A5086891601- Immune Cell Function and Interaction
- Biofuel production and bioconversion
- Immunotherapy and Immune Responses
- Corrosion Behavior and Inhibition
- Enzyme Production and Characterization
- Geomagnetism and Paleomagnetism Studies
- Monoclonal and Polyclonal Antibodies Research
- Bioactive Compounds and Antitumor Agents
- Immune Response and Inflammation
- Image and Video Quality Assessment
- Cancer Research and Treatments
- Hepatitis B Virus Studies
- Polysaccharides and Plant Cell Walls
- Aluminum Alloy Microstructure Properties
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Geology and Paleoclimatology Research
- Nanoparticle-Based Drug Delivery
- Hydrogen embrittlement and corrosion behaviors in metals
- Mesenchymal stem cell research
- Smart Materials for Construction
- Glycosylation and Glycoproteins Research
- Synthesis and Characterization of Heterocyclic Compounds
- GABA and Rice Research
- Circular RNAs in diseases
Brown Foundation
2020-2023
The University of Texas Health Science Center at Houston
2020-2023
China Agricultural University
2017-2022
Xi'an Jiaotong University
2018-2022
Kingmed Diagnostics
2018-2019
Chinese People's Liberation Army
2017-2019
82th Hospital of Pla
2003-2018
Beijing Institute of Geology for Mineral Resources
2017
Southeast University
2015
South China University of Technology
2005-2012
Background Immune exclusion (IE) where tumors deter the infiltration of immune cells into tumor microenvironment has emerged as a key mechanism underlying immunotherapy resistance. We recently reported novel role discoidin domain-containing receptor 1 (DDR1) in promoting IE breast cancer and validated its critical using neutralizing rabbit monoclonal antibodies (mAbs) multiple mouse models. Methods To develop DDR1-targeting mAb potential therapeutic, we humanized mAb9 with...
The xylanases with high specific activity and resistance to harsh conditions are of practical value for biomass utilization. In the present study, two new GH11 xylanase genes, MYCTH_56237 MYCTH_49824, have been cloned from thermophilic fungus Myceliophthora thermophila expressed in Pichia pastoris. activities purified reach approximately 1,533.7 1,412.5U/mg, respectively. Based on multiple template-based homology modeling, structures their catalytic domains predicted. Enzyme was more...
High metastatic rate and recurrence of tumor because circulating cells are seriously hinders for clinical therapy.Herein, we develop a novel, active-targeting nanotherapeutic by simultaneously loading doxorubicin (DOX) transferrin (Tf) onto bacterial magnetosomes (Tf-BMs-DOX) investigate its antitumor efficacy in vitro vivo.Drug release profiles indicated that Tf-BMs/BMs loaded with DOX were capable sustained drug release, suggesting reduce drugs required frequency administration enhance...
Objective: To explore the effect and mechanism of artesunate on γδ T cell-mediated antitumor immune responses against hepatoma carcinoma cells (HepG2) in vitro.Methods: Human or HepG2 were respectively treated with artesunate, subjected to co-culture as appropriate, following assays subsequently conducted: CCK8 examine cell viability; LDH release assay detect killing cells; flow cytometry expression perforin (PFP) granzyme B (GraB) ELISA evaluate levels TGF-β1 IL-10 collected supernatant...
Here, we report highly efficient degradation of xylan into xylose by a single multifunctional xylanolytic enzyme from the filamentous fungus Thermothelomyces thermophila (termed Ttxy43). Ttxy43 shows three different activities toward carbohydrates, β-xylosidase (80.8 U/mg), endoxylanase (105.42 and α-l-arabinofuranosidase (15.81 U/mg). Analysis catalytic mode action for birchwood-xylan (BWX) reveals that initially degrades to unbranched xylooligosaccharides (XOSs) (xylobiose, xylotriose,...
The lignocellulosic enzymes of Trichoderma asperellum have been intensely investigated toward efficient conversion biomass into high-value chemicals/industrial products. However, lack genome data is a remarkable hurdle for hydrolase systems studies. secretory newly isolated T. ND-1 during lignocellulose degradation are currently poorly known. Herein, high-quality genomic sequence ND-1, obtained by both Illumina HiSeq 2000 sequencing platforms and PacBio single-molecule real-time, has an...
α-L-arabinofuranosidase (ARA) with enhanced specific activity and in large amounts, is needed for a variety of industrial applications. To improve ARA production engineered methylotrophic yeast Pichia pastoris, genetically modified ara gene from Aspergillus niger ND-1 was investigated. Through codon optimization rational replacement α-factor signal peptide the native propeptide (MFSRRNLVALGLAATVSA), improved 2.61 ± 0.13 U/mL to 14.37 0.22 shaking flask culture (a 5.5-fold increase). Results...
Xylanases with high specific activity has been focused great interest as a useful enzyme in biomass utilization. The production of recombinant GH11 xylanase (MYCTH_56237) from Myceliophthora thermophila improved through N-terminal signal peptide engineering P. pastoris . newly (termed Mtxyn11C) was 442.53 to 490.7 U/mL, replacement α-factor the native segment (MVSVKAVLLLGAAGTTLA) Scaling up Mtxyn11C 7.5 L fermentor maximal rate 2503 U/mL. In this study, degradation efficiency further...
Aim: This study aimed to develop anthracycline-loaded bacterial magnetosomes (BMs) with enhanced anticancer efficiency and elucidate their endocytosis mechanism. Methods: Drug-loaded BMs (DBMs) were successfully prepared characterized. DBMs was investigated within HepG2 cells. The effect of studied both in vitro vivo. Results: Doxorubicin–BMs daunorubicin–BMs showed growth inhibitory vivo no notable toxicity normal tissues. internalized into cells through caveolae-mediated macropinocytosis....
The effect of pituitary adenylate cyclase activating polypeptide (PACAP) during traumatic brain injury (TBI) and whether it can modulate secondary has not been reported previously. present study evaluated the potential protective effects ventricular infusion PACAP in a rat model TBI.Male Sprague Dawley rats were randomly divided into 3 treatment groups (n=6, each): sham-operated, vehicle (normal saline)+TBI, PACAP+TBI. Normal saline or (1 μg/5 μL) was administered intracerebroventricularly...
HTTP adaptive streaming (HAS) technology has been increasingly employed by video service providers (VSPs) due to its prominent benefits such as reducing interruptions of playback and achieving higher bandwidth utilization outstanding quality experience (QoE). And many VSPs have deployed HAS applications in the media cloud provide large-scale services. At present, research into typically focuses on management optimization resources, placement migration virtual machines data centers. However,...