A5054962431- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- ATP Synthase and ATPases Research
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Endoplasmic Reticulum Stress and Disease
- Histone Deacetylase Inhibitors Research
- Thyroid Disorders and Treatments
- Cannabis and Cannabinoid Research
- Protein Degradation and Inhibitors
- Protein Hydrolysis and Bioactive Peptides
- Muscle Physiology and Disorders
- Genetic Neurodegenerative Diseases
- Growth Hormone and Insulin-like Growth Factors
- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Parkinson's Disease Mechanisms and Treatments
- Pancreatic function and diabetes
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
Instituto de Investigación Biomédica de Málaga
2020-2021
Universidad de Málaga
2001-2021
Salk Institute for Biological Studies
2010-2014
Institute for Research in Biomedicine
2008-2013
Universitat de Barcelona
2004-2013
Instituto de Salud Carlos III
2012-2013
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2008-2013
Mitochondria are dynamic organelles that play a key role in energy conversion. Optimal mitochondrial function is ensured by quality-control system tightly coupled to fusion and fission. In this connection, mitofusin 2 (Mfn2) participates undergoes repression muscle from obese or type diabetic patients. Here, we provide vivo evidence Mfn2 plays an essential metabolic homeostasis. Liver-specific ablation of mice led numerous abnormalities, characterized glucose intolerance enhanced hepatic...
Background There is no evidence to date on whether transcriptional regulators are able shift the balance between mitochondrial fusion and fission events through selective control of gene expression. Methodology/Principal Findings Here, we demonstrate that reduced size observed in knock-out mice for regulator PGC-1β associated with a reduction Mitofusin 2 (Mfn2) expression, protein. This decrease Mfn2 specific since expression remaining components machinery were not affected. Furthermore,...
Glutaminase (GA) catalyzes the first step in mitochondrial glutaminolysis playing a key role cancer metabolic reprogramming. Humans express two types of GA isoforms: GLS and GLS2. isozymes have been consistently related to cell proliferation, but GLS2 remains poorly understood. is repressed many tumor cells better understanding its function tumorigenesis may further development new therapeutic approaches. We analyzed expression HCC, GBM neuroblastoma cells, as well monkey COS-7 cells....
Neuregulins are growth factors that essential for myogenesis and regulate muscle metabolism. The addition of a recombinant neuregulin-1 isoform, heregulin-beta1(177-244) (Hrg), containing 3 nmol/l the bioactive epidermal factor-like domain, to developing L6E9 myocytes has acute chronic effects on glucose uptake enhances myogenesis. Here, we studied metabolic adaptation treatments with Hrg.L6E9 C2C12 were chronically treated low concentrations Hrg (3 pmol/l) do not induce We analyzed cellular...
Diabetes is characterized by reduced thyroid function and altered myogenesis after muscle injury. Here we identify a novel component of hormone action that repressed in diabetic rat muscle.We have identified gene, named DOR, abundantly expressed insulin-sensitive tissues such as skeletal heart, whose expression highly from obese rats. DOR up-regulated during differentiation its loss-of-function has negative impact on gene programmes linked to or driven hormones. In agreement with this,...
Glucocorticoids, such as dexamethasone, enhance hepatic energy metabolism and gluconeogenesis partly through changes in mitochondrial function. Mitochondrial function is influenced by the balance between fusion fission events. However, whether glucocorticoids modulate regulation of dynamics currently unknown.Here, we report that effects dexamethasone on hepatoma cells are dependent protein dynamin-related 1 (Drp1). Dexamethasone increased routine oxygen consumption, maximal respiratory...
Myotonic dystrophy (DM) is caused by a CTG expansion in the 3'-untranslated region of protein kinase gene (DMPK). Cardiovascular disease one most prevalent causes death DM patients. Electrophysiological studies cardiac muscles from patients and DMPK(-/-) mice suggested that DMPK critical to modulation contractility maintenance proper conduction activity. However, there are no data regarding molecular signaling pathways involved heart failure. Here we show expression myocytes highly enriched...
Mitofusin 2 (Mfn2), a protein that participates in mitochondrial fusion, is required to maintain normal metabolism skeletal muscle and liver. Given Mfn2 repressed obese or type diabetic subjects, this may have potential pathophysiological role these conditions. To evaluate whether the metabolic effects of can be dissociated from its function dynamics, we studied form human Mfn2, lacking two transmembrane domains COOH-terminal coiled coil (ΔMfn2). This localized mitochondria but did not alter...
Significance Catecholamines regulate adipocyte function in part by CREB activation. Obesity displays enhanced cyclic AMP response element binding protein (CREB) activity despite reduced catecholamine signals. We report that obesity promotes (CBP)-mediated acetylation at Lys136 the adipose tissue means of SirT1 levels. with bromodomain (BRD) CBP, which can also bind phosphorylated Ser133, through its KIX domain, upon hormonal This double recognition is reflected potentiated CBP recruitment...
The effects of histamine on polyamine uptake and metabolism was studied in a mouse mast cell line (C57.1), as model which both biogenic amines are important for maintaining function viability. Results obtained after incubations with exogenous indicated that prevents accumulation by affecting uptake. A plasma membrane transport system polyamines has been also cells. It seems to be Na(+)-dependent high affinity spermine spermidine lower putrescine agmatine. Polyamine reduced cells treated...
An amendment to this paper has been published and can be accessed via a link at the top of paper.