A5008287349- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Colorectal Cancer Surgical Treatments
- Gastric Cancer Management and Outcomes
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Xenotransplantation and immune response
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Gallbladder and Bile Duct Disorders
- Immunotherapy and Immune Responses
- Liver physiology and pathology
- Colorectal Cancer Treatments and Studies
- Cancer Immunotherapy and Biomarkers
- Cytomegalovirus and herpesvirus research
- Pancreatic function and diabetes
- Surgical Simulation and Training
- Transplantation: Methods and Outcomes
Hiroshima University Hospital
2016-2025
Hiroshima University
2016-2025
Higashihiroshima Medical Center
2010-2025
The Japanese Society of Gastroenterological Surgery
2016-2025
Kyushu Okinawa Agricultural Research Center
2011-2024
Nagoya University
2004-2023
University of Copenhagen
2023
Memorial Sloan Kettering Cancer Center
2023
National Institutes of Natural Sciences
2023
Osaka University
2011-2023
Chronic hepatitis C virus (HCV) infection is an important risk factor for hepatocellular carcinoma (HCC). Despite effective antiviral therapies, the HCC decreased but not eliminated after a sustained virologic response (SVR) to direct-acting (DAA) agents, and higher in patients with advanced fibrosis. We investigated HCV-induced epigenetic alterations that might affect DAA treatment mice humanized livers.We performed genome-wide ChIPmentation-based ChIP-Seq RNA-seq analyses of liver tissues...
Intrahepatic cholangiocarcinoma and combined hepatocellular show varying degrees of biliary epithelial differentiation, which can be defined as liver cancer displaying phenotype (LCB). LCB is second in the incidence for cancers with without chronic hepatitis background more aggressive than carcinoma (HCC). To gain insight into its molecular alterations, we performed whole-genome sequencing analysis on 30 LCBs. Here show, genome-wide substitution patterns LCBs developed livers overlapped...
As of 31 December 2017, a total 9242 liver transplants have been carried out in 67 institutions Japan. There were 447 deceased donor (444 from heart‐beating donors and 3 non‐heart‐beating donors) 8795 living‐donor transplants. The annual 2017 was 416 (69 347 transplants). most frequent indication cholestatic disease, followed by neoplastic disease hepatocellular disease. In terms cirrhosis due to hepatitis C B decreased (13 8, respectively), whereas alcoholic markedly increased (32). Patient...
Mutations in TGFBR2, a component of the transforming growth factor (TGF)-β signaling pathway, occur high-frequency microsatellite instability (MSI-H) colorectal cancer (CRC). In mouse models, Tgfbr2 inactivation intestinal epithelium accelerates development malignant tumors combination with disruption Wnt-β-catenin pathway. However, no studies have further identified genes influenced by TGFBR2 following We previously described CDX2P-G19Cre;Apcflox/flox mice, which is stochastically null for...
The aim of this study was to use small unmanned aerial vehicles (UAVs) for determining high-resolution normalized difference vegetation index (NDVI) values. Subsequently, these results were used assess their correlations with fertilizer application levels and the yields rice wheat crops. For multispectral sensing, we flew two types UAVs (DJI Phantom 4 DJI Pro)—each equipped a compact sensor (Parrot Sequoia). information collected composed numerous RGB orthomosaic images as well reflectance...
Abstract This cross-sectional study aimed to investigate the post-acute consequences of COVID-19. We conducted a self-administered questionnaire survey on sequelae, psychological distress (K6), impairments in work performance (WFun), and COVID-19–related experiences stigma discrimination two designated COVID-19 hospitals Hiroshima Prefecture, Japan, between August 2020 March 2021. The prevalence sequelae was calculated by age severity. Factors independently associated with or were identified...
We have previously proven that human macrophages can phagocytose porcine cells even in the absence of Ab or complement opsonization, indicating present a pivotal immunological obstacle to xenotransplantation. A recent report indicates signal regulatory protein (SIRP)α is critical immune inhibitory receptor on macrophages, and its interaction with CD47, ligand for SIRPα, prevents autologous phagocytosis. Considering limited compatibility (73%) amino acid sequences between pig we hypothesized...
Transcriptional initiation is a key step in the control of mRNA synthesis and intimately related to chromatin structure histone modification. Here, we show that ubiquitylation H2A (ubH2A) correlates with silent regulates transcriptional initiation. The levels ubH2A vary during hepatocyte regeneration, based on microarray expression data from regenerating liver, identified USP21, ubiquitin-specific protease catalyzes hydrolysis ubH2A. When assembled vitro, ubH2A, but not H2A, specifically...
Xenotransplantation could overcome the severe shortage of allogeneic organs, a major factor limiting organ transplantation. Unfortunately, transplantation organs from pigs, most suitable potential donor species, results in hyperacute rejection primate recipients, due to presence anti–Galα1-3Gal (Gal) natural antibodies (NAbs) their sera. We evaluated ability tolerize anti-Gal NAb–producing B cells α1,3-galactosyltransferase knockout (GalT KO) mice using bone marrow (BMT) GalT+/+ wild-type...
In rodents, liver natural killer (NK) cells have been shown to mediate higher cytotoxic activity against tumor than do peripheral blood (PB) NK cells. However, such differences between and PB not extensively investigated in humans. The phenotypical functional properties of extracted from perfusates at the time living donor transplantation were investigated. necrosis factor–related apoptosis-inducing ligand (TRAIL), a critical molecule for cell–mediated anti-tumor cell killing, was expressed...
Significance The high-mobility group box 1 (HMGB1) protein is abundantly expressed in the nucleus where it regulates chromatin function. More recently, was found to also function cytoplasm and extracellular milieu for regulation of immunity inflammation. However, vivo study HMGB1 has been hampered by fact that HMGB1-deficient mice die soon after birth. In this study, we successfully generated Hmgb1 -floxed achieve conditional inactivation gene a cell- tissue-specific manner. We demonstrate...
We evaluated the effects of rituximab prophylaxis on outcomes ABO-blood-type-incompatible living donor liver transplantation (ABO-I LDLT) in 381 adult patients Japanese registry ABO-I LDLT. Patients underwent dual or triple immunosuppression with without B cell desensitization therapies such as plasmapheresis, splenectomy, local infusion, intravenous immunoglobulin and rituximab. Era before 2005, intensive care unit-bound status, high Model for End-Stage Liver Disease score absence were...
A retrospective study was performed to clarify the role of conversion therapy (surgery with a prospect R0 resection in initially unresectable metastatic cancer that responded chemotherapy) stage IV gastric (GC). We treated 259 GC patients systemic chemotherapy at Gifu and Hiroshima University Hospitals between 2001–2013. Of these, 84 who were subsequently by surgery classified into four categories according our previously published classification GC, short- long-term outcomes analyzed....
Abstract Background The number of older patients with cancer has increased, and colorectal is expected to be affected by this trend. This study aimed compare prognostic factors, including nutritional inflammation-based indices, between aged ≥ 70 < years following curative resection stage I–III cancer. Patients Methods included 560 who underwent May 2010 June 2018. A retrospective analysis was performed identify prognosis-associated variables in years. Results Preoperative low body mass...
Abstract Peritoneal cavity B-1 cells are believed to produce IgM natural Abs. We have used α1,3-galactosyltransferase-deficient (GalT−/−) mice, which, like humans, Abs against the carbohydrate epitope Galα1,3Gal (Gal), demonstrate that peritoneal B-1b with anti-Gal receptors only after LPS stimulation. Likewise, of GalT−/− and wild-type mice do not other specificities without Development Ab-secreting capacity is associated loss CD11b/CD18 (Mac-1) expression. In contrast, there large numbers...
Although it is known that activation of natural killer (NK) cells causes liver injury, the mechanisms underlying NK cell-induced killing self-hepatocytes are not clear. We demonstrated have cytotoxicity against normal syngeneic hepatocytes in mice. Polyinosinic-polycytidylic acid (poly I:C) treatment enhanced hepatocyte toxicity but spleen cells. Unlike other tissues, approximately 30%-40% constitutively express tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). An vitro cell...
Colon cancer (CC) is a leading cause of mortality. Novel biomarkers are needed to identify CC patients at high risk recurrence and those who may benefit from therapeutic intervention. The aim this study investigate if miR-21 expression RNA isolated formalin-fixed paraffin-embedded (FFPE) tissue sections associated with prognosis outcome for CC. was measured by quantitative reverse transcriptase-polymerase chain reaction in Japanese cohort (stage I-IV, n = 156) German II, 145). High tumors...
Circulating tumor DNA (ctDNA) carrying tumor-specific sequence alterations has been found in the cell-free fraction of blood. Liver cancer specimens are difficult to obtain, and noninvasive methods required assess progression characterize underlying genomic features.We analyzed 46 patients with hepatocellular carcinoma who underwent hepatectomy or liver transplantation for whom whole-genome sequencing data was available. We designed personalized assays targeting somatic rearrangements each...