A5027377475- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Immune cells in cancer
- Cancer, Stress, Anesthesia, and Immune Response
- Neuroscience and Neural Engineering
- Graphene and Nanomaterials Applications
- CAR-T cell therapy research
- Nerve injury and regeneration
- Nanoplatforms for cancer theranostics
- Acute Myeloid Leukemia Research
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Advanced Memory and Neural Computing
- Anesthesia and Neurotoxicity Research
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Spinal Cord Injury Research
- Bone Tissue Engineering Materials
- Characterization and Applications of Magnetic Nanoparticles
- Conducting polymers and applications
- Chemokine receptors and signaling
- S100 Proteins and Annexins
Consejo Superior de Investigaciones Científicas
2017-2025
Instituto de Ciencia de Materiales de Madrid
2018-2024
UCLouvain
2024
Moffitt Cancer Center
2024
The University of Texas MD Anderson Cancer Center
2020-2022
Melanoma Institute Australia
2022
Servicio de Salud de Castilla La Mancha
2017-2021
Instituto de Ciencia de Materiales de Sevilla
2019
Instituto Cajal
2017
Mayo Clinic in Arizona
2008-2016
Abstract In our previous in vivo study we demonstrated that young BALB/c mice effectively rejected the BM-185 tumor cells expressing enhanced GFP (EGFP) as a surrogate Ag. contrast, old succumbed to BM-185-EGFP tumors, indicating there is deficiency animals preventing rejection of immunogenic tumors. There cumulative evidence regulatory T (Treg) control activation primary and memory cell responses. However, very little known about whether relation between Tregs lack immune responses aged. We...
ABSTRACT Vaccination is the most efficient prophylaxis against a variety of infectious diseases. New vaccination strategies rely on incorporation effective adjuvants, which stimulate innate immune response and, in turn, activate adaptive response. It well established that flagellin induces inflammatory responses through activation antigen-presenting cells (APCs). In order to evaluate whether can serve as carrier for development adjuvants or vaccines, we prepared flagellin-enhanced green...
Intratumoral accumulation of T regulatory cells (Tregs) creates an immunosuppressive environment that reduces the efficacy antitumor immunotherapy. The milieu within tumors is largely brought about by presence Tregs, which maintain self-tolerance directly inhibiting cells, NK and dendritic cells. Depletion Tregs enhances immune responses; however, current depletion therapies also affect function CD4 CD8 effector Previous studies from our laboratory indicate intratumoral delivery CpG-ODN...
Graphene oxide (GO) assists a diverse set of promising routes to build bioactive neural microenvironments by easily interacting with other biomaterials enhance their bulk features or, alternatively, self-assembling toward the construction biocompatible systems specific three-dimensional (3D) geometries. Herein, we first modulate both size and available oxygen groups in GO nanosheets adjust physicochemical biological properties polycaprolactone–gelatin electrospun nanofibrous systems. The...
The inability of CD8+ effector T cells (Teffs) to reach tumor is an important aspect resistance cancer immunotherapy. recruitment these the microenvironment (TME) regulated by integrins, a family adhesion molecules that are expressed on cells. Here, we show 7HP349, small-molecule activator lymphocyte function-associated antigen-1 (LFA-1) and very late activation antigen-4 (VLA-4) integrin cell-adhesion receptors, facilitated preferential localization tumor-specific improved antitumor...
Abstract Tumor cells express tumor‐associated antigens (TAAs), which can serve as targets for the immune system. However, majority of TAAs are overexpressed products normal cellular genes; such, self‐tolerance mechanisms have hindered their use induction effective antitumor responses. One such self‐protein is growth factor receptor Her‐2/neu, in 25–35% all mammary carcinomas humans. In previous studies, we demonstrated that Her‐2/neu mice functionally tolerant to neu and contain only a low...
Regulatory T cells (T reg cells) are characterized by the expression of forkhead lineage-specific transcription factor Foxp3, and their main function is to suppress cells. While evaluating cells, we identified a population Foxp3-positive that were CD11b(+)F4/80(+)CD68(+), indicating macrophage origin. These observed in spleen, lymph nodes, bone marrow, thymus, liver, other tissues naive animals. To characterize this subpopulation macrophages, devised strategy purify CD11b(+)F4/80(+)Foxp3(+)...
We have developed a reproducible and facile one step strategy for the synthesis of doxorubicin loaded magnetoliposomes by using thin-layer evaporation method. Liposomes around 200 nm were made 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) iron oxide nanoparticles (NPs) with negative, positive, hydrophobic surfaces that incorporated outside, inside, or between lipid bilayers, respectively. To characterize how NPs are in liposomes, advanced cryoTEM atomic force microscope (AFM) techniques...
Background The correlation between elevated T-cell infiltration and improved survival of ovarian cancer (OvCa) patients suggests that endogenous tumor-infiltrating lymphocytes (TIL) possess some degree antitumor activity can be harnessed for OvCa immunotherapy. We previously optimized a protocol ex vivo TIL expansion adoptive cell therapy, which is now being tested in clinical trial at our institution ( NCT03610490 ). Building on this success, we embarked genetic modification to overcome key...
The tolerogenic cytokine IL9 promotes T regulatory cell function and allergic airway inflammation, but it has not been extensively studied in cancer. In this report, we used IL9-deficient mice to investigate the effects of multiple models breast colon cancer development. Eliminating endogenous enabled sensitization host cells tumors, leading their early rejection without requirement vaccines or immunomodulatory therapies. Notably, acquired immunologic memory, which actively protected from...
Abstract The majority of tumor‐associated antigens are aberrantly expressed or overexpressed normal gene products. Therefore, mechanisms responsible for self tolerance dampen immune responses against these antigens. To evaluate the effect that has on tumor antigens, we characterized CD8 + T cell in neu mice. theA2.1/neu p369–377 and p773–782 peptides were evaluated mice crossed with A2.1/Kb transgenic (A2×neu). Tetramer binding cytotoxic activity demonstrate that, compared to CTL from...
Abstract There is a clear decrease in CD8+ T cell effector function with aging, loss once thought to be intrinsic the cells. Recent studies suggest, however, that this decline may consequence of altered stimulatory signals within aged lymphoid microenvironment. In study, we compared immune responses young and old mice against BM-185 pre-B lymphoma expressing enhanced GFP (EGFP) as surrogate tumor Ag. Young animals develop protective when immunized BM-185-EGFP, but do not ultimately succumb...
T cell responses are compromised in the elderly. The B7-CD28 family receptors critical regulation of immune responses. We evaluated whether B7-family and CD28-family were differentially expressed dendritic cells, macrophages, CD4(+) CD8(+) cells from young old mice, which could contribute to dysfunction old. Although most equally all >85% naive B7-H1 compared with 25% young. Considering that negatively regulates responses, we hypothesized expression would downregulate function cells. Old...
// Raffaella Pippa 1 , Ana Dominguez Raquel Malumbres Akinori Endo 2 Elena Arriazu 1, 3 Nerea Marcotegui Elizabeth Guruceaga and María D. Odero 3, 4 Hematology/Oncology Program, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain Centre Gene Regulation Expression, College Life Sciences, Dundee, UK Instituto de Investigación Sanitaria Navarra (IdiSNA), Department Biochemistry Genetics, Correspondence to: Odero, email: modero@unav.es Pippa, raffa80@gmail.com...
Spinal cord injury (SCI) is characterized by the disruption of neuronal axons and creation an inhibitory environment for spinal tissue regeneration. For decades, researchers clinicians have been devoting a great effort to develop novel therapeutic approaches which include fabrication biocompatible implants that could guide neural repair in lesion site attempt recover functionality nervous tissue. In this context, although fiberlike structures hypothesized serve as topographical guidance...
Abstract Although immunotherapy has achieved impressive durable clinical responses, many cancers respond only temporarily or not at all to immunotherapy. To find novel, targetable mechanisms of resistance immunotherapy, patient-derived melanoma cell lines were transduced with 576 open reading frames, exposed arrayed libraries 850 bioactive compounds, prior co-culture autologous tumor-infiltrating lymphocytes (TILs). The synergy between the targets and TILs induce apoptosis, inhibiting...
We present the design, fabrication, and characterization of an implantable neural interface based on anisotropic magnetoresistive (AMR) magnetic-field sensors that combine reduced size high performance at body temperature. The are La0.67Sr0.33MnO3 (LSMO) as a ferromagnetic material, whose epitaxial growth has been suitably engineered to get uniaxial anisotropy large AMR output together with low noise even frequencies. LSMO different film thickness temperatures close 37 °C be explored find...
Summary It is well established that immune responses are diminished in the old. However, we still do not have a clear understanding of what dictates dysfunction old T cells at molecular level. Although microarray analysis has been used to compare young and cells, identifying hundreds genes differentially expressed among these populations, it difficult utilize this information pinpoint which biological pathways truly affect function aged cells. To better define differences between naïve CD4 +...
Neuronal polarization underlies the ability of neurons to integrate and transmit information. This process begins early in development with axon outgrowth, followed by dendritic growth subsequent maturation. In between these two steps, initial segment (AIS), a subcellular domain crucial for generating action potentials maintaining morphological functional polarization, starts develop. However, cellular/molecular mechanisms receptors involved AIS maturation are mostly unknown. this study, we...