A5087302160- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Cancer Genomics and Diagnostics
- BRCA gene mutations in cancer
- Influenza Virus Research Studies
- Electrochemical sensors and biosensors
- interferon and immune responses
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
Monash University
2012-2024
Genomics (United Kingdom)
2024
Australian Regenerative Medicine Institute
2023
Monash Institute of Medical Research
2011-2016
CSIRO Health and Biosecurity
2015-2016
Since the first reports on isolation of pluripotent mouse embryonic stem (ES) cells 3 decades ago, there have been numerous attempts to derive ES cell lines from commercially important livestock species with limited success. The recent discovery that ectopic expression a handful cell-related genes was capable inducing pluripotency in rodents and primates provided novel approach derivation lines. We used this cattle demonstrated POU5F1 (also known as Oct4), SOX2, KLF4, c-MYC alone not...
Abstract Background Genome-wide association studies (GWAS) have identified > 200 loci associated with breast cancer risk. The majority of candidate causal variants are in non-coding regions and likely modulate risk by regulating gene expression. However, pinpointing the exact target association, identifying phenotype it mediates, is a major challenge interpretation translation GWAS. Results Here, we show that pooled CRISPR screens highly effective at GWAS genes defining phenotypes they...
Influenza A viruses (IAV) pose a constant threat to human and poultry health. Of particular interest are the infections caused by highly pathogenic avian influenza (HPAI) viruses, such as H5N1, which cause significant production issues. In response infection, cells activate immune mechanisms that lead increased interferon (IFN) production. To investigate how alterations in signaling pathway affect cellular infection chicken, we used CRISPR/Cas9 generate chicken cell line lacks functional...
Abstract The number of studies conducted about nonenzymatic electrochemical sensors has increased in recent years due to the development more stable and robust electrodes using noble metals. One key aspects for achieving high sensing performance including detection limit sensitivity is design electrode architecture. Herein, we report a new platform featuring ultrathin standing gold nanowires (AuNWs) hydrogen peroxide (H 2 O ). use AuNWs resulted an electron transfer efficiency higher active...
Primordial germ cells (PGCs) are the earliest identifiable and completely committed progenitors of female male gametes. They obvious targets for genome editing because they assure transmission desirable or introduced traits to future generations. PGCs established at stages embryo development difficult propagate in vitro--two characteristics that pose a problem their practical application. One alternative method enrich vitro is differentiate them from pluripotent stem derived adult tissues....
Pluripotent stem cells (PSCs) fall in two states, one highly undifferentiated, the naïve state, and primed characterized by inability to contribute germinal lineage. Several reports have demonstrated that these states can be modified changes cell culture conditions. With advent of nuclear reprogramming, bovine induced pluripotent (biPSCs) been generated. These represent examples a transient‐intermediate state pluripotency with remarkable characteristics biotechnological potential. Herein, we...
Exogenous expression of Oct4, Sox2, Klf4, and cMyc forces mammalian somatic cells to adopt molecular phenotypic characteristics embryonic stem cells, commencing with the required suppression lineage-associated genes (e.g., Thy1 in mouse). Although omitting from reprogramming cocktail minimizes risks uncontrolled proliferation, its exclusion results fold reductions efficiency. Thus, feasibility substituting transgene (non-integrative) recombinant “pTAT-mcMyc” protein delivery was assessed,...
Abstract DNA methylation is an epigenetic mark that plays a critical role in regulation of gene expression. methylase (DNMT) inhibitors, inhibit global methylation, and have been key tool studies healthy or disease conditions. A major bottleneck the lack tools to induce methylation. Here, we engineered CRISPR based approach, was initially designed, enable site specific Using synergistic activation mediator (SAM) system, unexpectedly found regardless targeted sequence any sgRNA induced...
Abstract DNA methylation is an epigenetic mark that plays a critical role in regulating gene expression. methyltransferase (DNMT) inhibitors, inhibit global and have been key tool studies of methylation. A major bottleneck the lack tools to induce Here, we engineered CRISPR based approach, initially designed, enable site-specific Using synergistic activation mediator (SAM) system, unexpectedly find regardless targeted sequence any sgRNA induces genome-wide We term this method SAM-DNMT3A show...
The DEAD box polypeptide 4, DDX4 or VASA, is a highly conserved gene that encodes putative RNA helicase with the motif (Asp-Glu-Ala-Asp). Although little known about its role in germ cell genesis, VASA one of earliest, specialised markers primordial (PGC) specification. Furthermore, this process specification has been recapitulated to some degree vitro using embryonic stem cells (ESC) and induced pluripotent (iPSC) mice humans, expression as criteria for differentiation sorting...
Abstract Genome-wide association studies (GWAS) have identified >200 loci associated with breast cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding regions and likely modulate risk by regulating gene expression. However, pinpointing the exact target identifying phenotype it mediates is a major challenge interpretation translation GWAS. Here, we used pooled CRISPR activation suppression screens to evaluate predicted GWAS genes, define phenotypes they...
Summary Genome-wide association studies (GWAS) have identified >200 loci associated with breast cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding regions and likely to modulate risk by regulating gene expression. We recently developed a scoring system, INQUISIT, predict genes at BC-risk loci. Here, we used pooled CRISPR activation suppression screens validate INQUISIT predictions, define the phenotypes they mediate. measured proliferation 2D, 3D,...