A5043649415- Bacterial Identification and Susceptibility Testing
- Legionella and Acanthamoeba research
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Sepsis Diagnosis and Treatment
- Respiratory viral infections research
- Erythrocyte Function and Pathophysiology
- Heme Oxygenase-1 and Carbon Monoxide
- Mycobacterium research and diagnosis
- Meta-analysis and systematic reviews
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Clinical Laboratory Practices and Quality Control
- Biomedical Ethics and Regulation
- Biosensors and Analytical Detection
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antifungal resistance and susceptibility
- Toxoplasma gondii Research Studies
- COVID-19 diagnosis using AI
- Data-Driven Disease Surveillance
- Antibiotic Use and Resistance
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Diphtheria, Corynebacterium, and Tetanus
- Diet and metabolism studies
OpGen (United States)
2016-2019
Banner - University Medical Center Tucson
2013-2016
University of Arizona
2015
University of Michigan
2006-2014
To restrict infection by Legionella pneumophila, mouse macrophages require Naip5, a member of the nucleotide-binding oligomerization domain leucine-rich repeat family pattern recognition receptors, which detect cytoplasmic microbial products. We report that restricted L. pneumophila replication and initiated proinflammatory program cell death when flagellin contaminated their cytosol. Nuclear condensation, membrane permeability, interleukin-1β secretion were triggered type IV...
Rapid identification from positive blood cultures is standard of care (SOC) in many clinical microbiology laboratories. The GenMark Dx ePlex Blood Culture Identification Gram-Positive (BCID-GP) Panel a multiplex nucleic acid amplification assay based on competitive DNA hybridization and electrochemical detection using eSensor technology. This multicenter study compared the investigational-use-only (IUO) BCID-GP to other methods 20 Gram-positive bacteria, four antimicrobial resistance genes,...
Understanding bacterial species at highest risk for harboring bla CTX-M genes is necessary to guide antibiotic treatment. We identified the species-specific prevalence of in clinical isolates from United States.
Routine identification of fungal pathogens from positive blood cultures by culture-based methods can be time-consuming, delaying treatment with appropriate antifungal agents. The GenMark Dx ePlex investigational use only culture pathogen panel (BCID-FP) rapidly detects 15 targets simultaneously in samples for fungi Gram staining. We aimed to determine the performance BCID-FP a multicenter clinical study. Blood collected at 10 United States sites and tested 4 were compared standard-of-care...
Background The B inational order I nfectious D isease S urveillance program began surveillance for severe acute respiratory infections ( SARI ) on the US –Mexico border in 2009. Here, we describe patients outhern A rizona. Methods Patients admitted to five care hospitals that met case definition (temperature ≥37·8°C or reported fever chills with history of cough, sore throat, shortness breath a hospitalized person) were enrolled. Staff completed standard form and collected nasopharyngeal...
Bacteremia can progress to septic shock and death without appropriate medical intervention. Increasing evidence supports the role of molecular diagnostic panels in reducing clinical impact these infections through rapid identification infecting organism associated antimicrobial resistance genes. We report results a multicenter study assessing performance GenMark Dx ePlex investigational-use-only blood culture Gram-negative panel (BCID-GN), assay for detection bloodstream pathogens positive...
We developed a rapid high-throughput PCR test and evaluated highly antibiotic-resistant clinical isolates of Escherichia coli ( n = 2,919), Klebsiella pneumoniae 1,974), Proteus mirabilis 1,150), Pseudomonas aeruginosa 1,484) for several antibiotic resistance genes comparison with phenotypic across penicillins, cephalosporins, carbapenems, aminoglycosides, trimethoprim-sulfamethoxazole, fluoroquinolones, macrolides. The originated from hospitals in North America (34%), Europe (23%), Asia...
<title>Abstract</title> Many patients in the emergency department present with signs and symptoms that arouse concern for sepsis; however, other explanations are also possible. There currently no rapid tests used clinical practice reliably distinguish presence of a bacterial or viral infection vs. non-infectious etiology can predict patient’s likelihood to decompensate. The diagnostic prognostic uncertainty “gray zone” complicates decision begin therapy as clinicians need balance risk...
Legionella pneumophila, a motile opportunistic pathogen of humans, is restricted from replicating in the lungs C57BL/6 mice. Resistance mouse macrophages to L. pneumophila depends on recognition cytosolic flagellin. Once detected by NOD-like receptors Naip5 and Ipaf (Nlrc4), flagellin triggers pyroptosis, proinflammatory cell death. In contrast, strains parisiensis tucsonensis replicate profusely within macrophages, similar flagellin-deficient pneumophila. To gain insight into how species...
Infections are common complications in patients following liver transplantation (LTX). The early diagnosis and prognosis of these infections is an unmet medical need even when using routine biomarkers such as C-reactive protein (CRP) procalcitonin (PCT). Therefore, new approaches necessary.
To restrict infection by Legionella pneumophila, mouse macrophages require Naip5, a member of the nucleotide-binding oligomerization domain leucine-rich repeat family pattern recognition receptors, which detect cytoplasmic microbial products.We report that restricted L. pneumophila replication and initiated proinfl ammatory program cell death when fl agellin contaminated their cytosol.Nuclear condensation, membrane permeability, interleukin-1 secretion were triggered type IV...
This presentation will cover the enhancement of Severe Acute Respiratory Surveillance in Arizona-Mexico Border region from 2010 through present. It discuss process establishing surveillance, comparibility between countries data, analysis, and lessons learned.