To develop effective and safe treatment options, a better understanding cellular mechanisms underlying SARS-CoV-2 infection required. fill this knowledge gap, researchers require reliable experimental systems that express host factor proteins necessary for entry These include viral receptor,...
Aberrant remodeling of the asthmatic airway is not well understood but thought to be attributable in part mechanical compression epithelial cells. Here, we examine compression-induced expression and secretion extracellular matrix protein tenascin C (TNC) from well-differentiated primary human bronchial (HBE) cells grown an air-liquid interface culture. We measured TNC mRNA using RT-qPCR secreted Western blotting ELISA. To determine intracellular signaling pathways, used specific inhibitors...
Abstract Every organ surface and body cavity is lined by a confluent collective of epithelial cells. In homeostatic circumstances the remains effectively solid-like sedentary. But during morphogenesis, remodeling or repair, as well malignant invasion metastasis, becomes fluid-like migratory 1–4 . This conversion from sedentary to behavior has traditionally been understood manifestation epithelial-to-mesenchymal transition (EMT) partial EMT (pEMT) 5–8 However, in certain contexts this...
Loss of function the tumor suppressor p53 is known to increase rate migration cells transiting narrow pores traditional Boyden chamber assay. Here by contrast we investigate how impacts cellular within a 2D confluent cell layer and 3D collagen-embedded multicellular spheroid. We use two human carcinoma lines, bladder EJ colorectal HCT116. In 2-D layer, for both HCT migratory speeds effective diffusion coefficients null were significantly smaller than in p53-expressing cells. Compared...