A5041685713- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Atherosclerosis and Cardiovascular Diseases
- Mathematical Biology Tumor Growth
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Thermography and Photoacoustic Techniques
- Advanced Biosensing Techniques and Applications
- Advanced Sensor and Energy Harvesting Materials
- Biocrusts and Microbial Ecology
- Biometric Identification and Security
- Laser-Matter Interactions and Applications
- Near-Field Optical Microscopy
- Hepatitis C virus research
- Bee Products Chemical Analysis
- Coronary Interventions and Diagnostics
- Spaceflight effects on biology
- Additive Manufacturing and 3D Printing Technologies
- Advanced Fiber Laser Technologies
- Photonic and Optical Devices
National Research Council Canada
2004-2025
University of Ottawa
2018-2024
Ottawa University
2020
Harvard University
2015-2019
Harvard College Observatory
2019
Queen's University
2009-2012
Steacie Institute for Molecular Sciences
2008-2012
National Academies of Sciences, Engineering, and Medicine
2009-2011
Joint Research Centre
1972-2002
Istituto Superiore per la Protezione e la Ricerca Ambientale
1972
Significance Cell volume is thought to be a well-controlled cellular characteristic, increasing as cell grows, while macromolecular density maintained. We report that can also change in response external physical cues, leading water influx/efflux, which causes significant changes subcellular density. This observed when cells spread out on substrate: Cells reduce their and increase molecular crowding due an accompanying efflux. Exploring this phenomenon further, we removed from mesenchymal...
Copper toxicity is a critical issue in the development of copper-based catalysts for copper(I)-catalyzed azide–alkyne cycloaddition (CuAAC) reactions applications living systems. The effects and related copper on mammalian cells are dependent ligand environment. complexes can be highly toxic, induce changes cellular metabolism, rapidly taken up by cells, all which affect their ability to function as CuAAC Herein, we have evaluated number that typically used catalyze four human cell lines...
Soft, solvent-free poly(dimethylsiloxane) elastomers are fabricated by a one-step process via crosslinking bottlebrush polymers. The architecture prevents the formation of entanglements, resulting in with precisely controllable low moduli from 1 to 100 kPa, below lower limit traditional elastomers; moreover, nature enables their negligible adhesiveness compared commercially available silicone products similar stiffness. As service our authors and readers, this journal provides supporting...
We demonstrate high performance coherent anti-Stokes Raman scattering (CARS) microscopy of live cells and tissues with user-variable spectral resolution broad tunability (2500 - 4100 cm(-1)), using a femtosecond Ti:Sapphire pump photonic crystal fiber output for the broadband synchronized Stokes pulse. Spectral chirp fs laser pulses was parameter optimization in focusing implementation multimodal CARS microscopy. High signal-to-noise, contrast imaging achieved pixel dwell times 2-8 micros...
Recent development of liquid phase transmission electron microscopy (TEM) enables the study specimens in wet ambient conditions within a cell; however, direct structural observation biological samples their native solution using TEM is challenging since low-mass biomaterials embedded thick layer host cell demonstrate low contrast. Furthermore, integrity delicate easily compromised during typical sample preparation and imaging. To overcome these limitations, we introduce graphene (GLC)...
It is well established that the early malignant tumor invades surrounding extracellular matrix (ECM) in a manner depends upon material properties of constituent cells, ECM, and their interactions. Recent studies have capacity invading spheroids to evolve into coexistent solid-like, fluid-like, gas-like phases. Using breast cancer cell lines engineered here we show spheroid interior develops spatial temporal heterogeneities phase which, depending type density, ultimately result variety...
In eukaryotes, the cell volume is observed to be strongly correlated with nuclear volume. The slope of this correlation depends on type, growth condition, and physical environment cell. We develop a computational model proteome increase, incorporating kinetics amino acid import, protein/ribosome synthesis degradation, active transport proteins between cytoplasm nucleoplasm. also include simple ribosome biogenesis assembly. Results show that tightly volume, cytoplasm-nucleoplasm rates...
Using an all-fiber laser system consisting of a femtosecond Er/Yb fiber oscillator as the pump and ultra-highly nonlinear for Stokes generation, we demonstrate multimodal (TPF+SHG+CARS) optical microscopy both tissue samples live cells.Multimodal imaging was successfully performed with pixel dwell times short 4 μs at low powers (< 40 mW total).
Within multicellular living systems, cells coordinate their positions with spatiotemporal accuracy to form various tissue structures and control development. These arrangements can be regulated by geometry, biochemical cues, as well mechanical perturbations. However, how pack during dynamic three-dimensional architectures formation remains unclear. Here, examining a growing spherical system, human lung alveolospheres, we observe an emergence of hexagonal packing order structural transition...
Abstract A simple scheme for multimodal coherent anti‐Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast‐oscillator‐derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber‐generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throughout CH/OH high frequency region (2700–4000 cm ‐1 ). Here we extend this approach to middle (1640–3300 ) fingerprint regions (850–1800 spectrum. Our integrated CARS in...
We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for study structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS was used to elucidate structure, alignment, density in cellulose cotton fibers starch grains undergoing rapid heat-moisture swelling. Our results suggest that response O-H stretch region (3000 cm(-1)-3400 cm(-1)), together with commonly-measured C-H (2750 cm(-1)-2970...
We investigate the role of a spatially inhomogenous nonresonant background medium on several Raman-based imaging modalities. In particular, we consider small resonant bead submerged in heterogeneous χ(3) background. Using detailed 3D electrodynamic simulations, compare coherent anti-Stokes Raman scattering (CARS), frequency-modulated CARS, amplitude-modulated stimulated (SRS), and SRS. find that only FM-SRS is background-free.