A5100756116- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Protein Kinase Regulation and GTPase Signaling
- Eicosanoids and Hypertension Pharmacology
- Drug Transport and Resistance Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Monoclonal and Polyclonal Antibodies Research
- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Protease and Inhibitor Mechanisms
- TGF-β signaling in diseases
- Flavonoids in Medical Research
- Hormonal Regulation and Hypertension
- Immunotherapy and Immune Responses
- Cancer Mechanisms and Therapy
- Chemokine receptors and signaling
- Nitric Oxide and Endothelin Effects
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
Shandong First Medical University
2025
Shaanxi University of Science and Technology
2024
The First Affiliated Hospital, Sun Yat-sen University
2017-2022
Sun Yat-sen University
2017-2022
Dalian Medical University
2009-2021
Shaanxi Provincial People's Hospital
2021
Shanxi Medical University
2021
Shandong Provincial QianFoShan Hospital
2020
Shandong University
2020
Huazhong University of Science and Technology
2018-2019
Abstract Macrophages play an important role in the pathogenesis of chronic inflammatory disease. Activation these phagocytes induces production proinflammatory cytokines, such as IL-1 and TNF-α generation reactive oxygen species (ROS), superoxide anion (O2•–). Recently, we found that treatment human monocytic cells (MonoMac1) isolated monocytes resulted up-regulation NADPH oxidase gene, neutrophil cytosolic factor 2 (NCF2). These results suggested TNF-α, produced by activated macrophages,...
Abstract Background The development of chemoresistance and metastasis are the leading causes death for gastric cancer (GC) patients, however, molecular mechanisms involved remain unclear. Dysregulation miRNAs is associated with a variety disease, including GC. Recently, microarray profiling analysis revealed that miR-939 was dysregulated in human GC samples, but role has not been intensively investigated. Methods In present study, we firstly examined expression pattern two independent...
Reactive oxygen species (ROS) signal vital physiological processes including cell growth, angiogenesis, contraction, and relaxation of vascular smooth muscle. Because cytochrome P-450 family 4 (CYP4)/20-hydroxyeicosatetraenoic acid (20-HETE) has been reported to enhance pulmonary tone, endothelial nitric oxide synthase function, we explored the potential this system stimulate bovine artery (BPAEC) ROS production. Our data are first demonstrate that 20-HETE increases in BPAECs a time-...
Abstract Accumulating evidence indicates that heat shock protein (HSP) 60 is strongly associated with the pathology of atherosclerosis (AS). However, precise mechanisms by which HSP60 promotes remain unclear. In present study, we found mRNA and expression levels in thoracic aorta are enhanced not only a mouse model AS but also high-fat diet (HFD) mice. secretion was activated platelet-derived growth factor-BB (PDGF-BB) interleukin (IL)-8 both human umbilical vein endothelial cells (HUVECs)...
To identify the interaction between known regulators of atherosclerosis, microRNA-92a (miR-92a), Rho-associated coiled-coil-forming kinase (ROCK) and myosin light chain (MLCK), we examined their expressions during proliferation migration platelet-derived growth factor-BB (PDGF-BB)-regulated vascular smooth muscle cells (VSMCs), both in vivo vitro. During formation atherosclerosis plaque mice, a parallel increase expression levels MLCK miR-92a was observed while reduced ML-7 (an inhibitor...
20-Hydroxyeicosatetraenoic acid (20-HETE) is an endogenous cytochrome P-450 product present in vascular smooth muscle and uniquely located the endothelium of pulmonary arteries (PAs). 20-HETE enhances reactive oxygen species (ROS) production bovine PA endothelial cells (BPAECs) NADPH oxidase-dependent manner postulated to promote angiogenesis via activation this pathway systemic beds. We tested capacity or a stable analog compound, 20-hydroxy-eicosa-5(Z),14(Z)-dienoic acid, enhance survival...
Abstract Accumulating evidence has indicated that MEG3 can serve as a tumor suppressive lncRNA in various tumors. It is aberrantly expressed multiple cancers. However, the biological roles of melanoma are poorly understood. Therefore, our study, we concentrated on mechanism progression. First, observed was obviously decreased cells including A375, SK‐MEL‐1, B16, and A2058 compared to human epidermal melanocytes HEMa‐LP. restored by transfecting LV‐MEG3 A375 cells. Subsequently, found...
Abstract It has been known that Rho‐associated protein kinase (ROCK) signaling regulates the migration of vascular smooth muscle cells (VSMCs). However, isoform‐specific roles ROCK and its underlying mechanism in VSMC are not well understood. The current study thus aimed to investigate ROCK1/2 their relationship MAPK pathway platelet‐derived growth factor (PDGF)‐induced rat aorta by manipulating gene expression. results revealed ROCK1 small interfering ribonucleic acid (siRNA) rather than...
We have shown that 20-hydroxyeicosatetraenoic acid (20-HETE) increases both superoxide and nitric oxide (NO) production in bovine pulmonary artery endothelial cells (BPAECs). The current study was designed to determine mechanisms underlying 20-HETE-stimulated NO release, particularly the role of NADPH oxidase, reactive oxygen species, PI3-kinase stimulated release. Intracellular hydrogen peroxide (H(2)O(2)) were detected by dichlorofluorescein or dihydrorhodamine diaminofluorescein...
Abstract A 1933 bp cDNA fragment, coding a truncated testis‐specific novel nucleoporin, was isolated from human testis λZAPII library, designated as BS‐63 and assigned GenBank accession number: U64675. By applying the methods of rapid amplification ends (5′ RACE) PCR, full‐length composed 5475 obtained. contained an open reading frame consisting 1765 codons XFXFG or GLFG repetitive sequence motifs. These motifs are structural characteristic nucleoporins. has high homology with Nup358/Ran...
Atherosclerosis is a chronic inflammatory disease with multiple contributing factors. Hyperlipidemia one of the major independent risks, and interleukin-8 (IL-8), as an factor, plays important role in development atherosclerosis. The aims study were to examine therapeutic efficacy G31P, antagonist IL-8 receptor, mouse model hyperlipidemia potential mechanisms G31P through vascular smooth muscle cell (VSMC) proliferation migration line. In vivo study: Male BALB/c mice fed high-fat diet for 6...
Abstract Plasma C‐reactive protein ( CRP ) concentration is associated positively with cardiovascular risk, including dyslipidemia. We suggested a regulating role of on pro‐protein convertase subtilisin/kexin type 9 PCSK 9), key regulator low‐density lipoprotein LDL metabolism, and demonstrated the as pathway linking regulation. Firstly, experiments were carried out in presence human mRNA expression receptor LDLR hepatoma cell line HepG2 cells. Treatment (10 μg/ml) enhanced significantly...
Transmembrane protein 98 (TMEM98), known as a novel gene related to lung cancer, hepatocellular carcinoma, differentiation of T helper 1 cells and normal eye development, has no defined role reported in terms atherosclerosis (AS). To investigate the potential involvement TMEM98 during AS processes, its obvious secretion expression been initially characterized hyperlipidemia patients' serum mice's respectively. We then explored possible pathogenesis vitro. IL-8, pro-atherogenesis cytokine,...
Background: Golgi phosphoprotein 3 (GOLPH3) overexpression was recently reported to be associated with a poor clinical outcome in patients colorectal cancer (CRC). However, the underlying molecular mechanism through which GOLPH3 promotes CRC metastasis remains poorly understood. Methods: In vitro genetic ablation of performed using siRNA transfection, and stably overexpressed colon cell line constructed lentivirus system. Cell invasion migration assays were conducted or without Matrigel....
Context: Atherosclerosis is a chronic inflammatory disease in which the plaques were built up inside of artery. Interleukin-8 (IL-8, CXCL8) an factor, known to play important role development atherosclerosis. G31P antagonist IL-8 receptor, plays roles vascular smooth muscle cell (VSMC) proliferation and migration. Objective: This study investigate therapeutic effect on atherosclerosis through mouse model.Materials methods: A model was generated feeding ApoE–/– mice with high fat diet for 12...