A5026221861- Neonatal Respiratory Health Research
- Asthma and respiratory diseases
- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Inhalation and Respiratory Drug Delivery
- IL-33, ST2, and ILC Pathways
- 3D Printing in Biomedical Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Plant and Fungal Interactions Research
- Diverse Approaches in Healthcare and Education Studies
- Plant Virus Research Studies
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Family and Disability Support Research
- Health and Wellbeing Research
- Mathematical Biology Tumor Growth
- Inflammasome and immune disorders
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Medical Imaging and Pathology Studies
- Piperaceae Chemical and Biological Studies
- Tracheal and airway disorders
- RNA modifications and cancer
- Microbial Natural Products and Biosynthesis
Harvard University
2015-2025
Brigham and Women's Hospital
2023
Kangwon National University
2010-2019
Boston University
2018
International Vaccine Institute
2017
Korea Institute of Radiological and Medical Sciences
2014-2017
Hanil General Hospital
2016
Dongguk University
2015-2016
Jeonbuk National University
2011-2013
Chonbuk National University Hospital
2009-2011
Abstract The epithelial-to-mesenchymal transition (EMT) and the unjamming (UJT) each comprises a gateway to cellular migration, plasticity remodeling, but extent which these core programs are distinct, overlapping, or identical has remained undefined. Here, we triggered partial EMT (pEMT) UJT in differentiated primary human bronchial epithelial cells. After triggering UJT, cell-cell junctions, apico-basal polarity, barrier function remain intact, cells elongate align into cooperative...
BackgroundGenetic variants in the chromosomal region 17q21 are consistently associated with asthma. However, mechanistic studies have not yet linked any of to a function that could influence asthma, and as result, identity asthma gene(s) remains elusive.ObjectivesWe sought identify characterize functional locus.MethodsWe used Exome Aggregation Consortium browser coding (amino acid–changing) locus. We obtained association measures for these both Genetic Epidemiology Research Adult Health...
Lung fibroblast functions such as matrix remodeling and activation of latent transforming growth factor-β1 (TGF-β1) are associated with expression the myofibroblast phenotype directly linked to capacity generate force deform extracellular matrix. However, study force-generating capacities through methods traction microscopy is hindered by low throughput time-consuming procedures. In this study, we improved at detail level for higher-throughput measurements on polyacrylamide hydrogels using...
The widespread use of electronic cigarettes (e-cigarettes or e-cig) is a growing public health concern. Diacetyl and its chemical cousin 2,3-pentanedione are commonly used to add flavors e-cig; however, little known about how the flavoring chemicals may impair lung function. Here we report that induce transcriptomic changes perturb cilia function in airway epithelium. Using RNA-Seq, identified total 163 568 differentially expressed genes primary normal human bronchial epithelial (NHBE) cells...
Abstract The airway epithelium serves as the interface between host and external environment. In many chronic lung diseases, is site of substantial remodeling after injury. While, idiopathic pulmonary fibrosis (IPF) has traditionally been considered a disease alveolus matrix, dominant environmental (cigarette smoking) genetic (gain function MUC5B promoter variant) risk factor primarily affect distal epithelium. Moreover, airway-specific pathogenic features IPF include bronchiolization...
It is well established that the early malignant tumor invades surrounding extracellular matrix (ECM) in a manner depends upon material properties of constituent cells, ECM, and their interactions. Recent studies have capacity invading spheroids to evolve into coexistent solid-like, fluid-like, gas-like phases. Using breast cancer cell lines engineered here we show spheroid interior develops spatial temporal heterogeneities phase which, depending type density, ultimately result variety...
Changes in lung ACE2 expression and apoptotic priming throughout life span may affect COVID severity.
In asthma, tissue factor (TF) levels are elevated in the lung. our previous studies using mechanically compressed human bronchial epithelial (HBE) cells, which a well-defined vitro model of bronchoconstriction during asthma exacerbations, we detected TF within extracellular vesicles (EVs) released from HBE cells. Here, to better characterize potential role this mechanism tested extent transcriptional regulation cell-derived varied between donors with and without asthma. Using RNA situ...
Increased abundance of mucin secretory cells is a characteristic feature the epithelium in asthma and other chronic airway diseases. We showed previously that mechanical stresses constriction, both intact mouse lung cell culture model, activate epidermal growth factor receptor (EGFR), known modulator expression epithelial cells. Here we tested whether chronic, intermittent, short-duration compressive stress (30 cm H(2)O) sufficient to increase MUC5AC-positive intracellular levels human...
Rationale Respiratory virus-induced inflammation is the leading cause of asthma exacerbation, frequently accompanied by induction interferon-stimulated genes ( ISGs ). How asthma-susceptibility modulate cellular response upon viral infection fine-tuning ISG and subsequent airway in genetically susceptible patients remains largely unknown. Objectives To decipher functions gasdermin B (encoded GSDMB ) respiratory lung inflammation. Methods In two independent cohorts, we analysed expression...
The chitinase-like protein YKL-40, encoded by the CHI3L1 gene, is a biomarker and functional effector of chronic inflammatory allergic diseases. In lung it associated with asthma severity reduced function. cellular sources YKL-40 in human airways mechanisms regulating expression are poorly understood. We previously showed that mechanical stress similar to experienced during bronchoconstriction triggers epithelial cell signaling through epidermal growth factor receptor (EGFR), fibrotic...
Mucus overproduction is a major contributor to morbidity and mortality in asthma. induced by orchestrated actions of multiple factors that include inflammatory cytokines γ-aminobutyric acid (GABA). GABA produced only pulmonary neuroendocrine cells (PNECs) the mouse lung. Recent studies neonatal model allergic inflammation have shown PNECs play an essential role mucus hypersecretion. Whether mediate dysregulated signaling for asthma unknown. In this study, we characterized cellular source...
Abstract Bronchospasm compresses the bronchial epithelium, and this compressive stress has been implicated in asthma pathogenesis. However, molecular mechanisms by which alters pathways relevant to disease are not well understood. Using air-liquid interface cultures of primary human epithelial cells derived from non-asthmatic donors asthmatic donors, we applied a then used network approach map resulting changes interactome. In compression itself was sufficient induce inflammatory, late...
Histological and lineage immunofluorescence examination revealed that healthy conducting airways of humans animals harbor sporadic poorly differentiated epithelial patches mostly in the dorsal noncartilage regions remarkably manifest squamous differentiation.