A5101957835- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Hematopoietic Stem Cell Transplantation
- Hematological disorders and diagnostics
- Chronic Myeloid Leukemia Treatments
- Circular RNAs in diseases
- RNA Research and Splicing
- Acute Lymphoblastic Leukemia research
- RNA modifications and cancer
- Cancer therapeutics and mechanisms
- Cytokine Signaling Pathways and Interactions
- Multiple Myeloma Research and Treatments
- Retinoids in leukemia and cellular processes
- Cancer, Hypoxia, and Metabolism
- Lymphoma Diagnosis and Treatment
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Drug Transport and Resistance Mechanisms
- Immune cells in cancer
Henan University Huaihe Hospital and Huaihe Clinical Institute
2010-2024
Kaifeng University
2019
Multiple myeloma (MM) is the second most common hematological cancer all over world. Long non-coding RNA (lncRNA) colon associated transcript-1 (CCAT1) has been reported to play important roles in development and progression of multiple human malignancies. However, little known about its functional role molecular mechanism MM. The aim this study was investigate clinical biological significance CCAT1 Our data showed that relative expression levels were significantly upregulated MM tissues...
Drug resistance remains a major cause of relapse and therapeutic failure in acute myeloid leukemia (AML). Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been documented to act as an oncogene is frequently highly expressed human cancers including AML. However, the function molecular mechanism MALAT1 regulating cytarabine (Ara-C) AML are largely unknown. The expressions miR-96 patients healthy controls were examined by qRT-PCR. CCK-8 flow cytometry assay performed assess...
Deoxyshikonin was reported to exhibit an anti-tumor effect in colorectal cancer. However, no studies are available illustrate the of deoxyshikonin on acute myeloid leukemia (AML). The effects viability, apoptosis, caspase-3/7 activity, and cytochrome (Cyt) C expression were evaluated by Cell Counting Kit-8 assay, flow cytometry analysis, activity western blot respectively. Glucose consumption lactate production measured determine glycolysis level. pyruvate kinase M2 (PKM2) detected...
DOCK family proteins are evolutionarily conserved guanine nucleotide exchange factors for Rho GTPase with different cellular functions. It has been demonstrated that DOCK1 had adverse prognostic effect in acute myeloid leukemia (AML). We first analyzed data of 85 AML patients who were treated chemotherapy and available to DOCK11 expression information found DOCK2 significance AML. In view the known prognosis AML, we then explored role DOCK2. One hundred fifty-six extracted from The Cancer...
It has been demonstrated that microRNA-98 (miR-98) is dysregulated in multiple types of solid tumors, but its expression and impact acute myeloid leukemia (AML) unclear. To explore the prognostic role miR-98 AML, 164 AML patients with data were extracted from The Cancer Genome Atlas (TCGA) database enrolled this study. First, divided into chemotherapy-only (chemotherapy) group allogeneic hematopoietic stem cell transplant (allo-HSCT) group. Each was then two groups by median level miR-98. In...
LncRNAs have been shown to be involved in the biological and pathological processes of acute myeloid leukemia (AML). Hox antisense intergenic RNA 1 (HOTAIRM1) was reported highly expressed AML. However, detailed role molecular mechanism HOTAIRM1 AML pathogenesis remain undefined. In present study, miR-148b expressions patients healthy controls were detected by qRT-PCR. Cell proliferation apoptosis evaluated CCK-8 flow cytometry assays, respectively. The regulatory interaction between...
Taurine-upregulated gene 1 (TUG1) has been reported as an oncogenic long non-coding RNA (lncRNA) in acute myeloid leukemia (AML). Nevertheless, the roles and molecular mechanism of TUG1 drug resistance AML cells are still unclear. Glycolysis level was evaluated by detecting glucose consumption lactate production. qRT-PCR Western blot were performed to detect TUG1, hexokinase2 (HK2) pyruvate kinase isoenzyme M2 (PKM2) expressions. Adriamycin (ADR) cytotoxicity apoptosis assessed MTT assay...
Abstract Objectives This study retrospectively investigated in which cycle measurable residual disease (MRD) is associated with prognosis patients first complete remission (CR1) of intermediate-risk acute myeloid leukemia (AML). Methods The enrolled 235 younger AML. MRD was evaluated by multiparameter flow cytometry after the 1st, 2nd, and 3rd chemotherapy cycles (MRD1–3, respectively). Results No significant association detected 1st 2nd cycles. However, 5-year incidence relapse higher...
Adriamycin (ADR) is a widely used drug in multiple cancers including leukemia. Artesunate (ART) has been reported to improve the cytotoxicity of some chemotherapeutic drugs cancers. However, it still unknown whether ART can augment ADR ADR-resistant K562 leukemia cells (K562/ADR). Glycolytic activity was assessed by detecting glucose consumption, lactate production and glycolysis-related enzymes. MDR1 ABCG2 mRNA levels were measured RT-qPCR. Protein P-glycoprotein (P-gp), cytochrome c (Cyt...
Abstract The purpose of the present study was to investigate whether expression levels adenylate kinase 1 (AK1) were associated with prognosis acute myeloid leukemia (AML) in patients treated chemotherapy or allogeneic hematopoietic stem cell transplantation (allo-HSCT). 85 AML AK1 report who received chemotherapy-alone and 71 underwent allo-HSCT from Cancer Genome Atlas database identified grouped into either AK1high AK1low based on their level relative median. Then, overall survival (OS)...
Background: Interferon-α-1b, interleukin-2 combined with thalidomide (ITI) improved the outcome and prognosis of some acute myeloid leukemia (AML) patients, but cases was insufficient. This study observed efficacy safety this regimen in treatment numbers AML patients various disease states. Methods: Starting January 2014, (n=188) were treated ITI regimen, including 60 refractory/relapses group A, 40 B remained minimal residual disease-positive (MRD) or changed from negative to positive again...
Background/Aims: Acute myeloid leukemia (AML) of French-American-British (FAB) subtypes M0 and M1 are both poorly differentiated AML, but their mutational spectrum molecular characteristics remain unknown. This study aimed to explore the prognostic factors AML-M0 M1. Methods: Sixty-five AML patients derived from The Cancer Genome Atlas (TCGA) database were enrolled in this study. Whole-genome sequencing was performed depict each patient. Clinical at diagnosis, including peripheral blood (PB)...
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is considered the cure for intermediate or adverse-risk acute myeloid leukemia (AML) (1). It offers patients longer overall survival (OS) (2-4). Unfortunately, human leukocyte antigen (HLA) matched related donor unavailable in more than 70% patients. Some of these patients, may experience relapse and subsequently achieve CR (non-CR1) with treatment. non-CR1 eventually receive allo-HSCT. unclear whether allo-HSCT will improve
Abstract Background: Risk stratification for normal karyotype acute myeloid leukemia remains unsatisfactory, which is reflected by the high incidence of relapse. This study aimed to evaluate role gene mutations and clinical characterization in predicting relapse patients with leukemia. Methods: A prognostic system was constructed based on mutations, measurable residual disease, characteristics. panel explored using next-generation sequencing. The least absolute shrinkage selection operator,...
Risk stratification for normal karyotype acute myeloid leukemia (NK-AML) remains unsatisfactory, which is reflected by the high incidence of relapse. This study aimed to evaluate role gene mutations and clinical characterization in predicting relapse patients with NK-AML. A prognostic system NK-AML was constructed. panel explored using next-generation sequencing. nomogram algorithm used build a genomic mutation signature (GMS) (GMSN) model that combines GMS, measurable residual disease,...
To explore the effect of hypoxia on cell proliferation and expression hypoxia-inducible factor-1α(HIF-1α) human leukemia K562 cells.The cells were divided into 2 groups: hypoxia-treated group conventional oxygen as control. The in treated with 50, 200, 400 800 µmol/L CoCl2, while control cultured condition, then groups colleted after treatment for 24, 48 72 hours. morphological changes observed by inverted phase-contrast microscopy, proliferation-inhibitory rates was detected MTT method,...
To investigate the application value of immunohistochemistry detection and bone marrow morphology analysis in diagnosis clinical staging non-Hodgkin's lymphoma.Sixty-four cases lymphoma were selected our hospital pathologic specimens was perforemed by related monoclonal antibody also examined.The positive rate CD79a on B cell 84.62%, CD20 91.43%; CD45RO T 87.50%, 88.89% CD3. The involvement found 10 (15.63%), 8 cases(12.50%) progressed to - leukemia period; cells lower than 5% 20...