Abstract Membrane adenylyl cyclase AC8 is regulated by G proteins and calmodulin (CaM), mediating the crosstalk between cAMP pathway Ca 2+ signalling. Despite importance of in physiology, structural basis its regulation CaM not well defined. Here, we report 3.5 Å resolution cryo-EM structure bovine bound to stimulatory Gαs protein presence /CaM. The reveals architecture ordered domains small molecule activator forskolin. extracellular surface features a negatively charged pocket, potential...

Gastrointestinal (GI) dysfunction is common in the intensive care unit (ICU), although there no consensus on biomarkers of GI dysfunction. We aimed to evaluate ultrasound-based gastric antrum measurements and serum intestinal fatty acid-binding protein (IFABP) citrulline levels relation critically ill patients.Adult patients receiving enteral nutrition stayed for ICU ≥48 h was included. described using Dysfunction Score (GIDS). Gastric measurements, including craniocaudal (CC) diameter,...

Abstract Membrane adenylyl cyclase AC8 is regulated by G proteins and calmodulin (CaM), mediating the crosstalk between cAMP pathway Ca 2+ signalling. Despite importance of in physiology, including cognitive functions memory, structural basis its regulation CaM not well defined. Here we report 3.5 Å resolution cryo-EM structure bovine bound to /CaM stimulatory Gαs protein. The reveals architecture ordered domains a small molecule activator forskolin. extracellular surface features negatively...

In this study, molecularly imprinted polymer membranes were synthesized for the recognition and adsorption of quercetin. For this, quercetin polymeric [p(HEMA-MAH)] (Poly(2-hydroxyethyl methacrylate-co-N-methacryloly-l-histidinemethylester) by UV polymerization technique using HEMA MAH as monomers. Synthesized characterized with SEM, FTIR swelling test. Characterized used direct in a batch system. Quercetin conditions optimized membrane altering pH, temperature initial concentration medium....

The ongoing COVID-19 pandemic poses a significant threat to human health. Many hypotheses regarding pathogenesis have been proposed and are being tried be clarified by experimental clinical studies. This study aimed reveal the roles of innate immune system modulator GAS6/sAXL pathway, endothelial dysfunction markers vascular growth factor (VEGF) hypoxia-inducible (HIF)-1α, antiviral effective TRIM25 TRIM56 proteins in COVID-19. included 55 patients with 25 healthy individuals. serum levels...

<title>Abstract</title> <bold>Background:</bold>Idiopathic granulomatous mastitis (IGM) is an uncommon, benign, chronic inflammatory breast disease. There are several hypotheses about the etiology of IGM. The interaction between growth arrest-specific protein 6 (Gas6)/Tyro, AXL, and Mer (TAM) affects regulation innate immune reactions promotes induction phagocytosis a reduction in proinflammatory cytokine expression. We analyzed potential implications Gas6/sAXL signaling pathway, which...

Purpose: Behçet’s disease (BD) is an autoimmune chronic systemic inflammatory characterized by a versatile clinical spectrum. Growth arrest specific protein 6 (GAS6)/soluble AXL (sAXL) signaling pathway draws attention in the resolution of inflammation, and its deficiency associated with inflammatory, diseases, as well clearance apoptotic cells phagocytes – efferocytosis. In this study, it was aimed to investigate whether GAS6/sAXL, interleukin (IL)-10, nitric oxide (NO), BCL-2 levels were...

Abstract Objectives Cholangiocarcinoma (CCA) is a highly heterogeneous biliary malignant tumor. Studies have demonstrated that JAK/STAT signaling activated in many types of cancer. In addition, downstream AXL, and the AXL receptor by its ligand, GAS6. this study, we investigated anticarcinogenic effect silibinin relationship with GAS6/AXL ve pathway human EGI-1 cell line. Methods Cell viability, apoptosis, cycle were measured Muse Analyzer. All protein levels determined ELISA method. Results...

Abstract The current conditions in the progression of SARS-CoV2 pandemic changed scientific paradigm, and we now observe a novel rhythm way evaluating collected information. Previous experiences epidemics with similar viruses (viz., SARS-CoV1, MERS-CoV) information about viral transmission replication can be used to overcome pandemic. Although emerged very recently, there are plenty studies comment on situation. Inhibition spike protein activation, inhibition virus endocytosis, using soluble...

Abstract Objectives Cholangiocarcinoma (CCA) is usually diagnosed at a late stage due to resistance chemotherapeutic drugs. Epithelial mesenchymal transition (EMT) biological process in cancer that allows multiple biochemical changes enable epithelial cells acquire phenotype. In the present study, we focused on EMT which an important carcinogenesis and metastatic progression, also investigate effect of silibinin cell proliferation, colony formation, migration, apoptosis, cycle EMT. Methods...

Abstract Background Therapeutic options for advanced cholangiocarcinoma (CCA) are limited and ineffective due to the largely incomplete understanding of molecular pathogenesis this deadly tumor. So that, we planned investigate epigenetic regulation epithelial-mesenchymal transition (EMT) in cell line by applying Suberoylanilide hydroxamic acid (SAHA). We studied effect SAHA on proliferation, colony forming, migration protein level E-cadherin (E-cad) as an epithelial EMT marker, N-cadherin...

Although the molecular pathogenesis of hepatocellular carcinoma (HCC) is uncertain, it known that epithelial-mesenchymal transition (EMT) mechanism and epigenetic changes have an important role. This study was focused on evaluating relationship 3-Deazaneplanocin A (DZNep) with EMT mechanism, which a histone methyltransferase inhibitor HCC also as enhancer zeste homolog 2 (EZH2) inhibitor. Cell viability HepG2 cells (HCC cell line) assessed for DZNep over 72 hours...

Background &amp; Objective: Ischemic stroke is the leading cause of death and long-term disability worldwide. In patients with ischemic stroke, both cell loss inflammation are observed. GAS6/sAXL signaling effective in clearance dead/dying cells. This study investigated pathway its role acute stroke. Specifically, we evaluated whether was associated severity infarct volume. Methods: involved 53 (AIS) 49 healthy controls. GAS6 sAXL proteins were collected first 24 hours NIHSS scores, GCS,...

Abstract Objectives Hepatic fibrosis is a complex and dynamic process, such as “wound healing”. The effect of 3-deazaneplanocin A (DZNep) via enhancer zeste homolog 2 (EZH2) inhibition on transforming growth factor, matrix metalloproteinases 2-9 (MMP2, MMP9) inhibitor 3 (TIMP3), alpha-smooth muscle actin ( α -SMA), collagen 1A1(COL1A1), 3A1 (COL3A1) genes/proteins in human hepatic stellate cell line were examined. Inhibition EZH2 colony formation migration investigated. Methods 5 μM DZNep...

Abstract Background Hepatic fibrosis is a complex and dynamic process similar to “wound healing” that results in the progressive accumulation of connective tissue. We aimed investigate epigenetic control liver Hippo pathway human hepatic stellate cell (HSC) line. examined effect Suberoylanilide hydroxamic acid (SAHA), histone deacetylase inhibitor on LX2 Material methods 2.5 μM SAHA was treated line for 2 days. Cell proliferation apoptosis measurement were performed by Muse Analyzer....