A5037838174- Air Quality and Health Impacts
- Preterm Birth and Chorioamnionitis
- Pregnancy and preeclampsia studies
- Per- and polyfluoroalkyl substances research
- MicroRNA in disease regulation
- Reproductive System and Pregnancy
- Single-cell and spatial transcriptomics
- Neonatal and Maternal Infections
- Pregnancy-related medical research
- Endoplasmic Reticulum Stress and Disease
- Sulfur Compounds in Biology
- Toxic Organic Pollutants Impact
- Trace Elements in Health
- Smoking Behavior and Cessation
- Pancreatic and Hepatic Oncology Research
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Genomics, phytochemicals, and oxidative stress
- Phytoestrogen effects and research
- Computational Drug Discovery Methods
- Circular RNAs in diseases
- Genetics and Neurodevelopmental Disorders
- Aluminum toxicity and tolerance in plants and animals
- Environmental Justice and Health Disparities
- Sirtuins and Resveratrol in Medicine
University of Rochester
2021-2024
University of Michigan
2011-2020
Harvard University
2016-2019
Environmental Health
2016
Yonsei University
2010-2013
The University of Tokyo
2004
Background: Glucose deprivation, a feature of poorly vascularized solid tumors, activates the unfolded protein response (UPR), stress-signaling pathway, in tumor cells. We recently isolated novel macrocyclic compound, versipelostatin (VST), that inhibits transcription from promoter GRP78, gene is activated as part UPR. examined effect VST on UPR induced by glucose deprivation or other stressors and growth vivo. Methods: Human colon cancer HT-29, fibrosarcoma HT1080, stomach MKN74 cells were...
BackgroundGenetic variants in the chromosomal region 17q21 are consistently associated with asthma. However, mechanistic studies have not yet linked any of to a function that could influence asthma, and as result, identity asthma gene(s) remains elusive.ObjectivesWe sought identify characterize functional locus.MethodsWe used Exome Aggregation Consortium browser coding (amino acid–changing) locus. We obtained association measures for these both Genetic Epidemiology Research Adult Health...
The widespread use of electronic cigarettes (e-cigarettes or e-cig) is a growing public health concern. Diacetyl and its chemical cousin 2,3-pentanedione are commonly used to add flavors e-cig; however, little known about how the flavoring chemicals may impair lung function. Here we report that induce transcriptomic changes perturb cilia function in airway epithelium. Using RNA-Seq, identified total 163 568 differentially expressed genes primary normal human bronchial epithelial (NHBE) cells...
Significance Dysregulated endoplasmic reticulum (ER) stress response contributes to the pathogenesis of myriad diseases. The molecular pathways leading ER stress-induced cell death are well characterized; however, much less is known about how cells suppress excessive avoid apoptosis and restore homeostasis. Using a CRISPR-based loss-of-function genetic screen, our study uncovered multiple suppressors response. These include polycomb protein complex that directly inhibits expression...
Background:Lead (Pb) adversely affects neurodevelopment in children. Neural stem cells (NSCs) play an essential role shaping the developing brain, yet little is known about how Pb perturbs NSC functions and whether such perturbation contributes to impaired neurodevelopment.Objectives:We aimed identify Pb-induced transcriptomic changes NSCs link these neurodevelopmental outcomes children who were exposed Pb.Methods:We performed RNA-seq-based profiling human treated with 1 μM Pb. We used...
Trichloroethylene (TCE) is a common environmental pollutant associated with adverse reproductive outcomes in humans. TCE intoxication occurs primarily through its biotransformation to bioactive metabolites, including S-(1,2-dichlorovinyl)-l-cysteine (DCVC). induces oxidative stress and inflammation the liver kidney. Although placenta capable of xenobiotic metabolism have been pregnancy outcomes, toxicity remains poorly understood. We determined effects DCVC by using human extravillous...
Exposure to environmental heavy metals such as cadmium (Cd) is often linked neurotoxicity but the underlying mechanisms remain poorly understood. Here we show that Arrestin domain-containing protein 1 (ARRDC1)-mediated microvesicles (ARMMs)–an important class of extracellular vesicles (EVs) whose biogenesis occurs at plasma membrane–protect against Cd-induced neurotoxicity. Cd increased production EVs, including ARMMs, in a human neural progenitor cell line, ReNcell CX (ReN) cells. ReN cells...
The placenta is crucial for fetal development, yet the impact of environmental stressors such as arsenic exposure remains poorly understood. We apply single-cell RNA sequencing to analyze response mouse arsenic, revealing cell-type-specific gene expression, function, and pathological changes. Notably, Prap1 gene, which encodes proline-rich acidic protein 1 (PRAP1), significantly upregulated in 26 placental cell types including various trophoblast cells. Our study shows a female-biased...
Abstract Arsenic (As) exposure adversely affects neurodevelopment in children. Accumulation of misfolded proteins cells exposed to As leads endoplasmic reticulum (ER) stress response, which, if not relieved, results cell death. Despite the potential role ER for As-induced neurotoxicity, underlying mechanisms remain poorly understood. Here we aimed investigate roles microRNA(miR)-124, a novel suppressor, response and cytotoxicity neural cells. We further link these vitro findings...
Streptococcus agalactiae (group B streptococcus [GBS]) infection in pregnant women is the leading cause of infectious neonatal morbidity and mortality United States. Although inflammation during has been associated with preterm birth, contribution GBS to birth less certain. Moreover, early mechanisms by which interacts gestational tissue affect adverse pregnancy outcomes are poorly understood. We hypothesized that short-term inoculation activates pathways related premature human...
Abstract BACKGROUND: Isoflavones are the most common group of phytoestrogens which present in significantly large amounts soybean and soy products such as tofu. occur naturally glycoside forms having lower bioavailability than their aglycone forms. β‐Glycosidase acts a bio‐catalyst for conversion isoflavone glycosides to aglycones, raising isoflavones; therefore, it can be used improve quality We need establish process conditions optimal outcome enzyme reaction RESULTS: By using...
Abstract The widespread use of electronic cigarettes (e-cig) is a serious public health concern; however, mechanisms by which e-cig impair the function airway epithelial cells—the direct target smoke—are not fully understood. Here we report transcriptomic changes, including decreased expression many ribosomal genes, in cells response to exposure. Using RNA-seq identify over 200 differentially expressed genes air–liquid interface cultured primary normal human bronchial (NHBE) exposed smoke...
Infection during pregnancy is a significant public health concern due to the increased risk of adverse birth outcomes. Group B Streptococcus or agalactiae (GBS) stands out as major bacterial cause neonatal morbidity and mortality. We aimed explore involvement reactive oxygen species (ROS) oxidative stress pathways in pro-inflammatory responses within human fetal membrane tissue, target tissue acute chorioamnionitis. reanalyzed transcriptomic data from explants inoculated with GBS assess...
Abstract Group B streptococcus (GBS) infection is a significant public health concern associated with adverse pregnancy complications and increased neonatal mortality morbidity. However, the mechanisms underlying impact of GBS on fetal membrane, first line defense against pathogens, are not fully understood. Here, we propose that induces senescence inflammatory factors (IL-6 IL-8) in membrane through interleukin-1 (IL-1). Utilizing existing transcriptomic data GBS-exposed human showed...
Background: Carefully regulated cytokine production during pregnancy by trophoblasts and neighboring cells in the placenta extra-placental membranes is integral for successful gestation. Polybrominated diphenyl ethers (PBDEs) are commonly found as flame retardants electronic devices, textiles, other products. Brominated ether-47 (BDE-47) a PBDE congener that bioaccumulates human breast milk, fetal serum, maternal serum placenta. Despite presence of PBDEs importance regulation parturition,...