A5101509211- Pneumonia and Respiratory Infections
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Respiratory viral infections research
- Plant Pathogens and Fungal Diseases
- Antibiotic Resistance in Bacteria
- Child Nutrition and Water Access
- HIV/AIDS Research and Interventions
- Intraocular Surgery and Lenses
- Sex work and related issues
- Viral gastroenteritis research and epidemiology
- HIV, Drug Use, Sexual Risk
- Bacterial Genetics and Biotechnology
- Retinal and Macular Surgery
- Sinusitis and nasal conditions
- Demographic Trends and Gender Preferences
- Adolescent Sexual and Reproductive Health
- interferon and immune responses
- Dental Anxiety and Anesthesia Techniques
- Gender, Labor, and Family Dynamics
- Birth, Development, and Health
- Child Nutrition and Feeding Issues
- Emotional Intelligence and Performance
- Ophthalmology and Visual Impairment Studies
- Autophagy in Disease and Therapy
Bose Institute
2014-2025
Punjab Technical University
2025
Indo Soviet Friendship College of Pharmacy
2025
Translational Health Science and Technology Institute
2024
Lutheran Hospital
2024
King George's Medical University
2018-2022
Creative Commons
2022
University College of Medical Sciences
2000-2021
Guru Teg Bahadur Hospital
2019-2021
Tribhuvan University
2005-2021
Patient-level data on life-threatening respiratory syncytial virus (RSV) infection in children low-income and lower-middle-income countries (LMICs) are scarce, this scarcity might limit demand for RSV interventions LMICs who rely support from Gavi, the Vaccine Alliance. We aimed to describe characteristics of RSV-positive younger than 2 years were admitted paediatric intensive care units (PICUs) with extended severe acute (eSARI) Gavi-eligible countries.
Coordinated bacterial cell septation and wall biosynthesis require formation of protein complexes at the sites division elongation, in a temporally controlled manner. The players these remain incompletely understood mycobacteria. Using vitro vivo assays, we showed that Rv2147c (or SepF) Mycobacterium tuberculosis interacts with principal driver cytokinesis, FtsZ. SepF also itself both vivo. Amino acid residues 189A, 190K 215F are required for FtsZ-SepF interaction, conserved across...
Cyclic-di-guanosine monophosphate (c-di-GMP) plays an important role in bacterial signalling networks. C-di-GMP exerts a regulatory function through binding to diverse molecules that include transcription factors, riboswitches and sensor kinases (SKs), thereby regulating processes. Here, we demonstrate the crosstalk between c-di-GMP SK MtrB of Mycobacterium tuberculosis . phosphorylates regulates its cognate response regulator MtrA. binds directly cytosolic domain inhibit...
CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) is a groundbreaking gene-editing technology that enables scientists to make precise changes the DNA of living organisms. It was first discovered in Escherichia coli and emerged as breakthrough tool molecular biology. This technique essential because its adaptability, affordability, ease use. uses adaptive immune response bacteria archaea repel viral invasions. significantly influences drug discovery, functional genomics,...
This comprehensive review critically examines the gut-brain axis (GBA) and its implications in autism spectrum disorder (ASD). The GBA is a complex, bidirectional communication network that integrates gastrointestinal tract, central nervous system, gut microbiota. mediated through various physiological pathways, including enteric system (ENS), vagus nerve, immune responses, metabolic activities of microorganisms. ASD, developmental marked by social impairments repetitive behaviors, presents...
Aim: The aim of this study is to observe changes in endothelial cell count post-Descemet's stripping keratoplasty (DSEK) and analyze the correlation between pre- postDSEK counts see if a higher donor improves graft longevity. Settings Design: A prospective cross-sectional observational was performed at Medical University from August 2020 July 2021. Materials Methods: This conducted among 19 DSEK patients. Preoperative postoperative density (ECD) measured using clinical specular microscopy...
Pneumonia in young children is still the most frequent cause of death developing countries. We aimed to identify predictors for recovery and treatment failure hospitalized with severe pneumonia.We enrolled 610 Nepalese children, aged 2 - 35 months from February 2006 June 2008. Study participants were provided standard pneumonia followed up until discharge. Three multiple regression models representing clinical variables, radiological combined all including C-reactive protein (CRP) viral...
Abstract The genome of M. tuberculosis (Mtb) encodes eleven paired two component systems (TCSs) consisting a sensor kinase (SK) and response regulator (RR). SKs sense environmental signals triggering RR-dependent gene expression pathways that enable the bacterium to adapt in host milieu. We demonstrate conserved motif present C-terminal domain regulates DNA binding functions OmpR family Mtb RRs. Molecular docking studies against this helped identify molecules with thiazolidine scaffold...
Mycobacterium tuberculosis ( Mtb ) is the causative agent of tuberculosis. Here, a macrophage infection model was used to unravel role histone deacetylase sirtuin 6 (SIRT6) in ‐triggered regulation innate immune response. downregulated microRNA‐26a and upregulated its target SIRT6. SIRT6 suppressed glycolysis expression HIF‐1α‐dependent glycolytic genes during infection. In addition, regulated levels intracellular succinate which controls stabilization HIF‐1α, as well release interleukin...
Peptidyl prolyl isomerases (PPIases) are well‐conserved protein‐folding enzymes that moonlight as regulators of bacterial virulence. isomerase A, PPiA (Rv0009) is a secretory protein Mycobacterium tuberculosis possesses sequence and structural similarity to eukaryotic cyclophilins. In this study, we validated the interaction with stimulator interferon genes (STING) using both, Escherichia coli ‐based mammalian in vitro expression systems. pull‐down assays confirmed cytosolic domain STING...
Autophagy is an intracellular catabolic process that required to maintain cellular homeostasis. Pathogen-elicited host cell autophagy may favour containment of infection or help in bacterial survival. Pathogens have developed the ability modulate autophagy. The secreted antigen HP0175, a peptidyl prolyl cis,trans isomerase Helicobacter pylori, has moonlighting functions with reference cells. Here we show it executes gastric epithelial dependent on unfolded protein response (UPR) activates...
The resuscitation-promoting factors of Mycobacterium tuberculosis are hydrolytic enzymes, which required for resuscitation dormant cells. RpfB, a peptidoglycan remodelling enzyme similar to the lytic transglycosylase Escherichia coli, is reactivation M. from chronic infection in vivo, underscoring need understand its transcriptional regulation. Here, we identified and translational start points rpfB, suggested rpf promoter-driven GFP expression vitro transcription assays that possibly occurs...
Paired two-component systems (TCSs), having a sensor kinase (SK) and cognate response regulator (RR), enable the human pathogen Mycobacterium tuberculosis to respond external environment persist within its host. Here, we inactivated SK gene of TCS MtrAB, mtrB, generating strain ΔmtrB. We show that mtrB loss reduces bacterium's ability survive in macrophages increases association with autophagosomes autolysosomes. Notably, ΔmtrB was markedly defective establishing lung infection mice, no...
Birth weight remains an important factor affecting infant and child mortality. Many factors influence the occurrence of low birth (LBW). The present study was conducted to prevalence determinants LBW in urban resettlement area Delhi. A baseline survey done enroll all pregnant women area. These were followed up every month till outcome. 39.1%. Occurrence related age, parity, height mother. In multiple logistic regression analysis, mother's parity alone emerged as significant variables. On...
1. Properties of paradichlorobenzene in causing fragmentation chromosomes have been worked out after trials different species Monocotyledons as well Dicotyledons. Six under Dicotyledons and ten were used for the purpose.2. Different require periods treatment chemical breakage their chromosomes. Dicotyledons, generally, longer than Monocotyledons.3. The results recovery experiments discussed potentiality mutagenic purposes has pointed out.4. In fixation schedules chromosome analysis, a...
Pneumonia is among the leading causes of illness and death in children <5 years age worldwide. There little information on viral etiology severe pneumonia low-income countries, where disease burden particularly high.We analyzed nasopharyngeal aspirates from 629 2 to 35 months meeting World Health Organization criteria for presenting at Kanti Children's Hospital, Kathmandu, Nepal, January 2006 through June 2008. We examined one specimen each child 7 respiratory viruses using reverse...
Partial nail avulsion with lateral chemical matricectomy is the treatment of choice for ingrown toenails. Phenol (88%) most widely used agent but prolonged postoperative drainage and collateral damage are common. Sodium hydroxide (NaOH) 10% has fewer side-effects.Adult, consenting patients toenails were alternately allocated into two groups in order their joining study, to receive either 88% phenol (Group 1, n = 26) or NaOH 0, 23) matricectomy. The as well statistician blinded being used....