A5005538062- Tuberculosis Research and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- COVID-19 Clinical Research Studies
- Extracellular vesicles in disease
- Long-Term Effects of COVID-19
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Diagnosis and treatment of tuberculosis
- Supramolecular Self-Assembly in Materials
- Immune cells in cancer
- Autophagy in Disease and Therapy
- Monoclonal and Polyclonal Antibodies Research
- Neuroendocrine Tumor Research Advances
- Pancreatic and Hepatic Oncology Research
- vaccines and immunoinformatics approaches
- Influenza Virus Research Studies
- Adenosine and Purinergic Signaling
- Antifungal resistance and susceptibility
- Curcumin's Biomedical Applications
- Bacterial Infections and Vaccines
- Sulfur Compounds in Biology
- Immune Cell Function and Interaction
Rutgers, The State University of New Jersey
2019-2025
Rutgers New Jersey Medical School
2020-2024
Rutgers Sexual and Reproductive Health and Rights
2023
Indo Soviet Friendship College of Pharmacy
2023
Rütgers (Germany)
2023
NIMS University
2022
All India Institute of Medical Sciences
2022
Liaquat University of Medical & Health Sciences
2020-2021
AdventHealth Orlando
2018-2020
Dow University of Health Sciences
2018-2020
Mycobacterium tuberculosis (M.tb.) replicates in host macrophages to cause tuberculosis. We have investigated the role of miRNAs M.tb.-infected murine RAW264.7 cells and bone marrow-derived (BMDMs), focusing on miR-155, most highly upregulated miRNA. observed that miR-155 upregulation is directly linked attenuation expression BTB CNC homology 1 (Bach1) SH2-containing inositol 5'-phosphatase (SHIP1). Bach1 a transcriptional repressor haem oxygenase-1 (HO-1), whereas SHIP1 inhibits activation...
For efficient clearance of Mycobacterium tuberculosis (Mtb), macrophages tilt towards M1 polarization leading to the activation transcription factors associated with production antibacterial effector molecules such as nitric oxide (NO) and proinflammatory cytokines interleukin 1 β (IL-1β) tumor necrosis factor α (TNF-α). At same time, resolution inflammation is M2 increased arginase IL-10. The transcriptional post-transcriptional mechanisms that govern balance between polarization,...
Autophagy plays a crucial role in the control of bacterial burden during Mycobacterium tuberculosis infection. MicroRNAs (miRNAs) are small non-coding RNAs that regulate immune signalling and inflammation response to challenge by pathogens. Appreciating potential host-directed therapies designed autophagy mycobacterial infection, we focused on miRNAs regulating M. tuberculosis-induced macrophages. Here, demonstrate infection leads downregulation miR-17 concomitant upregulation its targets...
The lasting threat of viral pandemics necessitates the development tailorable first-response antivirals with specific but adaptive architectures for treatment novel infections. Here, such an antiviral platform has been developed based on a mixture hetero-peptides self-assembled into functionalized β-sheets capable multivalent binding to protein complexes. One domain each hetero-peptide is designed specifically bind certain proteins, while another self-assembles fibrils epitope...
SARS-CoV-2 has led to a worldwide pandemic, catastrophically impacting public health and the global economy. Herein, new class of lipid-modified polymer poly (β-amino esters) (L-PBAEs) is developed via enzyme-catalyzed esterification further formulation L-PBAEs with poly(d,l-lactide-coglycolide)-
SARS-CoV-2 virions enter the host cells by docking their spike glycoproteins to membrane-bound Angiotensin Converting Enzyme 2. After intracellular assembly, newly formed are released from infected propagate infection, using extra-cytoplasmic ACE2 mechanism. However, molecular events underpinning transmission between not fully understood. Here, we report findings of a scanning Helium-ion microscopy study performed on Vero E6 with mNeonGreen-expressing SARS-CoV-2. Our data reveal,...
Granulomas are an important hallmark of Mycobacterium tuberculosis infection. They organized and dynamic structures created when immune cells assemble around the sites infection in lungs that locally restrict M. growth host's inflammatory responses. The cellular architecture granulomas is traditionally studied by immunofluorescence labeling surface markers on host cells. However, very few Abs available for model animals used research, such as nonhuman primates rabbits, secreted immunological...
Extrapulmonary tuberculosis (EPTB) accounts for approximately 17% of all Mycobacterium (M.tb) infections globally. Immunocompromised individuals, such as those with HIV infection or type 2 diabetes mellitus (T2DM), are at an increased risk EPTB. Previous studies have demonstrated that patients and T2DM exhibit diminished synthesis glutathione (GSH) synthesizing enzymes. In a murine model, we showed the diethyl maleate (DEM)-induced depletion GSH in lungs led to M.tb burden impaired pulmonary...
Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis ( Mtb ), remains a global health challenge. Arginine metabolism is central to immune responses, regulating nitric oxide (NO) production via inducible NO synthase (Nos2) and competing pathways mediated arginases (Arg1 Arg2). This study examines the impact of arginine supplementation arginase inhibition during acute phase infection in mouse lungs, focusing on function, lung pathology, mitochondrial function. enhanced Nos2...
Autophagy is an intracellular catabolic process that required to maintain cellular homeostasis. Pathogen-elicited host cell autophagy may favour containment of infection or help in bacterial survival. Pathogens have developed the ability modulate autophagy. The secreted antigen HP0175, a peptidyl prolyl cis,trans isomerase Helicobacter pylori, has moonlighting functions with reference cells. Here we show it executes gastric epithelial dependent on unfolded protein response (UPR) activates...
The Coronavirus disease 2019 (COVID-19) pandemic disproportionately affects immunocompetent and immunocompromised individuals, with the latter group being more vulnerable to severe death. However, differential pathogenesis of SARS-CoV-2 in context a specific immunological niche remains unknown. Similarly, systematic analysis pathology various extrapulmonary organs hosts during infection is not fully understood. We used hamster model infection, which recapitulates pathophysiology patients...
Mycobacterium tuberculosis (M. tb), the causative agent of (TB), is responsible for causing significant morbidity and mortality, especially among individuals with compromised immune systems. We have previously shown that supplementation liposomal glutathione (L-GSH) reduces M. tb viability enhances a Th-1 cytokine response, promoting granuloma formation in human peripheral blood mononuclear cells vitro. However, effects L-GSH modulating responses lungs during an active infection yet to be...
Despite the availability of antibiotic therapy, tuberculosis (TB) is prevailing as a leading killer among human infectious diseases, which highlights need for better intervention strategies to control TB. Several animal model systems, including mice, guinea pigs, rabbits, and non-human primates have been developed explored understand TB pathogenesis. Although each these models contributes our current understanding host-Mycobacterium (Mtb) interactions, none fully recapitulate pathological...
The pathologic consequences of Coronavirus Disease-2019 (COVID-19) include elevated inflammation and dysregulated vascular functions associated with thrombosis. In general, disruption homeostasis ensuing prothrombotic events are driven by activated platelets, monocytes, macrophages, which form aggregates (thrombi) attached to the endothelium lining vessel walls. However, molecular pathways underpinning pathological interactions between myeloid cells during COVID-19 remain undefined. Here, we...
Abstract In vitro phagocytosis of Mycobacterium tuberculosis (Mtb) aggregates (Mtb-AG), rather than similar numbers single bacilli (Mtb-SC), induces host macrophage death and favors bacterial growth. Here, we examined whether aggregation contributes to enhanced Mtb pathogenicity in vivo rabbit lungs. Rabbits were exposed infectious aerosols containing mainly Mtb-AG or Mtb-SC. The lung load, systemic immune response, histology, cell composition investigated over time. Genome-wide...
Both active tuberculosis (TB) and asymptomatic latent Mycobacterium (M. tb) infection (LTBI) cause significant health burdens to humans worldwide. Individuals with immunocompromising conditions, such as Type 2 Diabetes Mellitus (T2DM), have a weakened ability control M. tb are more susceptible reactivation of LTBI diseases. T2DM cases known glutathione (GSH) deficiency impaired immune cell function, including the granulomatous response infection. We previously reported that liposomal (L-GSH)...
Mycobacterium tuberculosis (M. tb), the causative agent of (TB), continues to be a global health burden. We have reported that patients with marked deficiency in production glutathione (GSH) had impaired granulomatous effector responses against M. tb infection, which were restored when supplementing liposomal GSH (lGSH). However, effects lung parenchyma altering granuloma formation and infection remain unexplored. aim elucidate diethyl maleate (DEM)-induced during an active vivo mouse model....
Tuberculosis (TB) is a public health concern that impacts 10 million people around the world. Current in vitro models are low throughput and/or lack caseation, which impairs drug effectiveness humans. Here, we report generation of THP-1 human monocyte/macrophage spheroids housing mycobacteria (TB spheroids). These TB have central core dead cells co-localized with and hypoxic. exhibit higher levels pro-inflammatory factor TNFα growth factors G-CSF VEGF when compared to non-infected control....
Abstract Macrophages play an important role in the establishment of infection by intracellular pathogens. Mycobacterium tuberculosis is known to inhibit apoptosis and downregulate immune responses host cells using various strategies, including activation peroxisome proliferator‐activated receptor (PPAR)γ. Mannose‐capped lipoarabinomannan (ManLAM) one bacterial effectors that plays a subversion immunity PPARγ. Here, we have used unbiased global gene expression profiling approach understand (...
The Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) causes Coronavirus disease-2019 (COVID-19), which is an ongoing pandemic that has significantly affected the health, economy, and socio-economic status of individuals worldwide. Laboratory research using in vitro, ex vivo models been accelerated to understand pathogenesis SARS-CoV-2 infection. However, such experimental involving restricted biocontainment/safety level-3 (BSL-3) settings, due high pathogenicity this virus. Since...