A5047190410- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Lipid metabolism and biosynthesis
- SARS-CoV-2 detection and testing
- Long-Term Effects of COVID-19
- Diabetes and associated disorders
- Kawasaki Disease and Coronary Complications
- Animal Virus Infections Studies
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Macrophage Migration Inhibitory Factor
- Immune Response and Inflammation
- Occupational Health and Performance
- Enzyme Production and Characterization
- Global Health and Epidemiology
- Antifungal resistance and susceptibility
- Vaccine Coverage and Hesitancy
- Fungal Infections and Studies
- Endoplasmic Reticulum Stress and Disease
- Dermatological and COVID-19 studies
- Protein purification and stability
- Healthcare Systems and Reforms
- Eosinophilic Disorders and Syndromes
Rutgers, The State University of New Jersey
2021-2025
Rutgers Health
2023-2025
Rutgers New Jersey Medical School
2021-2024
Rutgers Sexual and Reproductive Health and Rights
2023-2024
University of Illinois Urbana-Champaign
2023
Alabama Agricultural and Mechanical University
2018-2020
University of Louisiana at Lafayette
2017
Abstract Dysregulated innate immune responses contribute to multisystem inflammatory syndrome in children (MIS-C), characterized by gastrointestinal, mucocutaneous, and/or cardiovascular injury occurring weeks after severe acute respiratory coronavirus 2 (SARS-CoV-2) exposure. To investigate functions, we stimulated ex vivo peripheral blood cells from MIS-C patients with agonists of Toll-like receptors (TLR), key response initiators. We found severely dampened cytokine and elevated gene...
Foam cells are dysfunctional, lipid-laden macrophages associated with chronic inflammation of diverse origin. The long-standing paradigm that foam cholesterol-laden derives from atherosclerosis research. We previously showed that, in tuberculosis, surprisingly accumulate triglycerides. Here, we utilized bacterial (Mycobacterium tuberculosis), fungal (Cryptococcus neoformans), and human papillary renal cell carcinoma (pRCC) models to address the need for a new explanation biogenesis. applied...
Abstract Background We studied risk factors, antibodies, and symptoms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in a diverse, ambulatory population. Methods A prospective cohort (n = 831) previously undiagnosed with SARS-CoV-2 underwent serial testing (SARS-CoV-2 polymerase chain reaction, immunoglobulin G [IgG]) for 6 months. Results Ninety-three participants (11.2%) tested SARS-CoV-2-positive: 14 (15.1%) asymptomatic, 24 (25.8%) severely symptomatic....
Much is to be learned about the interface between immune responses SARS-CoV-2 infection and vaccination. We monitored specific Spike Receptor-Binding-Domain (RBD) in convalescent individuals for eight months after diagnosis following Over time, neutralizing antibody responses, which are predominantly RBD specific, generally decreased, while RBD-specific memory B cells persisted. cell vaccination were more vigorous than those elicited by same subjects or infection-naïve comparators. Notably,...
Dysregulated innate immune responses contribute to multisystem inflammatory syndrome in children (MIS-C), characterized by gastrointestinal, mucocutaneous, and/or cardiovascular injury occurring weeks after SARS-CoV-2 exposure. To investigate functions MIS-C, we stimulated
Foam cells are dysfunctional, lipid-laden macrophages associated with chronic inflammation of diverse origin. The long-standing paradigm that foam cholesterol-laden derives from atherosclerosis research. We previously showed that, in tuberculosis, surprisingly accumulate triglycerides. Here, we utilized bacterial ( Mycobacterium tuberculosis ), fungal Cryptococcus neoformans and human papillary renal cell carcinoma (pRCC) models to address the need for a new explanation biogenesis. applied...
Protection from severe disease and hospitalization by SARS-CoV-2 vaccination has been amply demonstrated real-world data. However, the rapidly evolving pandemic raises new concerns. One pertains efficacy of adenoviral vector-based vaccines, particularly single-dose Ad26.COV2.S, relative to mRNA vaccines.
Acinetobacter baylyi is one of few Gram-negative bacteria capable accumulating storage lipids in the form triacylglycerides and wax esters, which makes it an attractive candidate for production lipophilic products, including biofuel precursors. Thioesterases play a significant dual role triacylglyceride ester biosynthesis by either providing or removing acyl-CoA from this pathway. Therefore, 4 different thioesterase genes were cloned ADP1 expressed Escherichia coli to investigate their...
The PAH1-encoded phosphatidate phosphatase (PAP) catalyzes the Mg2+ -dependent dephosphorylation of to produce diacylglycerol, which can be acylated form triacylglycerol (TAG). In model oleaginous yeast Yarrowia lipolytica, TAG is major lipid produced, and its biosynthesis requires a continuous supply provided by PAP reaction. However, regulation Pah1 has not been studied in detail Y. thus contribution this well understood. work, we examined PAH1-mediated activity abundance localization...
Abstract Phosphatidate (PA) phosphatase dephosphorylates the membrane phospholipid PA to diacylglycerol (DAG) that can be used for synthesis of storage lipid triacylglycerol (TAG). In Yarrowia lipolytica , TAG biosynthesis is induced during lipogenic phase, which results in accumulation this cells. The lipogenesis requires supply DAG, but source DAG not known Y. . study, regulation and its contribution was examined Lipogenesis triggered by growing cells high‐glucose media, whereas control...
While the biomarkers of COVID-19 severity have been thoroughly investigated, key biological dynamics associated with resolution are still insufficiently understood.
Abstract Monitoring the burden and spread of infection with new coronavirus SARS-CoV-2, whether within small communities or in large geographical settings, is paramount importance for public health purposes. Serology, which detects host antibody response to infection, most appropriate tool this task, since virus-derived markers are reliably detected during acute phase infection. Here we show that our ELISA protocol, based on binding Receptor Binding Domain (RBD) S1 subunit viral Spike...
ABSTRACT Monitoring antibody prevalence is a valuable tool to evaluate the burden of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in community, identify risk factors, and assess impact clinical public health intervention strategies. The SARS-CoV-2 children United States early 2022 was estimated by Centers for Disease Control Prevention be 74.2%, using seroprevalence from variety sources. A study New Jersey Department Health late 2022/early 2023 unvaccinated found lower...
Abstract Multisystem inflammatory syndrome in children (MIS-C) is a rare, severe complication of COVID-19 characterized by multi-lineage immune activation and multi-organ system involvement. While baseline immunophenotypes have been characterized, innate cell functionality genetic risk factors remain poorly understood. To address these knowledge gaps, we assessed responses with MIS-C (n = 33) relative to healthy controls 5). We found that Toll like receptor (TLR) signaling severely dampened...
Protection from severe disease and hospitalization by SARS-CoV-2 vaccination has been amply demonstrated real-world data. However, the rapidly evolving pandemic raises new concerns. One pertains efficacy of adenoviral vector-based vaccines, particularly single-dose Ad26.COV2.S, relative to mRNA vaccines. We investigated immunogenicity Ad26.COV2.S vaccines in 33 subjects vaccinated with either vaccine class five months earlier on average. After controlling for time since vaccination,...
Abstract Background SARS-CoV-2, the virus that causes COVID-19, has rapidly spread globally beginning in late 2019. Early areas impacted by this pandemic US include Essex County, New Jersey. Beyond understanding prevalence of active infections and deaths, it is important to understand true burden infection community, as indicated seroprevalence antibodies directed virus. Understanding spectrum disease key effectiveness primary prevention control measures design interventions against...
While the biomarkers of COVID-19 severity have been thoroughly investigated, key biological dynamics associated with resolution are still insufficiently understood. We report a case full severe due to convalescent plasma transfusion. Following transfusion, patient showed fever remission, improved respiratory status, and rapidly decreased viral burden in fluids SARS-CoV-2 RNAemia. Longitudinal unbiased proteomic analysis single-cell transcriptomics peripheral blood cells conducted prior at...
Abstract Since human infection with coronavirus SARS-CoV-2 was first detected in December 2019, we are still developing an understanding of the nature and duration protection against this infection. Most neutralizing antibodies, which a key component protective response infection, target Receptor Binding Domain (RBD) Spike glycoprotein critically prevent binding virus to host cell receptor, viral entry. Thus, it is vital importance monitor presence antibodies RBD-specific B cells that for...
Background: India's under-five mortality rates (U5MRs) contribute 15.5% to the global U5MR. This study examines relationship between socioeconomics, caste system, and U5MRs.Methods: A population-based cross-sectional approach was used analyze data from 1987–2021 National Family Health Surveys. Bayesian model averaging applied evaluate influence of socioeconomic factors social hierarchy on U5MRs. The cohort method incorporated explore U5MR trends among four groups over a three-decade period...
The PAH1 gene encodes for phosphatidate phosphatase (PAP), the enzyme that catalyzes dephosphorylation of phosphatidic acid to yield diacylglycerol and P i . PAP activity is essential biosynthesis storage lipid triacylglycerol (TAG) in several eukaryotes (e.g. Saccharomyces cerevisiae ). In Yarrowia lipolytica , TAG accumulates during lipogenesis responsible increase cellular content under these conditions. We hypothesized lipogenic conditions, lack Y. homolog (i.e. pah1 Δ) should result a...
The PAH 1 gene encodes phosphatidate phosphatase (PAP) which catalyzes the conversion of (PA) to diacylglycerol used for synthesis triacylglycerol (TAG). Previous research in our lab showed that lack Pah1 (i.e. pah1 Δ) Y. lipolytica causes a significant decrease cellular lipid content and TAG levels, while phospholipid levels are increased. However, it is unclear whether this phenotype due loss catalytic activity or some other function protein (e.g. transcriptional regulation). objective...
The PAH 1 gene encodes for phosphatidate phosphatase (PAP) that plays a vital role in lipid biosynthesis by catalyzing the conversion of (PA) to diacylglycerol (DAG) which is used synthesize triacylglycerol (TAG). Previous research has shown PAP activity elevated during growth media induce lipogenesis. In this work, PAH1 ‐encoded and protein levels were analyzed wild type pah1 Δ cells results showed increased time‐dependent manner, but Pah1 declined with time. Examination subcellular...