A5014177345- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Salmonella and Campylobacter epidemiology
- RNA modifications and cancer
- Plant and Fungal Interactions Research
- Circular RNAs in diseases
- Clostridium difficile and Clostridium perfringens research
- Molecular Biology Techniques and Applications
- Animal Virus Infections Studies
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- GDF15 and Related Biomarkers
- Histone Deacetylase Inhibitors Research
- Genomics and Phylogenetic Studies
- Axon Guidance and Neuronal Signaling
- Protein Kinase Regulation and GTPase Signaling
Duke Kunshan University
2020-2024
Nanjing Forestry University
2023
City University of Hong Kong
2017-2022
Guangzhou University of Chinese Medicine
2020-2022
Harvard University
2000-2020
City University of Hong Kong, Shenzhen Research Institute
2017-2020
Boston Children's Hospital
2013-2020
First Affiliated Hospital of Guangzhou University of Chinese Medicine
2019
Jiangxi Maternal and Child Health Hospital
2019
Nanchang University
2014-2017
Alternative splicing of precursor mRNA is an essential mechanism to increase the complexity gene expression, and it plays important role in cellular differentiation organism development. Regulation alternative a complicated process which numerous interacting components are at work, including cis-acting elements trans-acting factors, further guided by functional coupling between transcription splicing. Additional molecular features, such as chromatin structure, RNA structure initiation or...
ABSTRACT Tumour cells release large quantities of extracellular vesicles (EVs) to mediate their interactions with other in the tumour microenvironment. To identify host that naturally take up EVs from cells, we created breast cancer cell lines secreting fluorescent EVs. These are taken most robustly by fibroblasts within RNA sequencing indicated miR‐125b is one abundant microRNAs secreted mouse triple‐negative 4T1 and 4TO7 cells. Treatment leads an increase fibroblast activation isogenic...
Summary Salicylic acid (SA) plays important roles in plants, most notably the induction of systemic acquired resistance (SAR) against pathogens. A non‐destructive situ assay for SA would provide new insights into functions SAR and other SA‐regulated phenomena. We assessed a genetically engineered strain Acinetobacter sp. ADP1, which proportionally produces bioluminescence response to salicylates including methylsalicylate, as reporter salicylate accumulation apoplast plant leaves. was...
Abstract Endosomal escape and subsequent cytosolic delivery of small interfering RNA (siRNA) therapeutics is believed to be highly inefficient. Since it has not been possible quantify amounts delivered siRNA at therapeutic doses, determining bottlenecks total efficiency difficult. Here, we present a confocal microscopy-based method fluorescently labeled during lipid-mediated delivery. This enables detection quantification sub-nanomolar release from individual events with measures...
Acinetobacter sp. ADP1 is a common soil-associated bacterium with high natural competency, allowing it to efficiently integrate foreign DNA fragments into its chromosome. This property was exploited engineer salicylate-inducible luxCDABE and green fluorescent protein (GFP) variants of ADP1. Specifically, ADPWH_lux displayed the higher sensitivity when comparing two (minimum detection c. 0.5-1 microM salicylate) faster turnover lux marker gene, making suitable for whole-cell luminescence...
Background Immunoglobulin A nephropathy (Ig AN ) is the most common type of primary glomerulonephritis in world. Reliable biomarkers are required for non‐invasive diagnosis and monitoring Ig . This study aims to investigate difference urinary exosomal micro RNA (mi expression profiles between patients with IgA healthy controls, which may provide clues identify novel potential mi renal diseases. Methods Urine samples were collected from eighteen controls Differential centrifugation was...
Extracellular vesicles (EVs) such as microvesicles (MIVs) play an important role in intercellular communications. MIVs are small membrane sized 100-1000 nm diameter that released by many types of cells, mesenchymal stem cells (MSCs), tumor and adipose-derived (ADSC). As EVs can carry out autocrine paracrine functions controlling multiple cell processes, it is conceivable be used delivery vehicles for treating several clinical conditions, to improve cardiac angiogenesis after myocardial...
Fishing miRNAs from individual living cells enables quasi–single-cell analysis for dissecting cellular heterogeneity.
Transcription factors (TFs) regulate transcription by binding to the specific sequences at promoter region. However, mechanisms and functions of TFs within coding (CDS) remain largely elusive in prokaryotes. To this end, we collected 409 data sets for bacterial TFs, including 104 chromatin immunoprecipitation sequencing (ChIP-seq) assays 305 from systematic evolution ligands exponential enrichment (SELEX) seven model bacteria. Interestingly, these displayed same capabilities both intergenic...
Osteoporosis, which typically affects postmenopausal women, is an osteolytic disease due to over-activation of osteoclasts. However, current drugs targeting osteoclast inhibition face various side effects, making natural compounds with great interest as alternative treatment options. Cycloastragenol (CAG) a triterpenoid multiple biological activities. Previously, CAG's activity against aging-related osteoporosis was reported, but the mechanisms actions for activities were not understood....
The use of combinatorial siRNAs shows great promise for drug discovery, but the identification safe and effective siRNA combinations remains challenging. Here, we develop a massively multiplexed technology systematic screening siRNA-based cocktail therapeutics. We employ composite micro-carriers that are responsive to near infrared light magnetic field achieve photoporation-facilitated transfection individual cells. Thus, randomized gene silencing by different formulations can be performed...
Abstract DNA damage repair through the nucleotide excision (NER) pathway is one of major reasons for decreased antitumor efficacy platinum‐based anticancer drugs that have been widely applied in clinic. Inhibiting intrinsic NER function may enhance activity cisplatin and conquer resistance. Herein, we report design, optimization, application a self‐assembled lipid nanoparticle (LNP) system to simultaneously deliver prodrug together with siRNA targeting endonuclease xeroderma pigmentosum...
Induced pluripotent stem cells (iPSCs) bear great potential for disease modeling, drug discovery, and regenerative medicine; however, the wide adoption of iPSC clinically relevant applications has been hindered by extremely low reprogramming efficiency. Here, we describe a high-efficiency cellular strategy puncturing with an array diamond nanoneedles, which is applied to temporally disrupt cell membrane in reversible minimally invasive format. This method enables cytoplasmic delivery...