Significance Hypoxia predisposes otherwise healthy individuals to venous thrombosis, but the underlying mechanism has been unclear. Our study revealed a causal role for nucleotide binding domain, leucine-rich-containing family, pyrin domain containing 3 (NLRP3) inflammasome and IL-1β during hypoxia-induced thrombosis. We further show direct association between NLRP3 hypoxia-inducible factor 1-alpha (HIF-1α) these conditions. Specific interventions within hypoxia–HIF-1α–NLRP3–IL-1β axis in...

Innate immune cells play important roles in tissue injury and repair following acute myocardial infarction (MI). Although reprogramming of macrophage metabolism has been observed during inflammation resolution phases, the mechanistic link to phenotype is not fully understood. In this study, we found that myeloid-specific deletion (mKO) mitochondrial complex I protein, encoded by Ndufs4, reproduced proinflammatory metabolic profile macrophages exaggerated response LPS. Moreover, mKO mice...

The involvement of excitatory N-methyl-D-aspartate (NMDA) receptors in the hypothalamic control pituitary LH secretion is well recognized. Recent evidence shows that nitric oxide (NO), a free radical gas, may act as neurotransmitter brain, and its efflux stimulated by activation NMDA receptors. Studies were undertaken to determine whether NO involved release LHRH surge induced progesterone (P) estrogen-primed ovariectomized rats. Rats 2 weeks later received estradiol benzoate (30 micrograms...

The adult reproductive axis is driven by an intermittent discharge of gonadotropin-releasing hormone (GnRH) generated a network hypothalamic neurons known as the GnRH pulse generator. Although this signal generator operational in infant primates, puberty these species delayed activation shortly after birth central neural mechanism that holds release check during juvenile development. Here, we show that, male rhesus monkey, postnatal pattern activity inversely related to neuropeptide Y (NPY)...

Venous thromboembolism (VTE), the third leading cardiovascular complication, requires more understanding at molecular levels. Here, we have identified miR-145 as a key molecule for regulating thrombus formation in venous thrombosis (VT) employing network based bioinformatics approach and vivo experiments. Levels of showed an inverse correlation with load determined by coagulation variables. MiRNA target prediction tools vitro study tissue factor (TF) gene miR-145. The restoration levels...

Journal Article Neuropeptide Y release from the paraventricular nucleus increases in association with hyperphagia streptozotocin-induced diabetic rats Get access A Sahu, Sahu 1Department of Obstetrics and Gynecology, University Florida College Medicine, Gainesville 32610. Search for other works by this author on: Oxford Academic Google Scholar C Sninsky, Sninsky P Phelps, Phelps M G Dube, Dube S Kalra, Kalra Endocrinology, Volume 131, Issue 6, 1 December 1992, Pages 2979–2985,...

Abstract Leptin regulates food intake and body weight by acting primarily in the hypothalamus. In humans rodents, obesity is associated with hyperleptinaemia, suggesting a possible state of leptin resistance. Thus, to begin examine mechanisms resistance, we developed rat model which chronic central infusion results development resistance leptin's satiety action. Adult male rats were infused chronically into lateral cerebroventricle (160 ng/h) or phosphate‐buffered saline via Alzet pumps for...

Purpose The purpose of this study is to assess the role higher education institutions (HEIs) and their libraries in promoting United Nations’ Sustainable Development Goals (SDGs). For this, a comprehensive bibliometric analysis altmetric score comparison will be conducted on paper published SD, specifically librarianship field. Design/methodology/approach A literature review was using Web Science (WoS) database. Bibliometric indicators were used analyze publications. search strings included...

Journal Article Role of galanin in stimulation pituitary luteinizing hormone secretion as revealed by a specific receptor antagonist, galantide Get access A Sahu, Sahu 1Department Neuroscience, University Florida College Medicine, Gainesville 32610. Search for other works this author on: Oxford Academic Google Scholar B Xu, Xu S P Kalra Endocrinology, Volume 134, Issue 2, 1 February 1994, Pages 529–536, https://doi.org/10.1210/endo.134.2.7507825 Published: 01 1994

Neuropeptide Y (NPY) readily stimulates the release of hypothalamic LHRH and pituitary LH in intact gonadal steroid-primed gonadectomized rats. We have now tested hypothesis that synthesis NPY may be regulated by steroids. To measure effects hormones on release, a permanent push-pull cannula was implanted anterior (AP) sham castrated (controls) or (CAST) male rats, 1 week later, AP perfused with artificial cerebrospinal fluid over 3-4 h period. concentrations perfusates collected at 10-min...

Intracerebroventricular injection of neuropeptide Y (NPY) stimulates a robust dose-related feeding response in the rat. Experimental evidence attests to view that release NPY paraventricular nucleus (PVN), site richly innervated by immunopositive fibers, is responsible for stimulation behavior. However, there little information on neuroendocrine factors involved regulation release, part due unavailability reliable techniques monitor PVN release. In this study, we have validated an vitro...

Venous thromboembolism (VTE), a multi-factorial disease, is the third most common cardiovascular disease. Established genetic and acquired risk factors are responsible for onset of VTE. High altitude (HA) also poses as an additional factor, predisposing individuals to VTE; however, its molecular mechanism remains elusive. This study aimed identify genes/pathways associated with pathophysiology deep vein thrombosis (DVT) at HA. Gene expression profiling DVT patients, who developed either sea...

High-altitude (HA) hypoxia exposure is believed to induce venous thromboembolism (VTE) in otherwise healthy individuals, although this needs be fully established. The present study aims ascertain the role of HA aggravating any predisposition toward VTE and explore whether etiology HA-induced different from that closer sea level. We compared manifestation-matched male patients (HAPs) plains level (SLPs) for 54 parameters, including coagulation-related, fibrinolytic, thrombophilic variables,...

Journal Article Hypothalamic neuropeptide-Y gene expression increases before the onset of ovarian steroid-induced luteinizing hormone surge Get access A Sahu, Sahu 1Department Neuroscience, University Florida College Medicine, Gainesville 32610. Search for other works by this author on: Oxford Academic Google Scholar W R Crowley, Crowley S P Kalra Endocrinology, Volume 134, Issue 3, 1 March 1994, Pages 1018–1022, https://doi.org/10.1210/endo.134.3.8119137 Published: 01 1994

We tested the hypothesis that hyperphagia observed in streptozotocin (STZ)-induced diabetic rats is due to increased release of neuropeptide Y (NPY) paraventricular nucleus (PVN) hypothalamus. In first experiment, male were injected with STZ or vehicle (control) via tail vein and 18-20 days later, NPY levels seven hypothalamic sites vitro from selected evaluated. The results showed association STZ-produced marked hyperglycemia hyperphagia, concentrations four sites, including PVN. Evaluation...

Tonic gonadotrophin secretion throughout the menstrual cycle is regulated by negative-feedback actions of ovarian oestradiol (E₂) and progesterone. Although kisspeptin neurones in arcuate nucleus (ARC) hypothalamus appear to play a major role mediating these feedback steroids nonprimate species, this issue has been less well studied monkey. In present study, we used immunohistochemistry situ hybridisation examine KISS1 expression, respectively, mediobasal (MBH) adult ovariectomised (OVX)...

The testicular regulation of luteinizing hormone (LH) secretion in the adult rhesus monkey is mediated by an indirect action testosterone to decelerate pulsatile gonadotrophin releasing (GnRH) release. Whether this negative feedback involves GnRH gene expression unknown. Therefore, effect bilateral orchidectomy on hypothalamic levels mRNA encoding hypophysiotropic factor was examined. generally considered be through non‐GnRH cells, and present experiment provided opportunity also examine...

In this study, a specific galanin (GAL) receptor antagonist, galantide, was employed to evaluate the role of endogenous GAL in episodic basal and phasic LH release rats. To assess specificity series experiments performed. first experiment, we observed that administration (0.62 nm) intracerebroventricularly (icv) ovarian steroid-primed ovariectomized (ovx) rats rapidly increased plasma levels between 10-30 min, prior injection galantide icv (5 blocked GAL-induced release. second inhibited...

The tachykinins are a group of structurally related peptides found in the rat hypothalamus and anterior pituitary. We have evaluated effects four on LH release male rats. In intact rats, intracerebroventricular (icv) injection neurokinin A (NKA), neuropeptide K (NPK), neuropeptide-gamma (NP gamma) elicited dose-related, transient increases plasma LH. Substance P (SP) was ineffective under these conditions. further examination showed that vitro incubation with either NPK or NP gamma...

Tachykinins, a family of biologically active related peptides, are found in variable amounts the rat hypothalamus. We assessed effects five tachykinins, substance P (SP), neurokinin A (NKA), neuropeptide K (NPK), gamma (NP gamma), and B (NKB), on LH release different experimental model systems ovariectomized rats. In first series experiments rats were implanted with permanent cannulae third cerebroventricle brain. Two weeks later, intracerebroventricular injection 0.5 or 1.25 nm various...