A5065945117- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Supramolecular Self-Assembly in Materials
- Sulfur-Based Synthesis Techniques
- Click Chemistry and Applications
- Biotin and Related Studies
- Amino Acid Enzymes and Metabolism
- Molecular Junctions and Nanostructures
- Transplantation: Methods and Outcomes
- Catalytic C–H Functionalization Methods
- Immunodeficiency and Autoimmune Disorders
- Chemical Synthesis and Reactions
- Organic Chemistry Cycloaddition Reactions
- Participatory Visual Research Methods
- Polymer Surface Interaction Studies
- Wave and Wind Energy Systems
- Cognitive Science and Mapping
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nuclear Receptors and Signaling
- Macrophage Migration Inhibitory Factor
- COVID-19 and healthcare impacts
- ATP Synthase and ATPases Research
- Anesthesia and Pain Management
- Historical Geography and Geographical Thought
McGill University
2023-2025
Université Grenoble Alpes
2025
Centre National de la Recherche Scientifique
2025
University of Geneva
2019-2022
Inserm
2022
Laboratoire de Recherche Vasculaire Translationnelle
2022
NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
2019-2021
École Polytechnique Fédérale de Lausanne
2018-2021
Université de Versailles Saint-Quentin-en-Yvelines
2021
Hôpital Foch
2021
ConspectusStructural DNA nanotechnology offers a unique self-assembly toolbox to construct soft materials of arbitrary complexity, through bottom-up approaches including origami, brick, wireframe, and tile-based assemblies. This can be expanded by incorporating interactions orthogonal base-pairing such as metal coordination, small molecule hydrogen bonding, π-stacking, fluorophilic interactions, or the hydrophobic effect. These allow for hierarchical long-range organization in supramolecular...
Oligonucleotide phosphorothioates (OPS) are DNA or RNA mimics where one phosphate oxygen is replaced by a sulfur atom. They have been shown to enter mammalian cells much more efficiently than non-modified DNA. Thus, solving of the key challenges with oligonucleotide technology, OPS became very useful in practice, several FDA-approved drugs on market late clinical trials. However, mechanism accounting for this facile cellular uptake unknown. Here, we show that thiol-mediated uptake. The...
Siderophores are iron chelators secreted by bacteria to scavenge iron(III) from their surrounding environment. They possess own internalization pathway that is sufficiently unselective be hijacked, making them suitable for Trojan Horse strategy applications. A commercially available siderophore, deferoxamine B (DFO), was derivatized at the primary amine with carboxylic acids bearing different ligands afford mono- and bidentate complexes ruthenium as well a RAPTA-like complex in which DFO...
Preoperative COVID-19 has been associated with excess postoperative morbi-mortality. Consequently, guidelines were developed that recommended the postponement of surgery for at least 7 weeks after infection. We hypothesised vaccination against SARS-CoV-2 and large predominance Omicron variant attenuated effect a preoperative on occurrence respiratory morbidity.We conducted prospective cohort study in 41 French centres between 15 March 30 May 2022 (ClinicalTrials NCT05336110), aimed comparing...
Abstract Oligonucleotide therapeutics, including antisense oligonucleotides and small interfering RNA, offer promising avenues for modulating the expression of disease‐associated proteins. However, challenges such as nuclease degradation, poor cellular uptake, unspecific targeting hinder their application. To overcome these obstacles, spherical nucleic acids have emerged versatile tools acid delivery in biomedical applications. Our laboratory has introduced sequence‐defined DNA amphiphiles...
In this report, cell-penetrating streptavidin (CPS) is introduced to exploit the full power of streptavidin–biotin biotechnology in cellular uptake. For purpose, transporters, here cyclic oligochalcogenides (COCs), are covalently attached lysines wild-type streptavidin. This leaves all four biotin binding sites free for at least bifunctional delivery. To maximize standards quantitative evaluation cytosolic delivery, recent chloroalkane penetration assay (CAPA) coupled with automated high...
Abstract The thiol‐mediated opening of 3‐alkyl‐1,2‐dithiolanes and diselenolanes is described. thiolate nucleophile shown to react specifically with the secondary chalcogen atom, against steric demand, probably because primary atom provides a better leaving group. Once released, this reacts obtained dichalcogenide produce constitutional isomer. Thiolate migration equilibrates within ca . 20 ms at room temperature 3 : 2 ratio in favor dichalcogenide. clarification focused question important...
The cytosolic delivery of various substrates in 3D multicellular spheroids by thiol-mediated uptake is reported. This important because most orthodox systems, including polycationic cell-penetrating peptides, fail to deliver efficiently into deep tissue. grand principles supramolecular chemistry, that the pH dependence dynamic covalent disulfide exchange with known thiols on transferrin receptor, are proposed account for transcytosis tissue, while but elusive cascades along same or other...
Cyclic oligochalcogenides are emerging as powerful tools to penetrate cells. With disulfide ring tension maximized, selenium chemistry had be explored next enhance speed and selectivity of dynamic covalent exchange on the way into cytosol. We show that diseleno lipoic acid (DiSeL) delivers a variety relevant substrates. DiSeL-driven uptake artificial metalloenzymes enables bioorthogonal fluorophore uncaging within Binding bicyclic peptide, phalloidin, actin fibers evinces targeted delivery...
Abstract Nucleic acid therapeutics (NATs), such as mRNA, small interfering RNA or antisense oligonucleotides are extremely efficient tools to modulate gene expression and tackle otherwise undruggable diseases. Spherical nucleic acids (SNAs) can efficiently deliver NATs cells while protecting their payload from nucleases, have improved biodistribution muted immune activation. Self‐assembled SNAs emerged nanostructures made a single DNA‐polymer conjugate with similar favorable properties well...
A model system is introduced as a general tool to elaborate on orthogonal templation of dynamic covalent ring-opening polymerization (ODC-TROP). The consists 3
A novel route to medicinally-relevant dihydrobenzofurans utilises a sulfur-directed C-H ortho-coupling of arenes and unactivated terminal alkenes mediated by iron, palladium-catalysed deallylation/heterocyclisation sequence. The iron-mediated coupling affords linear products alkene chloroarylation in good yield with complete regioselectivity. likely proceeds redox-activation the arene partner iron(iii) addition resultant radical cation.
Supramolecular materials have been assembled using a wide range of interactions, including the hydrophobic effect, DNA base-pairing, and hydrogen bonding. Specifically, amphiphiles with building block self-assemble into diverse morphologies depending on length composition both blocks. Herein, we take advantage orthogonality different supramolecular interactions - Watson-Crick-Franklin base pairing RNA kissing loops to create hierarchical self-assemblies controlled nanometer micrometer...
Les géographes ont depuis le début du 20ème siècle recours à l’usage de l’audiovisuel, qu’il s’agisse d’analyser ou produire des films dans cadre recherches. Ils donc dû se former aux outils et méthodes l’image animée son. L’article propose ainsi revenir sur l’histoire qui utilisé la vidéo leur recherche avant présenter l’apport l’audiovisuel en géographie. Il termine une présentation formation proposée par l’Université Paris 1 étudiants géographie ce domaine.
Abstract Cellular uptake mediated by cyclic oligochalcogenides (COCs) is emerging as a conceptually innovative method to penetrate mammalian cells. Their mode of action based on dynamic covalent oligochalcogenide exchange with cellular thiols. To test thiol‐mediated in bacteria, five antibiotics have been equipped up three different COCs: One diselenolane and two dithiolanes. We found that the COCs do not activate Gram‐negative bacteria. In Gram‐positive inactivate act cytoplasm reduce...
Coagulation factor XI (FXI) has emerged as a promising target for the development of safer anticoagulation drugs that limit risk severe and life-threatening bleeding. Herein, we report first cyclic peptide-based FXI inhibitor selectively potently inhibits activated (FXIa) in human animal blood. The peptide (Ki = 2.8 ± 0.5 nM) achieved effects are comparable to gold standard heparin applied at therapeutic dose (0.3–0.7 IU/mL plasma) but with substantially broader estimated range. We extended...
In this paper, we describe the precise synthesis of poly(phosphodiesters) with up to four monomers. These self-assemble into various nanostructures where a single monomer difference drastically impacts structure's size and morphology.
Abstract We evaluated the contribution of artificial intelligence in predicting risk acute cellular rejection (ACR) using early plasma levels soluble CD31 (sCD31) combination with recipient haematosis, which was measured by ratio arterial oxygen partial pressure to fractional inspired (PaO 2 /FiO ) and respiratory SOFA (Sequential Organ Failure Assessment) within 3 days lung transplantation (LTx). is expressed on endothelial cells, leukocytes platelets acts as a “peace-maker” at blood/vessel...
Abstract Oligonucleotide phosphorothioates (OPS) are DNA or RNA mimics where one phosphate oxygen is replaced by a sulfur atom. They have been shown to enter mammalian cells much more efficiently than non‐modified DNA. Thus, solving of the key challenges with oligonucleotide technology, OPS became very useful in practice, several FDA‐approved drugs on market late clinical trials. However, mechanism accounting for this facile cellular uptake unknown. Here, we show that thiol‐mediated uptake....
Nucleic acids, used as therapeutics to silence disease‐related genes, offer significant advantages over small molecule drugs: they provide high specificity, the ability target "undruggable" molecules, and adaptability a wide range of disease phenotypes. However, their instability in biological media, well rapid clearance from organism limit applicability, necessitating use nanocarriers overcome these challenges. Among strategies, spherical nucleic acids (SNA) – composed densely packed corona...
Nucleic acids, used as therapeutics to silence disease‐related genes, offer significant advantages over small molecule drugs: they provide high specificity, the ability target "undruggable" molecules, and adaptability a wide range of disease phenotypes. However, their instability in biological media, well rapid clearance from organism limit applicability, necessitating use nanocarriers overcome these challenges. Among strategies, spherical nucleic acids (SNA) – composed densely packed corona...
Abstract Nucleic acid therapeutics (NATs), such as mRNA, small interfering RNA or antisense oligonucleotides are extremely efficient tools to modulate gene expression and tackle otherwise undruggable diseases. Spherical nucleic acids (SNAs) can efficiently deliver NATs cells while protecting their payload from nucleases, have improved biodistribution muted immune activation. Self‐assembled SNAs emerged nanostructures made a single DNA‐polymer conjugate with similar favorable properties well...