A5016846535- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Mast cells and histamine
- Urticaria and Related Conditions
- Asthma and respiratory diseases
- Neuropeptides and Animal Physiology
- Dermatology and Skin Diseases
- Receptor Mechanisms and Signaling
- Allergic Rhinitis and Sensitization
- Immune Response and Inflammation
- Advancements in Transdermal Drug Delivery
- IL-33, ST2, and ILC Pathways
- Cell Adhesion Molecules Research
- Olfactory and Sensory Function Studies
- Liver physiology and pathology
- Chemokine receptors and signaling
- Pain Mechanisms and Treatments
- Contact Dermatitis and Allergies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Antimicrobial Peptides and Activities
- Pharmacological Receptor Mechanisms and Effects
- Reproductive System and Pregnancy
- Adenosine and Purinergic Signaling
- Cancer, Stress, Anesthesia, and Immune Response
University of Pittsburgh
2016-2025
Riverside Transplantation Institute
2009
Indiana University School of Medicine
2004-2007
Indiana University – Purdue University Indianapolis
2004-2007
Bipar
2004
University of Michigan
2000-2002
Regional West Medical Center
2002
Itch is a dominant symptom in dermatitis, and scratching promotes cutaneous inflammation, thereby worsening disease. However, the mechanisms through which exacerbates inflammation whether provides benefit to host are largely unknown. We found that was required for skin mouse models dependent on FcεRI-mediated mast cell activation. Scratching-induced pain-sensing nociceptors, neuropeptide substance P, receptor MrgprB2. Scratching also increased augmented defense superficial Staphylococcus...
Obesity-associated increases in adipose tissue (AT) CD11c(+) cells suggest that dendritic (DC), which are involved the recruitment and activation of macrophages, may play a role determining AT liver immunophenotype obesity. This study addressed this hypothesis. With use flow cytometry, electron microscopy, loss-and-gain function approaches, contribution DC to pattern immune cell alterations obesity was assessed. In there substantial, high-fat diet (HFD)-induced increase DC. AT, these were...
T cell (or transmembrane) immunoglobulin and mucin domain protein 3 (Tim-3) has attracted significant attention as a novel immune checkpoint receptor (ICR) on chronically stimulated, often dysfunctional, cells. Antibodies to Tim-3 can enhance antiviral antitumor responses. is also constitutively expressed by mast cells, NK cells specific subsets of macrophages dendritic There ample evidence for positive role in these latter types, which at odds with the model an inhibitory molecule At this...
Abstract Minimization of immunosuppression and donor-specific tolerance to MHC-mismatched organ grafts are important clinical goals. The therapeutic potential regulatory T cells (Tregs) has been demonstrated, but conditions for optimizing their in vivo function posttransplant nonlymphocyte-depleted hosts remain undefined. In this study, we address mechanisms through which inhibition the mammalian target rapamycin (Rapa) synergizes with alloantigen-specific Treg (AAsTreg) permit long-term,...
Plasmacytoid dendritic cells (pDCs) are highly specialized APCs that, in addition to their well-recognized role anti-viral immunity, also regulate immune responses. Liver-resident pDCs considerably less immunostimulatory than those from secondary lymphoid tissues and equipped promote tolerance/regulation through various mechanisms. IL-27 is an IL-12 family cytokine that regulates the function of both T cells, although little known about its pDC immunobiology. In this study, we show mouse...
Abstract Hepatic stellate cells (HSCs) may play an important role in hepatic immune regulation by producing numerous cytokines/chemokines and expressing Ag-presenting T cell coregulatory molecules. Due to disruption of the endothelial barrier during cold-ischemic storage reperfusion liver grafts, HSCs can interact directly with system. Endotoxin (LPS), levels which increase diseases transplantation, stimulates synthesis many mediators HSCs. We hypothesized that LPS-stimulated might promote...
The nucleotide-binding oligomerization domain (NOD)2/CARD15 protein, which senses muramyl dipeptide (MDP), a product of bacterial peptidoglycan, appears to play an important role in regulating intestinal immunity. Although the liver is exposed gut-derived MDP, influence NOD2 ligation on hepatic APC, particular dendritic cells (DC), unknown. Freshly isolated mouse and spleen plasmacytoid (p)DC expressed higher levels message than conventional myeloid (m)DC. Following MDP stimulation vivo,...
Abstract Maturation resistance and tolerogenic properties can be conferred on human murine dendritic cells (DC), crucial regulators of T cell responses, by exposure to rapamycin (RAPA), a “tolerance-sparing” immunosuppressive agent. Mechanisms underlying this acquired unresponsiveness, typified diminished functional responses TLR or CD40 ligation, have not been identified. We report that in vitro vivo conditioning myeloid DC with RAPA elicits the de novo production IL-1β otherwise...
Hepatic stellate cells (HSCs) are critical for hepatic wound repair and tissue remodeling. They also produce cytokines chemokines that may contribute to the maintenance of immune homeostasis inherent tolerogenicity liver. The functional relationship between HSCs professional migratory APCs in liver, is, dendritic (DCs), has not been evaluated. In this article, we report murine liver DCs colocalize with vivo under normal, steady-state conditions, cluster vitro. vitro, secrete high levels DC...
A critical goal in transplantation is the achievement of donor-specific tolerance, minimizing use immunosuppressants. Dendritic cells (DCs) are antigen (Ag) presenting (APCs) with capability to promote immunity or tolerance. The immune-regulatory properties DCs have been exploited for generation tolerogenic/immunosuppressive (IS) that, when transfer systemically, prolong allograft survival murine models. Surprisingly, vivo mechanisms therapies based on (donor- recipient-derived) ISDCs remain...
Efficient Ca2+ flux induced during cognate T cell activation requires signaling the receptor (TCR) and unidentified G-protein-coupled receptors (GPCRs). cells express neurokinin-1 (NK1R), a GPCR that mediates in excitable non-excitable cells. However, role of NK1R TCR remains unknown. We show its agonists, neuropeptides substance P hemokinin-1, co-localize within immune synapse Simultaneous stimulation is necessary for efficient Ca2+-dependent sustains survival activated helper 1 (Th1) Th17...
Psoriasis is a chronic inflammatory skin disease with no cure. Although the origin of psoriasis and its underlying pathophysiology remain incompletely understood, inflammation central mediator progression. In this regard, electrophilic nitro-fatty acids (NO2–FAs) exert potent anti-inflammatory effects in several vivo murine models diseases, such as kidney cardiovascular disease. To examine therapeutic potential NO2–FAs on psoriasiform dermatitis, we employed multiple psoriasis. Our studies...