A5030396783- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Liver physiology and pathology
- Liver Disease and Transplantation
- Immune Response and Inflammation
- Transplantation: Methods and Outcomes
- Xenotransplantation and immune response
- Conducting polymers and applications
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- Synthesis and properties of polymers
- Cancer Immunotherapy and Biomarkers
- Poxvirus research and outbreaks
- Organic Electronics and Photovoltaics
- Hepatitis B Virus Studies
- Bacillus and Francisella bacterial research
- Diabetes and associated disorders
- HIV, Drug Use, Sexual Risk
- Bladder and Urothelial Cancer Treatments
- RNA Interference and Gene Delivery
- Renal cell carcinoma treatment
- Liver Diseases and Immunity
National Center for Global Health and Medicine
2023-2025
Tokyo Women's Medical University
2010-2025
Center for Global Health
2024
University of Pittsburgh
2006-2012
Hiroshima University
1997-2012
University of Pittsburgh Medical Center
2006-2008
Nagoya University
2004
Hiroshima Prefectural Hospital
1999
Abstract More effective discrimination between CD4+CD25+ regulatory T cells (Treg) and activated would significantly improve the current level of purification Treg their therapeutic application. We observed that ∼90% (positive for nuclear transcription factor Forkhead winged helix protein-3 able to inhibit naive cell proliferation) isolated from spleens or lymph nodes normal mice did not express significant levels inhibitory receptor programmed death-1 (PD-1) on surface, but retained PD-1...
Background. Both dendritic cells (DC) and T-regulatory (Treg) have been implicated in regulation of alloimmune responses transplant tolerance. Methods. We analyzed B7 coregulatory molecule expression on circulating DC subset precursors, together with CD4+CD25hi Foxp3+ Treg by rare event, flow cytometric analysis operationally tolerant pediatric liver recipients (TOL), those undergoing prospective immunosuppressive drug weaning (PW) or maintenance immunosuppression (MI), normal healthy...
Although it is known that activation of natural killer (NK) cells causes liver injury, the mechanisms underlying NK cell-induced killing self-hepatocytes are not clear. We demonstrated have cytotoxicity against normal syngeneic hepatocytes in mice. Polyinosinic-polycytidylic acid (poly I:C) treatment enhanced hepatocyte toxicity but spleen cells. Unlike other tissues, approximately 30%-40% constitutively express tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). An vitro cell...
The nucleotide-binding oligomerization domain (NOD)2/CARD15 protein, which senses muramyl dipeptide (MDP), a product of bacterial peptidoglycan, appears to play an important role in regulating intestinal immunity. Although the liver is exposed gut-derived MDP, influence NOD2 ligation on hepatic APC, particular dendritic cells (DC), unknown. Freshly isolated mouse and spleen plasmacytoid (p)DC expressed higher levels message than conventional myeloid (m)DC. Following MDP stimulation vivo,...
Abstract Proinflammatory responses play critical roles in hepatic ischemia/reperfusion (I/R) injury associating with liver transplantation (LTx), and carbon monoxide (CO) can effectively down-regulate them. Using wild-type (WT) to enhanced green fluorescent protein (EGFP)-transgenic rat LTx 18-hour cold preservation University of Wisconsin solution, this study analyzed the relative contribution donor host cells during early posttransplantation period elucidated mechanism protection by CO. CO...
Abstract Background Since May 2022, the incidence of mpox cases has surged outside endemic regions. Although vaccination remains pivotal in prevention, data on LC16m8, a third-generation smallpox vaccine, are scant. We provided LC16m8 pre-exposure prophylaxis opportunities to high-risk individuals, including those with HIV, and conducted randomized controlled trial assess LC16m8’s effectiveness prevention safety. Methods Our multicenter open-label enrolled men women aged ≥18 years at high...
Abstract Background This randomized controlled trial provided LC16m8 pre-exposure prophylaxis to high-risk individuals assess its efficacy for mpox prevention, safety, and immunogenicity. Methods multicenter, randomized, open-label enrolled men women aged ≥18 years at high risk of mpox. Participants were randomly assigned 1:1 early- or late-vaccination groups. The primary endpoint was vaccine (VE) against Secondary endpoints included VE severe mpox, symptoms, “take” incidence, adverse events...
Abstract Although livers transplanted across MHC barriers in mice are normally accepted without recipient immune suppression, the underlying mechanisms remain to be clarified. To identify cell type that contributes induction of such a tolerance state, we established mixed hepatic constituent cell-lymphocyte reaction (MHLR) assay. Irradiated C57BL/6 (B6) or BALB/c mouse cells (HCs) and CFSE-labeled B6 splenocytes were cocultured. In allogeneic MHLR, whole HCs did not promote T proliferation....
DC are believed to play important roles in the induction and regulation of immune responses liver, an organ implicated peripheral tolerance. Since liver is located downstream gut, it constantly exposed bacterial LPS. Our recent observations indicate that prior exposure endotoxin modulates subsequent this TLR4 ligand. In study, we demonstrate modifies capacity mouse myeloid (MDC) activated by CpG (TLR9 ligand) direct Th1-type responses. IL-12 production MDC was significantly lower than spleen...
An understanding of the mechanisms that suppress human anti-pig cellular response is key for xenotransplantation. We have compared ability regulatory T cells (Tregs) to xenogeneic and allogeneic responses in vitro.Human peripheral blood mononuclear (PBMC), CD4+ cells, or CD25- were stimulated with irradiated wild type (WT) alpha1,3-galactosyltransferase gene-knockout (GT-KO) pig PBMC presence absence CD25 high Tregs. In separate experiments, 5- (and 6)-carboxyfluorescein diacetate...
Background. Immature dendritic cells (DC) can promote long-term transplant survival in rodents. We assessed the impact of stably immature, donor-derived DC on alloimmune reactivity rhesus macaques. Methods. CD14+ monocytes isolated from leukapheresis products Macacca mulatta were cultured granulocyte-macrophage colony stimulating factor plus interleukin (IL)-4±vitamin (vit) D3, and IL-10. Major histocompatibility complex class II cosignaling molecule expression was determined CD11c+ by flow...
A portal venous injection of allogeneic donor cells is known to prolong the survival subsequently transplanted allografts. In this study, we investigated role liver sinusoidal endothelial (LSECs) in immunosuppressive effects induced by a on T with indirect allospecificity. To eliminate direct CD4+ cell response, C57BL/6 (B6) MHC class II-deficient C2tatm1Ccum (C2D) mice were used as donors. After irradiated B6 C2D splenocytes into BALB/c mice, host LSECs that endocytosed showed enhanced...
Prophylaxis of antibody-mediated rejection (AMR) caused by donor-specific antibodies remains challenging. Given the critical roles complement activity in graft injury, we developed a lipid nanoparticle (LNP) formulation small-interfering RNA against C5 (C5 siRNA-LNP) and investigated whether siRNA-LNP could downregulate act as an effective treatment for AMR.Lewis recipient rats were sensitized skin grafting from Brown Norway donor rats. Kidney transplantation was performed at 4 wk post-skin...
Abstract Background The incidence of mpox cases has surged outside endemic regions since May 2022. However, data regarding the safety and efficacy LC16m8 vaccine are limited. This study provided opportunities for pre-exposure prophylaxis to high-risk individuals conducted a randomized controlled trial assess effectiveness in prevention. Methods multicenter, randomized, open-label enrolled men women aged ≥18 with high risk. Participants were randomly assigned 1:1 early or late vaccination...
Kidney allograft rejections are orchestrated by a variety of immune cells. Because the complex histopathologic features, accurate pathological diagnosis poses challenges even for expert pathologists. The objective this study was to unveil novel spatial indices associated with transplant rejection using bioinformatic approach 36-plex immunofluorescence image data.
To evaluate the role of oral glucose tolerance test (OGTT) before transplantation and to examine risk factors for new-onset diabetes after (NODAT) during long-term follow-up renal transplant recipients receiving FK-based therapy.The study evaluated 378 patients pre-transplantation using OGTT assigned them one three groups: Group 1, normal pattern; 2, impaired fasting (IFG)/impaired (IGT) pattern (IFG/IGT); 3, DM pattern.Although incidence NODAT was higher in 3 than groups 1 no significant...
With the coronavirus disease 2019 (COVID-19) pandemic, there is growing interest in utilizing adaptive platform clinical trials (APTs), which multiple drugs are compared with a single common control group, such as placebo or standard-of-care group. APTs evaluate several for one and accept additions exclusions of progress; however, little known about efficiency over stand-alone trials. In this study, we simulated total development period, sample size, statistical operating characteristics...