A5050722758- Cancer Immunotherapy and Biomarkers
- Colorectal Cancer Treatments and Studies
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- interferon and immune responses
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Sex and Gender in Healthcare
- Cancer, Stress, Anesthesia, and Immune Response
- Receptor Mechanisms and Signaling
- Chemokine receptors and signaling
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Cancer Research and Treatments
- Immune cells in cancer
- Toxin Mechanisms and Immunotoxins
- Melanoma and MAPK Pathways
- Cardiac Ischemia and Reperfusion
- Monoclonal and Polyclonal Antibodies Research
- Nanoparticle-Based Drug Delivery
- Angiogenesis and VEGF in Cancer
- Nanoplatforms for cancer theranostics
- SARS-CoV-2 and COVID-19 Research
- Neuropeptides and Animal Physiology
Istituto Superiore di Sanità
2012-2022
Information Technology Association of Canada
2022
Molecular Oncology (United States)
2016
Karolinska Institutet
2014
Acidic pH is an important feature of tumor microenvironment and a major determinant progression. We reported that cancer cells upregulate autophagy as survival mechanism to acidic stress. Inhibition by administration chloroquine (CQ) in combination anticancer therapies currently evaluated clinical trials. observed 3 different human cell lines cultured at autophagic flux not blocked CQ. This was consistent with complete resistance CQ toxicity conditions. Conversely, the autophagy-inhibiting...
Immunotherapy efficacy relies on the crosstalk within tumor microenvironment between cancer and dendritic cells (DCs) resulting in induction of a potent effective antitumor response. DCs have specific role recognizing cells, taking up antigens (Ags) then migrating to lymph nodes for Ag (cross)-presentation naïve T cells. Interferon-α-conditioned (IFN-DCs) exhibit marked phagocytic activity special ability inducing Ag-specific T-cell Here, we developed novel microfluidic platform recreating...
Abstract Background Inefficient T-cell access to the tumor microenvironment (TME) is among causes of immune-resistance. Previous evidence demonstrated that targeting CXCR4 improves anti-PD-1/PD-L1 efficacy reshaping TME. To evaluate role newly developed antagonists (PCT/IB2011/000120/ EP2528936B1/US2013/0079292A1) in potentiating anti-PD-1 two syngeneic murine models, MC38 colon cancer and B16 melanoma-human CXCR4-transduced, were employed. Methods Mice subcutaneously injected with (1 × 10 6...
// Sara Santagata 1 , Maria Napolitano Crescenzo D'Alterio Sonia Desicato 2 Salvatore Di Maro 3 Luciana Marinelli Alessandra Fragale 4 Buoncervello Francesco Persico 5 Lucia Gabriele Ettore Novellino Nicola Longo Sandro Pignata Sisto Perdonà and Stefania Scala Functional Genomics, Istituto Nazionale per lo Studio e la Cura dei Tumori, Fondazione “G. Pascale”-IRCCS, 80131 Naples, Italy Uro-Gynecological Department, Department of Pharmacy, University Naples Federico II,...
Background and Purpose Propranolol is a vasoactive drug that shows antiangiogenic antitumour activities in melanoma. However, it unknown whether these are dose‐dependent there relationship between systemic vascular effects of propranolol anti‐melanoma activity. Experimental Approach Effects increasing doses (10, 20, 30 40 mg·kg −1 ·day ) on tumour growth were studied B16F10 melanoma‐bearing mice. Histological biochemical analyses used to assess angiogenesis cancer cell proliferation....
Abstract Cardiac dysfunction is often observed in patients with cancer also representing a serious problem limiting chemotherapeutic intervention and even patient survival. In view of the recently established role immune system control growth, present work has been undertaken to investigate effects panel most important inflammatory cytokines on integrity function mitochondria, as well cytoskeleton, two key elements functioning cardiomyocytes. Either mitochondria features or actomyosin...
// Paola Pellegrini 1 , Matheus Dyczynski Francesca Vittoria Sbrana 2 Maria Karlgren 3 Buoncervello 4 Hägg-Olofsson Ran Ma Johan Hartman Svetlana Bajalica-Lagercrantz Dan Grander Pedram Kharaziha and Angelo De Milito Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska Institute, Stockholm, Sweden Istituto Ortopedico Rizzoli, Bologna, Italy Pharmacy Uppsala University Drug Optimization Pharmaceutical Profiling Platform (UDOPP) - Science for Life Laboratory, Pharmacy,...
Currently approved combination regimens available for the treatment of metastatic tumors, such as breast cancer, have been shown to increase response rates, often at cost a substantial in toxicity. An ideal strategy may consist agents with different mechanisms action leading complementary antitumor activities and safety profiles. In present study, we investigated effects epigenetic modulator apicidin cytotoxic agent docetaxel tumor cell lines characterized by grades invasiveness. We report...
// Maria Buoncervello 1 , Giulia Romagnoli 1, * Mariachiara Buccarelli Alessandra Fragale Elena Toschi Stefania Parlato Donatella Lucchetti 2 Daniele Macchia Massimo Spada Irene Canini Sanchez 3 Mario Falchi 4 Martina Musella Mauro Biffoni Filippo Belardelli Imerio Capone Alessandro Sgambato Lucia Ricci Vitiani Gabriele Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy Patologia Generale, Università Cattolica del Sacro Cuore,...
Colorectal cancer results from the progressive accumulation of genetic and epigenetic alterations. IFN signaling defects play an important role in carcinogenesis process, which inability transcription regulatory factors (IRF) to access sequences IFN-stimulated genes (ISG) tumors immune cells may be pivotal. We reported that low-dose combination two FDA-approved epidrugs, azacytidine (A) romidepsin (R), with IFNα2 (ARI) hampers aggressiveness both colorectal metastatic stem vivo triggers...
The antimalarial drug Pyrimethamine has been suggested to exert an antitumor activity by inducing apoptotic cell death in cancer cells, including metastatic melanoma cells. However, the dose of be considered as anticancer agent appears significantly higher than maximum used antiprotozoal drug. Hence, a series analogs synthesized and screened for their apoptosis induction two cultured lines. One these analogs, Methylbenzoprim, was further analyzed evaluate cell-cycle mechanisms death. effects...
PL-Peptide R potentiates the Peptide efficacy and efficiently delivers doxorubicin in melanoma lung metastasis.
Antagonizing the oncogenic effects of human epidermal growth factor receptor 2 (HER2) with current anti-HER2 agents has not yet yielded major progress in treatment advanced HER2-positive epithelial ovarian cancer (EOC). Using preclinical models to explore alternative molecular mechanisms affecting HER2 overexpression and oncogenicity may lead new strategies for EOC patient treatment. We previously reported that phosphatidylcholine-specific phospholipase C (PC-PLC) exerts a pivotal role...
Recently, β-adrenoceptor blockade has emerged as a potential strategy to inhibit melanoma growth. It remains be ascertained whether stimulation by circulating catecholamines increases growth in mice.B16F10 melanoma-bearing mice were used evaluate effects of adrenaline and specific adrenoceptor (AR) ligands on tumour volume. AR expression cell viability, production mitochondrial reactive oxygen species (mROS), proliferation activity B16F10 cells, determined biochemical analyses.Real-time...
BRAFV600 mutations are the most common oncogenic alterations in melanoma cells, supporting proliferation, invasion, metastasis and immune evasion. In patients, these aberrantly activated cellular pathways inhibited by BRAFi whose potent antitumor effect therapeutic potential dampened development of resistance. Here, using primary cell lines, generated from lymph node lesions metastatic we show that combination two FDA-approved drugs, histone deacetylate inhibitor (HDCAi) romidepsin...
Abstract Introduction. CXCR4 is overexpressed in multiple tumors regulating metastatic dissemination. A new class of cyclic peptides antagonist for receptors was recently developed1. To improve peptide efficacy and increase its delivery to target cancer cells the most active antagonist, Pep R, coupled PEGylated liposomes (PL). Experimental Procedures. PL conjugated (Lip-PepR) were prepared starting by athiolated derivative antiCXCR4 pre-formed PL. Doxorubicin (DOX) then encapsulated remote...
<p>Supplementary Figure 3 Biological processes categories enriched in the list of genes showing H3K9me2 peaks promoter region.</p>
<p>Supplementary Table 1. Sequences of oligonucleotides used in RT-qPCR are listed. Supplementary 2. ChIP assays qPCR.</p>
<div>Abstract<p>Colorectal cancer results from the progressive accumulation of genetic and epigenetic alterations. IFN signaling defects play an important role in carcinogenesis process, which inability transcription regulatory factors (IRF) to access sequences IFN-stimulated genes (ISG) tumors immune cells may be pivotal. We reported that low-dose combination two FDA-approved epidrugs, azacytidine (A) romidepsin (R), with IFNα2 (ARI) hampers aggressiveness both colorectal...
<p>Supplementary Figure 1 contains ten panels of RT-qPCR analisis CRC</p>
<p>Supplementary Figure 4 shows Viability graphs and Sytox staining of IL4-ARI DC IFN-lambda1, IFNLR1, IFN-betaand IFN-panalpha expression by RT-qPCR in DC.</p>