A5063885848- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Advanced Fluorescence Microscopy Techniques
- Advanced Drug Delivery Systems
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
- Optical Coherence Tomography Applications
- Field-Flow Fractionation Techniques
- Bacteriophages and microbial interactions
- Advancements in Transdermal Drug Delivery
- Neonatal Respiratory Health Research
- Viral gastroenteritis research and epidemiology
- Advanced Electron Microscopy Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Hernia repair and management
- Mechanical and Optical Resonators
- Acoustic Wave Resonator Technologies
- Diffusion and Search Dynamics
- Antimicrobial Peptides and Activities
- Graphene and Nanomaterials Applications
- Intraperitoneal and Appendiceal Malignancies
- Ocular Surface and Contact Lens
- Ultrasound and Cavitation Phenomena
- Ultrasound and Hyperthermia Applications
Ghent University
2014-2018
University College London
2016
Università Cattolica del Sacro Cuore
2012
A design for photoacoustic mass sensors operating above 100 GHz is proposed. The based on impulsive optical excitation of a pseudosurface acoustic wave in surface phononic crystal with nanometric periodic grating and time-resolved extreme ultraviolet detection the frequency shift upon loading device. present opens path to range currently unaccessible electro-acoustical transducers, providing enhanced sensitivity, miniaturization, incorporating time-resolving capability while forgoing...
Light sheet microscopy is a relatively new form of fluorescence that has been receiving lot attention recently. The strong points the technique, such as high signal to noise ratio and its reduced photodamage fluorescently labelled samples, come from unique feature illuminate only thin plane in sample coincides with focal detection lens. Typically this requires two closely positioned perpendicular objective lenses, one for illumination. Apart fact special configuration lenses incompatible...
Cell-penetrating peptides (CPPs) are efficient vehicles to transport bioactive molecules into the cells. Despite numerous studies exact mechanism by which CPPs facilitate delivery of cargo its intracellular target is still debated. The current work presents methods that can be used for tracking CPP/pDNA complexes through endosomal and show role in cargo. Separation vesicles differential centrifugation enables pinpoint localization delivered without labeling it gives important quantitative...
The development of biotechnological pharmaceutics, like macro- and nanocarriers, can benefit greatly from studying their characteristics in situ using advanced fluorescence microscopy methods. While choosing the optimal labeling method for visualizing carrier or its cargo is crucial, it seldom receives attention. possibility that high densities alter intracellular processing molecule considered, but how at which point this interference happens not yet studied. aim study was to elucidate...
We present an approximate Bayesian computation scheme for estimating number concentrations of monodisperse diffusing nanoparticles in suspension by optical particle tracking microscopy. The method is based on the probability distribution time spent a inside detection region. validate suspensions well-controlled reference particles. illustrate its usefulness with application gene therapy, applying to estimate plasmid DNA molecules and average complexed liposomal drug delivery
Nucleic acid biopharmaceuticals are being investigated as potential therapeutics. They need to be incorporated into a biocompatible carrier so overcome several biological barriers. Rational development of suitable nanocarriers requires high-quality characterization techniques. While size, concentration, and stability can very well measured these days, even in complex fluids, method accurately quantify the number nucleic therapeutics encapsulated is still missing. Here we present method,...