A5048266715- Complement system in diseases
- MicroRNA in disease regulation
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Cancer Mechanisms and Therapy
- Cytokine Signaling Pathways and Interactions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Tuberculosis Research and Epidemiology
- Iron Metabolism and Disorders
- Antibiotic Resistance in Bacteria
- Extracellular vesicles in disease
- RNA modifications and cancer
- Pneumonia and Respiratory Infections
- Circular RNAs in diseases
- Optical and Acousto-Optic Technologies
- Burkholderia infections and melioidosis
- Viral Infections and Vectors
- Blood Coagulation and Thrombosis Mechanisms
- Plasmonic and Surface Plasmon Research
- Phagocytosis and Immune Regulation
- Platelet Disorders and Treatments
- COVID-19 Impact on Reproduction
- Autophagy in Disease and Therapy
Washington University in St. Louis
2021-2024
Bose Institute
2012-2024
Pulmonary and Critical Care Associates
2021
Lovely Professional University
2020
Indian Institute of Technology Bhilai
2019
Complement activation has been implicated in the pathogenesis of severe SARS-CoV-2 infection. However, it remains to be determined whether increased complement is a broad indicator critical illness (and thus, no different COVID-19). It also unclear which pathways are contributing COVID-19, and if associated with certain features infection, such as endothelial injury hypercoagulability. To address these questions, we investigated plasma from patients COVID-19 prospectively enrolled at two...
Background. Mitochondrial DNA (MT-DNA) are intrinsically inflammatory nucleic acids released by damaged solid organs. Whether circulating cell-free MT-DNA quantitation could be used to predict the risk of poor COVID-19 outcomes remains undetermined.
Mycobacterium tuberculosis (M.tb.) replicates in host macrophages to cause tuberculosis. We have investigated the role of miRNAs M.tb.-infected murine RAW264.7 cells and bone marrow-derived (BMDMs), focusing on miR-155, most highly upregulated miRNA. observed that miR-155 upregulation is directly linked attenuation expression BTB CNC homology 1 (Bach1) SH2-containing inositol 5'-phosphatase (SHIP1). Bach1 a transcriptional repressor haem oxygenase-1 (HO-1), whereas SHIP1 inhibits activation...
For efficient clearance of Mycobacterium tuberculosis (Mtb), macrophages tilt towards M1 polarization leading to the activation transcription factors associated with production antibacterial effector molecules such as nitric oxide (NO) and proinflammatory cytokines interleukin 1 β (IL-1β) tumor necrosis factor α (TNF-α). At same time, resolution inflammation is M2 increased arginase IL-10. The transcriptional post-transcriptional mechanisms that govern balance between polarization,...
Autophagy plays a crucial role in the control of bacterial burden during Mycobacterium tuberculosis infection. MicroRNAs (miRNAs) are small non-coding RNAs that regulate immune signalling and inflammation response to challenge by pathogens. Appreciating potential host-directed therapies designed autophagy mycobacterial infection, we focused on miRNAs regulating M. tuberculosis-induced macrophages. Here, demonstrate infection leads downregulation miR-17 concomitant upregulation its targets...
The complement component C3 is a fundamental plasma protein for host defense, produced largely by the liver. However, recent work has demonstrated critical importance of tissue-specific expression in cell survival. Here, we analyzed effects local versus peripheral sources model acute bacterial pneumonia induced Pseudomonas aeruginosa . Whereas mice with global deficiency had severe pneumonia-induced lung injury, those deficient only liver-derived remained protected, comparable to wild-type...
ABSTRACT Complement activation has been implicated in the pathogenesis of severe SARS-CoV-2 infection. However, it remains to be determined whether increased complement is a broad indicator critical illness (and thus, no different COVID-19). It also unclear which pathways are contributing COVID-19, and, if associated with certain features infection, such as endothelial injury and hypercoagulability. To address these questions, we investigated plasma from patients COVID-19 prospectively...
Abstract The genome of M. tuberculosis (Mtb) encodes eleven paired two component systems (TCSs) consisting a sensor kinase (SK) and response regulator (RR). SKs sense environmental signals triggering RR-dependent gene expression pathways that enable the bacterium to adapt in host milieu. We demonstrate conserved motif present C-terminal domain regulates DNA binding functions OmpR family Mtb RRs. Molecular docking studies against this helped identify molecules with thiazolidine scaffold...
Paired two-component systems (TCSs), having a sensor kinase (SK) and cognate response regulator (RR), enable the human pathogen Mycobacterium tuberculosis to respond external environment persist within its host. Here, we inactivated SK gene of TCS MtrAB, mtrB, generating strain ΔmtrB. We show that mtrB loss reduces bacterium's ability survive in macrophages increases association with autophagosomes autolysosomes. Notably, ΔmtrB was markedly defective establishing lung infection mice, no...
Peptidyl prolyl isomerases (PPIases) are well‐conserved protein‐folding enzymes that moonlight as regulators of bacterial virulence. isomerase A, PPiA (Rv0009) is a secretory protein Mycobacterium tuberculosis possesses sequence and structural similarity to eukaryotic cyclophilins. In this study, we validated the interaction with stimulator interferon genes (STING) using both, Escherichia coli ‐based mammalian in vitro expression systems. pull‐down assays confirmed cytosolic domain STING...
The complement system is a family of proteins that facilitate immune resistance by attacking microbes to decrease pathogen burden. As result, deficiencies certain result in recurrent bacterial infections, and can also acute lung injury (ALI). We others have shown C3 present both nonimmune cells, modulates cellular functions such as metabolism, differentiation, cytokine production, survival. Although the emerging roles implications for host responses ALI, key questions remain vis-a-vis...
The solar systems are an intense need to full fill the energy requirement of developing countries like India.Where, thermal and photovoltaic two methods utilize directly from sun.In these equipments (e.g.flat plat collector Photovoltaic panel) kept in tilted position for absorbing maximum flux.Hence, finding optimum tilt angle is problem optimization.Therefore, this paper model driven optimization approach such as particle swarm (PSO) estimator has been proposed find its results compared...
Abstract Intracellular complement proteins such as C3 and FB play protective role during stress-induced cell death. C3−/−and Cfb−/−mice had worse Pseudomonas aeruginosa (P.a.)-induced acute lung injury at 24 h post infection compared to wildtype (WT) mice. However, the mechanism by which exerts these functions is unclear. We performed CRISPR-induced deletion of C3and CFBin BEAS-2B cells, a human tracheobronchial airway epithelial line assessed key findings in primary cells. also tested our...
Abstract The complement component C3 is a fundamental plasma protein for host defense. However, recent work has demonstrated the critical importance of local expression in cell survival. Here we analyzed effects versus peripheral sources model bacterial pneumonia. While mice with global deficiency had severe pneumonia-induced lung injury, those deficient liver-deficient remain protected, comparable to wildtype mice. Human transcriptome analysis showed secretory epithelial cells are major...
The present research proposes the novel corona virus kit using silicon based two (2D) photonic structure. basic principle of estimation different viruses relies on computation reflectance, absorbance, and transmittance at signal 412 nm. Here reflectance is investigated through analysis band gap where absorbance calculated analytical treatment. investigation made for such as N5H1, N5H2, H9N2, H4N6, FAdV, IBV. numerical indicates that sample could be affected by if transmitted lies with red...