The tyrosine kinases Flt4, Flt1, and Flk1 (or KDR) constitute a family of endothelial cell-specific receptors with seven immunoglobulin-like domains split kinase domain. Flt1 have been shown to play key roles in vascular development; these two bind are activated by growth factor (VEGF). No ligand has identified for whose expression becomes restricted during development the lymphatic endothelium. We cDNA clones from human glioma cell line that encode secreted protein 32% amino acid identity...

Abstract Background A wide range of organisms that enter the eye following ocular trauma can cause endophthalmitis. This study was to investigate spectrum pathogens and antibiotic susceptibility bacterial isolates from a large cohort post-traumatic endophthalmitis cases. Methods retrospective 912 patients treated at tertiary eye-care center in China performed. The associations between risk factors most common isolated were investigated by Chi square Test. percent susceptibilities for first...

AbstractLiver regeneration is a rapid and concerted response to injury, in which growth factor-generated intracellular signals result activation of transcription factors, DNA synthesis, hepatocyte proliferation. However, the link between cytoplasmic resulting proliferative liver injury remains be elucidated. We show here that association Gab1 adaptor protein Shp2 tyrosine phosphatase critical event at early phase regeneration. Partial hepatectomy (PH) rapidly transiently induced assembly...

Abstract Background The baseline data pertaining to the national epidemiological survey of infectious keratitis remain scarce in China, and currently there is no corneal blindness control strategy developed by nation. Methods Geographically defined cluster sampling was used randomly select a cross-section residents from representative urban rural populations Hubei Province. Participants were selected village registers, followed door-to-door household visits. assessment items included...

Rhegmatogenous retinal detachment associated with choroidal (RRDCD) is known for its rapid progression and poor prognosis, making it a subject of significant clinical interest due to complex pathogenesis. This study aims utilize mass spectrometry proteomic analysis vitreous humor identify proteins biomarkers critical the pathophysiology RRDCD. Data-independent acquisition (DIA) was employed analyze samples from RRDCD (RRD) patients. The focused on identifying differentially expressed (DEPs)...

Abstract Accumulating evidence has indicated that MEG3 can serve as a tumor suppressive lncRNA in various tumors. It is aberrantly expressed multiple cancers. However, the biological roles of melanoma are poorly understood. Therefore, our study, we concentrated on mechanism progression. First, observed was obviously decreased cells including A375, SK‐MEL‐1, B16, and A2058 compared to human epidermal melanocytes HEMa‐LP. restored by transfecting LV‐MEG3 A375 cells. Subsequently, found...

Abstract Background Spinal cord injury (SCI) is a disabling disorder, resulting in neurological impairments. This study investigated the mechanism of methyltransferase-like 14 (Mettl14) on apoptosis spinal neurons during SCI repair by mediating pri-microRNA (miR) dependent N6-methyladenosine (m6A) methylation. Methods The m6A content total RNA and Mettl14 levels tissues rats were detected. expression was intervened to examine motor function, neuron apoptosis, recovery neurites. cell model...

Background. Serine proteinase inhibitors, clade B, member 3 (SerpinB3) and B4 are highly similar in amino acid sequences associated with inflammation regulation. We investigated SerpinB3 expression their roles chronic rhinosinusitis nasal polyps (CRSwNP). Methods. The of mucosa tissues, brush cells, secretions from CRSwNP patients was measured, regulation by inflammatory cytokines were investigated. Their functions also analyzed using air–liquid interface (ALI)-cultured primary human...

AbstractSelenium compounds as effective chemopreventive agents can induce apoptosis in tumor cells, and reactive oxygen species (ROS) are important mediators induced by various stimuli including agents. The major mitogen-activated protein kinases (MAPKs), c-JUN N-terminal kinase (JNK), p38 extracelluar signal-regulated (ERK) three that have been shown to regulate apoptosis. In this study, we showed selenite-induced HepG2 cells was mediated ROS activated JNK selenite-treated a dose-...

Purpose Preservice teacher (PST) professional development programs are crucial for cultivating high-quality STEAM teachers of the future, significantly impacting quality regional education. The Guangdong-Hong Kong-Macao Greater Bay Area, as a region cross-border cooperation, integrates resources and advantages Guangdong, Hong Kong, Macao, possessing rich cultural heritage innovative capabilities. Transdisciplinary Education Cultural Inheritance (C-STEAM) is an effective approach to promoting...

Abstract Background Molecular signatures of chronic rhinosinusitis with nasal polyps (CRSwNP) related to macrophages remain unclear. This study aimed develop a macrophage‐associated diagnostic signature for CRSwNP. Methods Transcriptome data from 54 patients CRSwNP and 37 healthy controls across GSE136825, GSE36830, GSE72713 were used identify differentially expressed genes (DEGs) between two groups. Gene Set Enrichment Analysis Weighted Co‐Expression Network pinpointed crucial pathways gene...

Abstract Objectives Degenerative disc disease is characterized by an enhanced breakdown of its existing nucleus pulposus (NP) matrix due to the dysregulation enzymes and factors. Ubiquitin‐specific protease 15 (USP15) reported be abnormal in certain human diseases. However, role NP degeneration remains unclear. Therefore, we aimed explore function USP15 degenerative cell specimens. Methods We induced gene silencing overexpression cells using RNA interference (RNAi) a lentiviral vector,...

Bone mesenchymal stem cells are an attractive source of with potential applications in ocular regenerative medicine. However, the low differentiation efficiency traditional two-dimensional (2-D) culture system limits their application for clinical therapy. Here, we describe a simple and innovative 3-D environment assess bone to differentiate into variety cell types system.Bone rhesus monkey were isolated cultured using density gradient centrifugation adherence screening method. Cells at...

Exposure of mammalian cells to UV irradiation leads activation the c-Jun NH2-terminal protein kinase (JNK) pathway, which is associated with cell apoptosis. However, molecular mechanism for JNK by exposure not fully understood. We show here an essential role a multisubstrate adapter, Gab1, in this signaling cascade. Gab1-deficient mouse fibroblast were defective induction activity or heat shock, and defect was rescued reintroduction Gab1 into Gab1−/− cells. Consistently, displayed reduced...

Bone marrow mesenchymal stem cells can reduce retinal ganglion cell death and effectively prevent vision loss. Previously, we found that during differentiation, female rhesus monkey bone acquire a higher neurogenic potential compared with male cells. This suggests have stronger neuroprotective effect than Here, first isolated cultured from rats by density gradient centrifugation. Retinal tissue newborn was prepared enzymatic digestion to obtain primary Using the transwell system, were...