A5023270457- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal and Macular Surgery
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Drug-Induced Ocular Toxicity
- Cell Adhesion Molecules Research
- 14-3-3 protein interactions
- Intraocular Surgery and Lenses
- Corneal surgery and disorders
- Alzheimer's disease research and treatments
- Corneal Surgery and Treatments
- Retinopathy of Prematurity Studies
- Ophthalmology and Visual Impairment Studies
- Methemoglobinemia and Tumor Lysis Syndrome
- Long-Term Effects of COVID-19
- RNA Interference and Gene Delivery
- Intramuscular injections and effects
- Computational Drug Discovery Methods
- Vascular Malformations Diagnosis and Treatment
- Systemic Sclerosis and Related Diseases
- Angiogenesis and VEGF in Cancer
- Bioinformatics and Genomic Networks
- Viral Infectious Diseases and Gene Expression in Insects
Massachusetts Eye and Ear Infirmary
2019-2025
Harvard University
2020-2025
Smith-Kettlewell Eye Research Institute
2020-2024
Hospital Universitario Puerta de Hierro Majadahonda
2021-2023
Instituto de Microcirugía Ocular
2022
Miranza
2022
Universidad de Valladolid
2014-2021
Hospital Clínico Universitario de Valladolid
2013-2015
Abstract We characterized the world’s second case with ascertained extreme resilience to autosomal dominant Alzheimer’s disease (ADAD). Side-by-side comparisons of this male and previously reported female ADAD homozygote for APOE3 Christchurch ( APOECh ) variant allowed us discern common features. The remained cognitively intact until 67 years age despite carrying a PSEN1 -E280A mutation. Like carrier, he had extremely elevated amyloid plaque burden limited entorhinal Tau tangle burden. He...
Abstract INTRODUCTION We discovered that the APOE3 Christchurch (APOE3Ch) variant may provide resistance to Alzheimer's disease (AD). This be due reduced pathological interactions between ApoE3Ch and heparan sulfate proteoglycans (HSPGs). METHODS developed characterized binding, structure, preclinical efficacy of novel antibodies targeting human ApoE‐HSPG interactions. RESULTS found one these antibodies, called 7C11, preferentially bound ApoE4, a major risk factor for sporadic AD, disrupts...
To describe a series of retinal acute toxicity cases with severe visual loss after intraocular use toxic perfluoro-octane (PFO). The clinical presentation is described, and the likely causes are analyzed. New biological methods for testing safety medical devices proposed.Information regarding eyes suffering events PFO was Four types spectroscopy, nuclear magnetic resonance, chromatography were used to identify potential contaminants. Cultures human pigment epithelial cells (ARPE-19) porcine...
Runt-related transcription factor 1 (RUNX1) acts as a mediator of aberrant retinal angiogenesis and has been implicated in the progression proliferative diabetic retinopathy (PDR). Patients with PDR, prematurity (ROP), wet age-related macular degeneration (wet AMD) have found to elevated levels Tumor Necrosis Factor-alpha (TNF-α) eye. In fibrovascular membranes (FVMs) taken from patients PDR RUNX1 expression was increased vasculature, while human microvascular endothelial cells (HRMECs),...
Abstract Proliferative vitreoretinopathy (PVR) is the leading cause of retinal detachment surgery failure. Despite significant advances in vitreoretinal surgery, it still remains without an effective prophylactic or therapeutic medical treatment. After ocular injury detachment, misplaced cells undergo epithelial to mesenchymal transition (EMT) form contractile membranes within eye. We identified Runt-related transcription factor 1 (RUNX1) as a gene highly expressed surgically-removed human...
Abstract Photoreceptor cell death and immune infiltration are two major events that contribute to retinal degeneration. However, the relationship between these has not been well delineated, primarily because of an inadequate understanding immunological processes involved in photoreceptor degeneration, especially peripheral leukocytes infiltrate subretinal space tissues. In this work, we characterized role leukocyte within detached retina. We observed CD45 + CD11b Ly6G neutrophils − Ly6C...
Messenger RNA (mRNA)–based therapies are a promising approach to medical treatment. Except for infectious diseases, no other disease has mRNA-based available. The eye is an ideal model mRNA therapeutic development because it requires limited dosing. Proliferative vitreoretinopathy (PVR) blinding condition caused by retinal detachment that now lacks available treatment, with surgery as the only treatment option. We previously implicated runt-related transcription factor-1 (RUNX1) driver of...
Abstract To investigate local cell death differences in the attached and detached retina at different regions a murine experimental retinal detachment model. Subretinal injection of sodium hyaluronate was performed eight-week-old C57BL/6J mice. Retinal interest were defined relation to their distance from peak detachment, as follows: (1) central; (2) paracentral; (3) apex; (4) base. At day 0, outer nuclear layer count for central, paracentral, apex, base 1247.60 ± 64.62, 1157.80 163.33,...
The aim of this study was to analyze the atrophic, tractional, and neovascular (ATN) components grading in highly myopic patients with dome-shaped macula (DSM) ridge-shaped (RSM).This a cross-sectional, noninterventional study. 57 eyes 38 different were included. They classified as DSM or RSM based on number radial scans that showed an inward protrusion ≥50 µm swept-source optical coherence tomography (SS-OCT) (12 = DSM; <12 RSM). All underwent complete ophthalmological examination...
To analyze predictors and develop predictive models of anatomic outcome in neovascular age-related macular degeneration (AMD) treated with as-needed ranibizumab after 4 years follow-up. A multicenter consecutive case series non-interventional study was performed. Clinical, funduscopic OCT characteristics 194 treatment-naïve patients AMD for at least 2 up to were analyzed baseline, 3 months each year until the end Baseline demographic angiographic also evaluated. R Statistical Software used...
To test the efficacy of runt-related transcription factor 1 (RUNX1) inhibition with topical nanoemulsion containing Ro5-3335 (eNano-Ro5) in experimental ocular neovascularization.Preclinical study.In vitro primary culture human retinal endothelial cell (HREC) culture. C57BL/6J 6- to 10-week-old male and female mice.We evaluated effect eNano-Ro5 proliferation, toxicity, migration HRECs. We used an alkali burn model corneal neovascularization a laser-induced choroidal vivo pathologic...
To study clinical features in patients with ridge-shaped macula (RSM) compared those dome-shaped (DSM) having been previously classified by the number of swept-source optical coherence tomography (SS-OCT) radial scans affected.Retrospective observational including 49 highly myopic eyes from 31 who underwent SS-OCT. DSM were defined as that showed a complete round inward convexity all their axes, presenting an ≥50 µm 12-line OCT scans. Eyes did not meet this criterion and had at least one...
Purpose: The objective of this study is to determine the expression and localization lymphotoxin alpha (LTA) in human retinas functionality one its polymorphisms rs2229094 (C13R) (T>C), previously associated with proliferative vitreoretinopathy (PVR) development. Materials methods: Total RNA from three healthy were extracted subjected reverse transcription-polymerase chain reaction (RT-PCR) analysis, using flanking primers LTA cDNA. In addition, eyes retinal detachment (RD) control...
Abstract Photoreceptor transplant has been put forward as a repair strategy to tackle degenerated retinas. Nonetheless, cell death and immune rejection seriously limit the success of this strategy, with only small fraction transplanted cells surviving. Improving survival is critical importance. Recent evidence identified receptor‐interacting protein kinase 3 (RIPK3) molecular trigger controlling necroptotic inflammation. However, its role in photoreceptor transplantation regenerative...
Abstract Background Prevalence of high myopia is continuously increasing, thus, patients affected with staphyloma are abundant worldwide. Assessment the quality vision in these mandatory for a proper clinical counselling, specially when undergoing surgical procedures that require intraocular lenses implantation. Thus, purpose study was to assess monochromatic higher order aberrations (HOAs) highly myopic eyes or without dome-shaped macula. Methods Participants underwent spectral-domain...
Purpose To compare the distribution of BAX G(-248)A and BCL-2 C(-938)A genotypes among European subjects undergoing rhegmatogenous retinal detachment surgery in relation to further development proliferative vitreoretinopathy (PVR). Methods A case-control gene association study as part Retina 4 Project was designed. Two promoter single nucleotide polymorphisms (rs2279115 rs4645878) were analysed by TaqMan 5'exonuclease allelic discrimination assay, using a StepOne® system 134 samples from...