A5070714596- Autism Spectrum Disorder Research
- Genetics and Neurodevelopmental Disorders
- Arsenic contamination and mitigation
- Heavy Metal Exposure and Toxicity
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Heavy metals in environment
- Retinoids in leukemia and cellular processes
- Retinal Development and Disorders
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Pregnancy and preeclampsia studies
- Trace Elements in Health
- Alzheimer's disease research and treatments
- Helicobacter pylori-related gastroenterology studies
- Studies on Chitinases and Chitosanases
- Clostridium difficile and Clostridium perfringens research
- Hedgehog Signaling Pathway Studies
- Selenium in Biological Systems
- Peptidase Inhibition and Analysis
- Optical Imaging and Spectroscopy Techniques
- Microscopic Colitis
- Non-Invasive Vital Sign Monitoring
- SARS-CoV-2 and COVID-19 Research
- Vitamin C and Antioxidants Research
Shenzhen University
2021-2025
Shinshu University
2016-2021
Northeast Normal University
2019
Abstract Genome editing is a powerful technique for studying gene functions. CRISPR/Cas9-mediated knock-in has recently been applied to various cells and organisms. Here, we successfully knocked in an EGFP coding sequence at the site immediately after first ATG codon of β-actin neurons brain by combined use CRISPR/Cas9 system utero electroporation technique, resulting expression EGFP-tagged protein cortical layer 2/3 pyramidal neurons. We detected fluorescence signals soma neurites These...
Arsenic (As) is a toxic element, and long-term exposure to As can cause neurotoxicity. The bioactive natural compound Dictyophora polysaccharide (DIP) from edible plants has been reported reduce the toxicity of As. In this study, poisoning was simulated by feeding As-containing feed, followed proteomic analysis after one month DIP treatment. showed that 145, 276, 97 proteins were differentially expressed between As-treated rats control (As/Ctrl group), DIP-treated + (DIP As/As As/Ctrl...
Calcium/calmodulin-dependent serine protein kinase (CASK) is a membrane-associated guanylate (MAGUK) that associated with neurodevelopmental disorders. CASK thought to have both pre- and postsynaptic functions, but the mechanism consequences of its functions in brain yet be elucidated, because homozygous CASK-knockout (CASK-KO) mice die before maturation. Taking advantage X-chromosome inactivation (XCI) mechanism, here we examined synaptic CASK-KO neurons acute slices heterozygous female...
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Genetic heterogeneity poses a challenge to research and clinical translation of autism spectrum disorder (ASD). In this study, we conducted plasma proteomic metabolomic study children with ASD without risk genes (de novo mutation) controls explore the impact genetic on search for biomarkers ASD. terms profiles, groups carrying those not de mutation tended cluster overlap, integrating them yielded differentially expressed proteins differential metabolites that effectively distinguished from...
Abstract Alzheimer’s disease (AD) is a multifactorial neurodegenerative that lacks convenient and accessible peripheral blood diagnostic markers effective drugs. Metabolic dysfunction one of AD risk factors, which leaded to alterations various metabolites in the body. Pathological changes brain can be reflected are expected explain mechanisms or candidate biomarkers. The aim this study was investigate targeted within mouse model, with purpose exploring mechanism potential Targeted...
Autism spectrum disorder (ASD) is a neurological and developmental characterized by social communication difficulties. Valproic acid (VPA) injection during pregnancy elicits autism-like behavior in the offspring, making it classic animal model of ASD. However, mechanisms involved have not yet been determined. In this study, we used iTRAQ (isobaric tags for relative absolute quantification) proteomics analysis cerebral cortex VPA rat (VPA group) controls (CON group). The results showed that...
Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought identify potential proteins placental tissue that may be involved pathogenesis ICP adverse fetal outcomes.The postpartum pregnant women with were chosen as case group (ICP group) (subdivided into mild (MICP severe (SICP...
Microcephaly with pontine and cerebellar hypoplasia (MICPCH) syndrome is a neurodevelopmental disorder caused by the deficiency of X-chromosomal gene CASK. However, molecular mechanisms which CASK causes in this remain elusive. In study, we used knockout (KO) mice as models for MICPCH investigated effect mutants. Female heterozygote KO replicate progressive observed syndrome. cultured granule (CG) cells show cell death that can be rescued co-infection lentivirus expressing wild-type Rescue...
Arsenic (As) is a widespread metalloid and human carcinogen found in the natural environment, multiple toxic effects have been shown to be associated with As exposure. can accumulated spleen, largest peripheral lymphatic organ, long-term exposure lead splenic injury. In this study, Sprague-Dawley (SD) rat model of As-poisoned was established, aiming explore molecular mechanism As-induced immune injury through combined analysis proteomics metabolomics rats' spleen. After feeding rats diet (50...