A5074417822- Health Sciences Research and Education
- Pasture and Agricultural Systems
- Molecular Biology Techniques and Applications
- Library Science and Administration
- Livestock and Poultry Management
- Library Science and Information Literacy
- Health Literacy and Information Accessibility
- Invertebrate Taxonomy and Ecology
- Web and Library Services
- Gene expression and cancer classification
- Library Collection Development and Digital Resources
- Botany, Ecology, and Taxonomy Studies
- Healthcare Systems and Technology
- Cancer Cells and Metastasis
- Social Media in Health Education
- American Environmental and Regional History
- Viral-associated cancers and disorders
- Academic Writing and Publishing
- Polyomavirus and related diseases
- Archaeology and Natural History
- Advanced Biosensing Techniques and Applications
- RNA modifications and cancer
- Telomeres, Telomerase, and Senescence
- Cytomegalovirus and herpesvirus research
- Mobile Health and mHealth Applications
University of Tennessee at Knoxville
2019-2024
University of Tennessee Medical Center
2019-2024
Atlantic Health System
2023
TH Köln - University of Applied Sciences
2018-2022
Center for Cancer Research
2005-2021
Platinum Group Coatings (United States)
2021
Academy of Vocal Arts
2019-2020
St. Leonards Hospital
2019-2020
National Cancer Institute
2001-2020
ORCID
2020
A small percentage of bladder cancers in the general population have been found to harbor DNA viruses. In contrast, up 25% tumors solid organ transplant recipients, who are at an increased risk developing cancer and overall poorer outcomes, BK polyomavirus (BKPyV). To better understand biology mechanisms carcinogenesis from potential oncoviruses, we performed whole genome transcriptome sequencing on specimens 43 patients. Nearly half this patient contained viral sequences. The most common...
Microarrays for the analysis of gene expression are three different types: short oligonucleotide (25–30 base), long (50–80 and cDNA (highly variable in length). The arrays have been mainstay to date, but platforms gaining popularity will probably replace arrays. As part a validation study arrays, we compared contrasted profiles from formats, testing RNA six cell lines against universal reference standard. had 6430 genes common. In general, correlation levels across was good when defined by...
Spot intensity serves as a proxy for gene expression in dual-label microarray experiments. Dye bias is defined an difference between samples labeled with different dyes attributable to the instead of samples. that not removed by array normalization can introduce into comparisons interest. But if consistent across same gene, it be corrected proper experimental design and analysis. If dye but samples, then removing becomes more problematic, perhaps indicating technical limitation ability...
Job postings for medical and health sciences librarians provide valuable data those seeking a better understanding of the evolving field librarianship. Our indicate decrease in number from 2022 to 2024, modest increase percentage advertising remote/hybrid work, an average minimum posted salary, majority focused on one or more public service components library services. Utilizing this provides complete picture profession transition.
We have investigated gene expression profiles of human ovarian carcinomas in vivo during Taxol(R) (paclitaxel) treatment and observed a difference expression. Nude mice bearing 1A9 or 1A9PTX22 xenografts were given 60 mg/kg paclitaxel. Therapeutic efficacy was achieved for 1A9, while did not respond. Tumor tissues harvested 4 24 h after evaluated by cDNA microarray against untreated tumors. Paclitaxel caused the modulation more genes than tumors, accordance to their therapeutic response....
The clinical course of breast cancer varies from one patient to another. Currently, the choice therapy relies on parameters and histological molecular tumor features. Alas, these markers are informative in only a subset patients. Therefore, additional predictors disease outcome would be valuable for treatment stratification. Extensive studies showed that degree variation nuclear DNA content, i.e., aneuploidy, determines prognosis. Our aim was further elucidate basis aneuploidy. We analyzed...
Abstract Purpose To compare the deoxyribonucleic acid (DNA) methylation signature of neuroendocrine tumors (NETs) by primary tumor site and inherited predisposition syndromes von Hippel–Lindau disease (VHL) multiple endocrine neoplasia type 1 (MEN1). Methods Genome-wide DNA (835 424 CpGs) 96 NET samples. Principal components analysis (PCA) unsupervised hierarchical clustering analyses were used to determine methylome signatures. Results Hypomethylated CpGs significantly more common in...
Abstract Background In human breast cancer normal mammary cells typically develop into hyperplasia, ductal carcinoma in situ , invasive cancer, and metastasis. The changes gene expression associated with this stepwise progression are unclear. Mice transgenic for mouse tumor virus (MMTV)- Wnt-1 exhibit discrete steps of tumorigenesis, including carcinoma, distant These mice might therefore be useful models discovering during development. Results We used cDNA microarrays to determine the...
Understanding the language encrypted in gene regulatory regions of human genome is a challenging goal for genomic era. Although customary extrapolations from steady-state mRNA levels have been effective, deciphering these codes will require additional empirical data sets that more closely reflect dynamic progression molecular events responsible inducible transcription. We describe an approach using chromatin immunoprecipitation to profile kinetic occupancy transcriptional coactivator and...
NF-κB is an inducible transcription factor that controls kinetically complex patterns of gene expression. Several studies reveal multiple pathways linking to the promotion and progression various cancers. Despite extensive interest characterization, many controlled genes still remain be identified. We used chromatin immunoprecipitation combined with microarray technology (ChIP/Chip) investigate dynamic interaction promoter regions 100 known expressed in mitogen-induced T-cells. Six...
Cancer stem-like cells are hypothesized to be the major tumor-initiating cell population of human cutaneous squamous carcinoma (cSCC), but landscape molecular alterations underpinning their signaling and cellular phenotypes as drug targets remains undefined. In this study, we developed an experimental pipeline isolate a highly enriched CD133+CD31-CD45-CD61-CD24- (CD133+) from primary cSCC specimens by flow cytometry. The CD133+ show enhanced phenotypes, which were verified spheroid colony...
This study compares health science librarian job postings in the MEDLIB-L listserv from 2018-2019 and 2021-2022 to assess whether there was an increase remote or hybrid schedule arrangements listed advertisements after pandemic's onset. Results indicated a notable advertising remote/hybrid work rising 1.2% of listings 16% 2021-2022. Data 2022 survey library directors, however, that approximately 70% respondents expressed confidence continuance work. Additionally, very limited sample size,...
We have demonstrated in vitro transcription (IVT) of cDNA sequences from purified Jurkat T-cell mRNA immobilized on microfluidic packed beds down to single-cell quantities. The microfluidically amplified antisense-RNA (aRNA) was nearly identical length and quantity compared with benchtop reactions using the same starting sample Microarrays were used characterize number population genes each sample, allowing comparison processes. For both assays, we measured expression approximately 4000 9000...
"Approaches to Liaison Librarianship: Innovations in Organization and Engagement." Medical Reference Services Quarterly, 41(3), pp. 328–329
The ability to be flexible and adapt quickly changing circumstances is a crucial skill for librarians develop in world increasingly characterized by rapid change. It can take crisis learn how effective have become developing the needed adaptive behaviors, including willingness change workstyles, experiment with new technologies readily move on from failed experiments. In this paper, Preston Medical Library at University of Tennessee Graduate School Medicine, prompted COVID-19, present their...