A5068154276- Renal Transplantation Outcomes and Treatments
- Organ Donation and Transplantation
- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Palliative Care and End-of-Life Issues
- Hepatitis C virus research
- Renal and Vascular Pathologies
- Pneumocystis jirovecii pneumonia detection and treatment
- Transplantation: Methods and Outcomes
- Urological Disorders and Treatments
- Grief, Bereavement, and Mental Health
- HIV/AIDS drug development and treatment
- Neurological Complications and Syndromes
- Blood donation and transfusion practices
- Hernia repair and management
- Organ and Tissue Transplantation Research
- Renal Diseases and Glomerulopathies
- Immune Cell Function and Interaction
- Adenosine and Purinergic Signaling
- Pharmacological Effects and Toxicity Studies
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- Complement system in diseases
- Geriatric Care and Nursing Homes
- Urinary and Genital Oncology Studies
Yale University
2015-2024
Kulkarni reconstructive Urology Center
2024
European Association of Urology
2024
New England Organ Bank
2020-2022
Yale New Haven Hospital
2006-2021
Indo-American Center
2020
University of Illinois Chicago
2000-2020
Yale Cancer Center
2011-2019
University of Cincinnati Medical Center
2019
Sabin Vaccine Institute
2019
Two independent approaches to understanding the molecular mechanism of cytokinesis have converged on gene spaghetti-squash (sqh). A genetic screen for mitotic mutants identified sqh1, a mutation that disrupts cytokinesis, which was then cloned by transposon tagging. Independently, encodes regulatory light chain biochemically defined nonmuscle myosin (MRLC-C) also cloned. We show here sqh MRLC-C and in sqh1 mutants, level stable transcript is greatly reduced. Reversion excision or...
Cardiac allograft vasculopathy is the major cause of late loss after heart transplantation. lesions contain alloreactive T cells that secrete interferon-γ, a vasculopathic cytokine, and occur more frequently in patients with donor-specific antibody. Pathological interactions between these immune effectors, representing cellular humoral immunity, respectively, remain largely unexplored.We used human panel reactive antibody to form membrane attack complexes on allogeneic endothelial vitro...
We hypothesized that de novo donor-specific antibody (DSA) causes complement-dependent endothelial cell injury in kidney transplants, as assessed by expression of cell-associated transcripts (ENDATs), may be attenuated through complement inhibition. In total, 15 participants (five control, 10 treatment) with DSA and deteriorating renal function were enrolled. The treatment group received 6 mo eculizumab followed observation, whereas controls observed. primary end point was percentage change...
ABSTRACT The X-linked Drosophila gene spaghetti squash (sqh) encodes the regulatory light chain of nonmuscle myosin II. To assess requirement for II in oogenesis and early embryogenesis, we induced homozygous germline clones hypomorphic mutation sqh1 otherwise het-erozygous mothers. Developing oocytes such often failed to attain full size due a defect ‘dumping’, rapid phase cytoplasmic transport from nurse cells. In contrast other dumpless mutants described date, egg chambers showed no...
Background1-year data from this trial showed the noninferiority of a novel once-daily extended-release tacrolimus (LCPT; Envarsus XR) to immediate-release (IR-Tac) twice daily after kidney transplantation.Study DesignFinal 24-month analysis 2-armed, parallel-group, randomized, double-blind, double-dummy, multicenter, phase 3 trial.Setting & Participants543 de novo recipients randomly assigned LCPT (n = 268) or IR-Tac 275); 507 (93.4%) completed study.InterventionLCPT tablets once at 0.17...
IMPORTANCEInactive patients on the kidney transplant wait-list have a higher mortality.The implications of this status change outcomes between racial/ethnic groups are unknown.OBJECTIVES To determine if activity changes differ among races/ethnicities and levels sensitization, these differences associated with probability after implementation Kidney Allocation System. DESIGN, SETTING, AND PARTICIPANTSA multistate model was constructed from Organ Procurement Transplantation Network database...
Human graft endothelial cells (ECs) can act as antigen-presenting to initiate allograft rejection by host memory T cells. Rapamycin, an mTOR inhibitor used clinically suppress cell responses, also acts on DCs, rendering them tolerogenic. Here, we report the effects of rapamycin EC alloimmunogenicity. Compared with mock-treated cells, rapamycin-pretreated human ECs (rapa-ECs) stimulated less proliferation and cytokine secretion from allogeneic CD4+ effect mimicked shRNA knockdown or raptor in...
Animal models and observational human data indicate that complement, including C5a, pathogenically participates in ischemia reperfusion (IR) injury manifests as delayed graft function (DGF) following deceased donor kidney transplantation. We report on the safety/efficacy of anti-C5 monoclonal antibody eculizumab (Ecu) administered operating room prior to reperfusion, prevent DGF recipients transplants two related, investigator-sponsored, randomized controlled trials. Eight from a single...
Abstract Perioperative injuries to an allograft exacerbate graft rejection, which in humans is primarily mediated by effector memory T cells. IL-6 transcripts human coronary artery segments rapidly increase posttransplantation into immunodeficient mouse hosts compared with those of pretransplant specimens and fall dramatically 30 d. Adoptive transfer PBMCs allogeneic the 2 d postoperatively results cell infiltrates intimal expansion 4 wk later. Ab neutralization reduces magnitude total...
The use of technically variant segmental grafts are key in offering transplantation to increase organ availability.To describe the allograft current era donor scarcity, minimizing vascular complications using innovative surgical techniques.Retrospective study from August 2007 2012 at a university hospital. A total 218 consecutive liver transplant patients were reviewed, and 69 (31.6%; 38 males 31 females; mean age, 22.5 years) received living donors or split/reduced-size deceased...
Despite effective antiviral treatment, hundreds of kidneys from deceased donors with hepatitis C virus (HCV) are discarded annually. Little is known about the determinants willingness to accept HCV-infected among HCV-negative patients.At 2 centers, 189 patients undergoing initial or reevaluation for transplant made 12 hypothetical decisions accepting in which we systematically varied expected HCV cure rate, allograft quality, and wait time an uninfected kidney.Only 29% participants would...
Living kidney donors are often excluded from the shared decision making and patient-centered models that advocated in medical practice. Thresholds for acceptable risk vary between transplant centers, clinicians donors. Although donor selection committees commonly focus on risks, potential also consider nonmedical risks burdens, which may alter their assessment of an level risk. Thus, centers encounter ethical tensions nonmaleficence respect autonomy. A donor-centered model reconciliation...
Background. Living kidney donation rates are increasing in the United States and internationally. Major consensus statements on care of living donors recommend communicating all potential health psychosocial risks to donors. We evaluated degree international variation process informed consent during their evaluation. Methods. Transplant professionals attending 2006 World Congress responded a survey assessing consent, evaluation communication donor risk. US-based respondents were compared...
Radiofrequency ablation (RFA) is commonly used for treating unresectable hepatic malignancies. Some associated complications of RFA include fever, symptomatic pleural effusion, abscess, hepatoma and insufficiency. Here, we report a case diaphragmatic hernia in patient following malignancy with cirrhosis.