A5032703866- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Plant Pathogens and Fungal Diseases
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Polymer Surface Interaction Studies
- Fungal Biology and Applications
- Poxvirus research and outbreaks
- Proteoglycans and glycosaminoglycans research
- Wound Healing and Treatments
- interferon and immune responses
- Toxin Mechanisms and Immunotoxins
- Animal Virus Infections Studies
- RNA regulation and disease
- Forest, Soil, and Plant Ecology in China
- T-cell and B-cell Immunology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Environmental and Agricultural Sciences
- Plant Ecology and Soil Science
- Mycotoxins in Agriculture and Food
- RNA modifications and cancer
- Cancer Cells and Metastasis
Central South University
2023-2024
Institute of Tibetan Plateau Research
2018-2024
Chinese Academy of Sciences
2013-2024
Central South University of Forestry and Technology
2023-2024
University of Chinese Academy of Sciences
2018-2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2023-2024
Sun Yat-sen University
2023-2024
Xinjiang Institute of Ecology and Geography
2024
Northwest Institute of Plateau Biology
2024
Zhejiang Chinese Medical University
2020-2024
Cancer-associated fibroblasts (CAFs) play a key role in the cancer process, but research progress is hampered by paucity of preclinical models that are essential for mechanistic dissection cell-CAF interactions. Here, we aimed to establish 3-dimensional (3D) organotypic co-cultures primary liver tumor-derived organoids with CAFs, and understand their interactions response treatment.Liver tumor CAFs were cultured from murine human tumors. 3D co-culture Transwell culture systems established...
In cell cultures, HSV-1 replication is initiated by recruitment virion protein 16 of transcriptional factors and histone-modifying enzymes to immediate early (α) gene promoters. HSV establishes latent infections characterized suppression viral expression except for latency-associated transcripts (LATs) miRNAs. The virus reactivates in stressed neurons. A fundamental question how reactivation initiates the absence 16. We report following findings ganglion explant model. ( i ) Anti-nerve...
CD4+ type 1 T regulatory (Tr1) cells have a crucial role in inducing tolerance. Immune regulation by these is mainly mediated through the secretion of high amounts IL-10. Several studies suggested that this population may be involved tumor-mediated immune-suppression. However, direct evidence for Tr1 human solid tumors lacking. Using ex vivo isolated from individuals with hepatocellular carcinoma (HCC; n = 39) or liver metastases colorectal cancer (LM-CRC; 60) we identify...
Metabolic reprogramming universally occurs in cancer. Mitochondria act as the hubs of bioenergetics and metabolism. The morphodynamics mitochondria, comprised fusion fission processes, are closely associated with mitochondrial functions often dysregulated In this study, we aim to investigate its functional consequences human liver We observed excessive activation tumor tissues from hepatocellular carcinoma (HCC) patients vitro cultured organoids cholangiocarcinoma (CCA). knockdown regulator...
Background & AimsTIGIT is a co-inhibitory receptor, and its suitability as target for cancer immunotherapy in HCC unknown. PD1 blockade clinically effective about 20% of advanced patients. Here we aim to determine whether co-blockade TIGIT/PD1 has added value restore functionality tumor-infiltrating T cells (TILs).MethodsMononuclear leukocytes were isolated from tumors, paired tumor-free liver tissues (TFL) peripheral blood patients, used flow cytometric phenotyping functional assays....
Abstract Background Immunotherapy with immune checkpoint inhibitors (ICIs) is being explored to improve cholangiocarcinoma (CCA) therapy. However, it remains difficult predict which ICI will be effective for individual patients. Therefore, the aim of this study develop a co-culture method patient-derived CCA organoids and cells, could represent anti-cancer immunity in vitro. Methods were co-cultured peripheral blood mononuclear cells or T cells. Flow cytometry, time-lapse confocal imaging...
ABSTRACT We have made two stocks of a herpes simplex virus 1 mutant lacking intact U S 5 and 6 open reading frames encoding glycoproteins J (gJ) D (gD), respectively. The stock designated gD −/+ , in cells carrying expressing gD, was capable productively infecting cells, whereas the −/− viral DNA sequences, known to attach but not initiate infection. report following. (i) Both induced apoptosis SK-N-SH cells. Thus, annexin V binding cell surfaces detected as early 8 h after (ii) or cloned...
Herpes simplex viruses replicate at the portal of entry into body and are transported retrograde to sensory neurons in which they can establish a silent, latent infection characterized by expression noncoding latency-associated transcript set microRNAs. At cell culture viral protein, VP16, recruits cellular proteins that initiate sequential derepression several kinetic classes genes. Earlier studies have shown upon reactivation virus ganglionic organ cultures all genes derepressed once, thus...
We report on the properties and function of two herpes simplex virus-1 (HSV-1) microRNAs (miRNAs) designated "miR-H28" "miR-H29." Both miRNAs accumulate late in productive infection at a time when, for most part, viral DNA proteins have been made. Ectopic expression miRNA mimics human cells before reduced accumulation mRNAs proteins, plaque sizes, vey low multiplicities yields. The specificity was tested ways. First, ectopic carrying mutations seed sequence ineffective. Second, similar tests...
Purpose: Liver metastasis develops in >50% of patients with colorectal cancer (CRC), and is a leading cause CRC-related mortality. We aimed to identify which inhibitory immune checkpoint pathways can be targeted enhance functionality intra-tumoral T-cells mismatch repair-proficient liver metastases (LM-CRC). Methodology: Intra-tumoral expression multiple molecules was compared among LM-CRC, peritoneal (PM-CRC) primary CRC. Expression also analyzed on leukocytes isolated from paired resected...
No curative treatment options are available for advanced hepatocellular carcinoma (HCC). Anti-PD1 antibody therapy can induce tumor regression in 20% of HCC patients, demonstrating that co-inhibitory immune checkpoint blockade has therapeutic potential this type cancer. However, whether agonistic targeting co-stimulatory receptors might be able to stimulate anti-tumor immunity is as yet unknown. We investigated the receptor GITR could reinvigorate ex vivo functional responses...
Significance The mechanism by which HSV enters into a silent, latent state in neurons remains major problem virology. only manifestations of viral gene expression during latency are the accumulation noncoding transcript and set microRNAs (miRNAs). Available data support hypothesis that these play role maintenance predict different miRNAs reactivation. This report ( i ) supports arise from multiple transcriptional events on basis location differences regulation productively infected cells ii...
A key property of herpes simplex viruses (HSVs) is their ability to establish latent infection in sensory or autonomic ganglia and reactivate on physical, hormonal, emotional stress. In latently infected ganglia, HSV expresses a long noncoding RNA set microRNAs, but viral proteins are not expressed. The mechanism by which reactivates unknown. question is, what the reactivation absence tegument that enable gene expression productive infection? Elsewhere we have reported use ganglionic organ...
In liver cancer tumor-infiltrating regulatory T cells (Ti-Treg) are potent suppressors of tumor-specific T-cell responses and express high levels the Treg-associated molecules cytotoxic lymphocyte-associated antigen 4 (CTLA-4) glucocorticoid-induced tumor necrosis factor receptor (GITR). this study, we have evaluated capacity GITR-ligation, CTLA-4-blockade a combination both treatments to alleviate immunosuppression mediated by Ti-Treg. Using ex vivo isolated from individuals with...
Background and Objectives Patients with isolated colorectal‐cancer‐liver‐metastases (CRCLM) frequently undergo metastatectomy. Tumor‐infiltrating‐lymphocytes (TILs) have prognostic potential in the setting of primary colorectal cancer, however, their role CRCLM is less studied. We aimed to study spatial distribution tumor‐infiltrating CD8 + cytotoxic T‐cells FoxP3 regulatory at metastatic site patients. Methods TILs were from fresh tissues 47 patients CRCLM. Archived paraffin‐embedded...
Abstract Tumor expression of immune co-inhibitory ligands, such as PD-L1 and Galectin-9, have potential prognostic value in Hepatocellular Carcinoma (HCC). Circulating levels these molecules, however, hardly been studied. This study aims to assess the significance circulating Galectin-9 patients with resected HCC, compare their intra-tumoral same molecules. Archived tissues stored peripheral blood samples from 81 who underwent HCC resection or liver transplantation, curative intent, were...
Abstract Background Patients with resected colorectal liver metastasis (CRLM) who display only the desmoplastic histopathological growth pattern (dHGP) exhibit superior survival compared to patients any non-desmoplastic (non-dHGP). The aim of this study was compare tumour microenvironment between dHGP and non-dHGP. Methods investigated in three cohorts chemo-naive surgically treated for CRLM. In cohort A semi-quantitative immunohistochemistry performed, B intratumoural peritumoural T cells...