A5068148062- Analytical chemistry methods development
- Chromium effects and bioremediation
- Forensic Toxicology and Drug Analysis
- Analytical Chemistry and Chromatography
- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Heavy Metal Exposure and Toxicity
- Potato Plant Research
- Mercury impact and mitigation studies
- Psychedelics and Drug Studies
- Cannabis and Cannabinoid Research
- Edible Oils Quality and Analysis
- Neurotransmitter Receptor Influence on Behavior
- Heavy metals in environment
- Aluminum toxicity and tolerance in plants and animals
- Gold and Silver Nanoparticles Synthesis and Applications
- Heavy Metals in Plants
- Graphene and Nanomaterials Applications
- Pesticide Residue Analysis and Safety
- Crop Yield and Soil Fertility
- Metabolomics and Mass Spectrometry Studies
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Electrochemical Analysis and Applications
- Banana Cultivation and Research
Universidade Federal dos Vales do Jequitinhonha e Mucuri
2023
Universidade do Porto
2007-2020
Rede de Química e Tecnologia
2008-2020
Universidade Federal de Viçosa
2019-2020
Fundação Oswaldo Cruz
2020
Polytechnic Institute of Bragança
2007
Universidade de Vigo
2007
Polytechnic Institute of Viana do Castelo
2006
ABSTRACT The toxicological profile of gold nanoparticles (AuNPs) remains controversial. Significant efforts to develop surface coatings improve biocompatibility have been carried out. In vivo biodistribution studies shown that the liver is a target for AuNPs accumulation. Therefore, we investigated effects induced by ~20 nm spherical (0–200 μM Au) with two coatings, citrate (Cit) compared 11‐mercaptoundecanoic acid (11‐MUA), in human HepG2 cells. Cytotoxicity was evaluated using...
Chromium is a controversial element with important essentiality and toxicity, depending on its different species; speciation analysis in principal human foodstuffs, as the case of bread, utmost importance. With this purpose, method was validated, including wet acid digestion procedure for total chromium dissolution, selective alkaline extraction hexavalent chromium, ETAAS determination. The applied to determination 152 bread samples. contents were 47.3 ± 20.0 50.9 22.2 μg/kg dry weight white...
Gold nanoparticles (AuNPs) have biomedical application on imaging and due to increased optical performance, star-shaped AuNPs are of special interest. Because shape, size capping greatly influence their toxicokinetics toxicodynamics, a systematic multiparametric comparative study the these parameters cytotoxicity, internalization, in vitro permeability was conducted human Cerebral Microvascular Endothelial Cell line (hCMEC/D3), an model blood-brain barrier (BBB). different (14 nm ∼50 nm),...
Amphetamine derivatives are a class of compounds increasingly abused as recreational drugs in various regions the world. Although d-amphetamine (AMPH) and 3,4-methylenedioxymethamphetamine (MDMA, ecstasy) among most commonly used, abuse other designer such 4-bromo-2,5-dimethoxyphenethylamine (2C-B) 4-methylthioamphetamine (4-MTA) their involvement acute intoxications has been reported. There is evidence that abusers ingest these either alone or combination respective monitoring important for...
A simple reverse-phase (RP) high performance liquid chromatography (HPLC) method was developed and validated, according to the International Harmonisation Guidelines (ICH), for determination of risperidone (RISP) from solid lipid nanoparticles (SLN). Chromatographic runs were performed on a RP-C18 column, using an isocratic mobile phase methanol, acetate buffer (0.05 M; pH 4.6) triethylamine (60:40:0.02, v/v/v). The flow rate 1 ml/min, run time 10 min RISP absorbance measured at 280 nm, UV...
Gold nanoparticles (AuNPs) are highly attractive to biomedical applications. Here, we investigated the effects of (i) ca. 15 nm spherical AuNPs capped with citrate or 11-mercaptoundecanoic acid (MUA) and (ii) 60 citrate-capped AuNPs, MUA-capped star-shaped on cytotoxicity, cellular uptake permeability, using media supplemented not 1% fetal bovine serum (FBS) caucasian colon adenocarcinoma Caco-2 cells. In addition, colloidal stability in (supplemented not) was assessed after 24 h-incubations...
An ETAAS method was validated to quantify total Cr and CrVI in mushrooms the underlying soils. The includes a sample pretreatment for dissolution using wet acid digestion procedure selective alkaline extraction CrVI. limits of detection were, expressed μg/L, 0.15 0.17 CrVI, respectively. linearity ranges under optimized conditions were 0.15−25.0 0.17−20.0 μg/L quantification μg/g dry weight, 0.0163 0.0085 hexavalent chromium, precision instrumental lower than 1.6%, analytical method, it 10%....
Gold nanoparticles (AuNPs) are promising nanoplatforms for drug therapy, diagnostic and imaging. However, biological comparison studies different types of AuNPs fail in consistency due to the lack sensitive methods detect subtle differences expression toxicity. Therefore, innovative approaches such as metabolomics much needed discriminate toxicity, specially at low doses. The current work aims compare vivo toxicological effects gold nanospheres versus nanostars (of similar ~40 nm diameter...
Abstract A comparative study to extract and purify total morphine in urine is described using several procedure treatments of biological samples. Sep-Pak C18 cartridges, Extrelut columns liquid-liquid conventional extraction with sequencial purification through column were assayed order establish the best quantitatively from free endogenous interferents. Analysis drug was made by HPLC-UV a methanol: acetate buffer (pH6.9) (70+30) as eluent. Recovery spiked 88.0% 79.8% for concentrations...
A survey of the presence total and hexavalent chromium in lager beers was conducted to understand variability between different styles beer packaged glass or cans estimate daily intake Cr from beer. Graphite-furnace atomic absorption spectroscopy using validated methodologies applied. Selective extraction performed a Chromabond NH2/500 mg column elution with nitric acid. The detection limits were 0.26 0.68 μg L–1 for Cr(VI), respectively. mean content ranged 1.13 canned pale 4.32 low-alcohol...
1 Abstract—The expansion of Digital Television and the convergence between conventional broadcasting television over IP contributed to gradual increase number available channels on demand video content. Moreover, dissemination use mobile devices like laptops, smartphones tablets everyday activities resulted in a shift traditional viewing paradigm from couch everywhere, anytime any device. Although this new scenario enables great improvement experiences, it also brings challenges given...
Procedures for the quantification of total chromium and hexavalent in UHT milk samples are presented. Total was determined directly with addition a surfactant mixture Pd Mg as chemical modifier. For selective separation chromium, sample pre-treatment consisted precipitation proteins elution supernatant through Chromabond NH2 column. The metal eluted nitric acid. Both were evaluated by atomic absorption spectrometry electrothermal atomization using same instrumental conditions. detection...
Accuracy and precision of silicon determination in biological matrices (serum, urine, water, beer spinach) by spectrometric techniques (when necessary after acid destruction) were assessed means a collaborative interlaboratory trial. The trial was set up accordance with ISO 5725-2 (1994). relative overall repeatability standard deviation acceptable. It varied between 4% for spinach powder (mean content: 176 mg kg−1) 11% serum 5.33 L−1). On the other hand, between-laboratory found to vary...
An electrothermal atomization atomic absorption spectrometry method was validated to quantify residues of copper, aluminum, cadmium, chromium, iron, lead, and nickel in olive fruit. The linearity ranges under the optimized conditions were 0.19−20.0, 1.11−50.0, 0.02−2.0, 0.15−20.0, 0.80−20.0, 0.35−50.0, 0.60−50.0 μg/L, respectively. limits quantification were, expressed nanograms per gram dry weight, 12.6, 74.0, 1.34, 10.0, 53.4, 23.4, 40.0, For all metals precision instrumental <6.3% that...