A5010518781- Metal complexes synthesis and properties
- Porphyrin Metabolism and Disorders
- Bacterial Genetics and Biotechnology
- Sphingolipid Metabolism and Signaling
- RNA and protein synthesis mechanisms
- Plant-based Medicinal Research
- Enzyme Structure and Function
- Neurological diseases and metabolism
- Lipid Membrane Structure and Behavior
- RNA regulation and disease
- Bacteriophages and microbial interactions
- Microtubule and mitosis dynamics
- Amyotrophic Lateral Sclerosis Research
- Biological Activity of Diterpenoids and Biflavonoids
- Peptidase Inhibition and Analysis
- Blood disorders and treatments
- Escherichia coli research studies
- Polar Research and Ecology
- Sulfur Compounds in Biology
- Pesticide and Herbicide Environmental Studies
- Luminescence and Fluorescent Materials
- Metalloenzymes and iron-sulfur proteins
- Metal-Catalyzed Oxygenation Mechanisms
- Polyoxometalates: Synthesis and Applications
- Phytochemicals and Antioxidant Activities
Yale University
2025
Southern University of Science and Technology
2022-2023
Chinese Academy of Sciences
2022-2023
Shanghai Institute of Materia Medica
2023
Hefei National Center for Physical Sciences at Nanoscale
2020-2022
University of Science and Technology of China
2019-2022
Xinjiang Institute of Ecology and Geography
2022
Sichuan University of Science and Engineering
2020-2022
Kunming Institute of Botany
1996-1998
Abstract The ORM/ORMDL family proteins function as regulatory subunits of the serine palmitoyltransferase (SPT) complex, which is initiating and rate-limiting enzyme in sphingolipid biosynthesis. This complex tightly regulated by cellular levels, but sensing mechanism unknown. Here we show that purified human SPT-ORMDL complexes are inhibited central metabolite ceramide. We have solved cryo-EM structure SPT-ORMDL3 a ceramide-bound state. Structure-guided mutational analyses reveal essential...
The serine palmitoyltransferase (SPT) complex catalyzes the first and rate-limiting step in sphingolipid biosynthesis all eukaryotes. ORM/ORMDL proteins are negative regulators of SPT that respond to cellular levels. However, molecular basis underlying ORM/ORMDL-dependent homeostatic regulation is not well understood. We determined cryo-electron microscopy structure Arabidopsis SPT-ORM1 complex, composed LCB1, LCB2a, SPTssa, ORM1, an inhibited state. A ceramide molecule sandwiched between...
Spirochetes are a widely existing group of bacteria with distinct morphology. Some spirochetes important human pathogens that utilize periplasmic flagella to achieve motility and host infection. The motors drive the rotation have unique spirochete-specific feature, termed collar, crucial for flat-wave morphology Lyme disease spirochete Borrelia burgdorferi . Here, we deploy cryo-electron tomography subtomogram averaging determine high-resolution in-situ structures B flagellar motor....
The adenosine 5′-triphosphate (ATP)–binding cassette (ABC) transporter ABCA3 plays a critical role in pulmonary surfactant biogenesis. Mutations human have been recognized as the most frequent causes of inherited dysfunction disorders. Despite two decades research, vitro biochemical and structural studies are still lacking. Here, we report cryo-EM structures distinct conformations, both at resolution 3.3 Å. In absence ATP, adopts “lateral-opening” conformation with lateral surfaces...
YoeB-YefM, the widespread type II toxin-antitoxin (TA) module, binds to its own promoter autoregulate transcription: repress or induce transcription under normal stress conditions, respectively. It remains unclear how YoeB-YefM regulates depending on YoeB YefM TA ratio. We find that complex from S.aureus exists as two distinct oligomeric assemblies: heterotetramer (YoeB-YefM2-YoeB) and heterohexamer (YoeB-YefM2-YefM2-YoeB) with low high DNA-binding affinities, Structures of alone bound DNA...
Three novel binuclear copper(<sc>ii</sc>) complexes with reduced <sc>l</sc>-serine Schiff bases exhibited significant activity differences for the hydrolysis of PNPA and PNPP, suggesting a favourable intramolecular nucleophilic pathway PNPP.
Oxidative cleavage of phosphate diesters (HPNP, BNPP) is highly faster than the hydrolytic one by binuclear metal complexes with novel imidazole derivative, producing a non-lactone monoester due to direct attack free radicals.
Toxin-antitoxin (TA) systems are ubiquitous regulatory modules for bacterial growth and cell survival following stress. YefM-YoeB, the most prevalent type II TA system, is present in a variety of species. In Staphylococcus aureus, YefM-YoeB system exists as two independent paralogous copies. Our previous research resolved crystal structures oligomeric states (heterotetramer heterohexamer-DNA ternary complex) first paralog well molecular mechanism transcriptional autoregulation this module....
Gene expression is mediated by a series of regulatory proteins, i.e., transcription factors. Under different growth conditions, the transcriptional regulation structural genes associated with recognition specific elements (REs) in promoter DNA. The manner which factors recognize distinctive REs key question biology. Previous research has demonstrated that Ino2p/Ino4p heterodimer phospholipid biosynthetic genes. Mechanistically, could specifically inositol/choline-responsive element (ICRE),...
Anthranilate phosphoribosyltransferase (AnPRT) catalyzes the transfer of phosphoribosyl group 5'-phosphoribosyl-1'-pyrophosphate (PRPP) to anthranilate form phosphoribosyl-anthranilate. Crystal structures AnPRTs from bacteria and archaea have previously been determined; however, structure Saccharomyces cerevisiae AnPRT (ScAnPRT) still remains unsolved. Here, crystal ScAnPRT in apo as well complex with its substrate PRPP analogue 4-fluoroanthranilate (4FA) are presented. These demonstrate...
TYE7, a bHLH (basic helix–loop–helix) transcription factor from Saccharomyces cerevisiae , is involved in the regulation of many genes, including glycolytic genes. Meanwhile, accumulating evidence indicates that TYE7 also functions as cyclin and linked to sulfur metabolism. Here, structure (residues 165–291) complexed with its specific DNA was determined by X-ray crystallography. Structural analysis comparison revealed His185 Glu189 are conserved base recognition. However, Arg193 recognition...
Atranorin 1, Usnic acid 2, and D-Arabinitol 3 were isolated from Usnea aciculifera by extensive column chromatography. The structures elucidated based on the comprehensive spectral analysis including FT IR, GC MS, ESI 13C NMR, 1H NMR. compounds evaluated for their cytotoxic activity against LN-229 glioblastoma cancer cell line performing Sulforhodamine B assay (SRB). 2 showed strong with an IC50 value of 3.09 μg/mL.
Three depsides, namely sekikaic acid (1), homosekikaic (2), and 4′-O-demethylsekikaic (3) were isolated from acetone fraction of Ramalina spp. The structures characterized by using spectroscopic techniques like 1H NMR, 13C ESI MS IR. quantitative determinations these compounds conducted reversed phase high performance liquid chromatography with photodiode array detector (LC-PDA). developed assay method could be considered as a suitable quality control for content on percentage dry basis...
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