A5089011260- Endometrial and Cervical Cancer Treatments
- Kruppel-like factors research
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Cancer Cells and Metastasis
- Genetic Syndromes and Imprinting
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Extracellular vesicles in disease
- Heme Oxygenase-1 and Carbon Monoxide
- Angiogenesis and VEGF in Cancer
- Genetic Mapping and Diversity in Plants and Animals
- Endometriosis Research and Treatment
- Genetic factors in colorectal cancer
- Ubiquitin and proteasome pathways
- Eicosanoids and Hypertension Pharmacology
- Peroxisome Proliferator-Activated Receptors
- Sarcoma Diagnosis and Treatment
- HER2/EGFR in Cancer Research
- Estrogen and related hormone effects
- Cancer-related Molecular Pathways
- Prostate Cancer Treatment and Research
Vall d'Hebron Institut de Recerca
2016-2024
Universitat Autònoma de Barcelona
2016-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2009-2024
Centro de Investigación Biomédica en Red de Cáncer
2017-2024
Experimental Medicine and Biology Institute
2014-2024
Universitat de Vic - Universitat Central de Catalunya
2024
Biomedical Research Institute of Lleida
2018-2019
Universitat de Lleida
2019
Hospital Universitari Arnau de Vilanova
2018
University of Buenos Aires
2009-2014
Abstract The BRCA1 gene product plays numerous roles in regulating genome integrity. Its role assembling supermolecular complexes response to DNA damage has been extensively studied; however, much less is understood about its as a transcriptional coregulator. Loss or mutation associated with hereditary breast and ovarian cancers, whereas altered expression occurs frequently sporadic forms of cancer, suggesting that the control transcription might be important tumorigenesis. Here, we provide...
Abstract Endometrial cancer is the most common gynaecological in western countries, being subtype of endometrioid tumours. Most patients are diagnosed at an early stage and present excellent prognosis. However, a number those continue to suffer recurrence, without means identification by risk classification systems. Thus, finding reliable marker predict recurrence becomes important unmet clinical issue. ALCAM cell–cell adhesion molecule member immunoglobulin superfamily that has been...
Abstract Lung cancer is the leading cause of cancer-related deaths among men and women in world, accounting for 25% mortality. Early diagnosis an unmet clinical issue. In this work, we focused to develop a novel approach identify highly sensitive specific biomarkers by investigating use extracellular vesicles (EVs) isolated from pleural lavage, proximal fluid lung patients, as source potential biomarkers. We EVs ultracentrifuge method 25 control fluids 21 lavages patients. Analysis...
Abstract The analysis of mismatch repair proteins in solid tissue is the standard care (SoC) for microsatellite instability (MSI) characterization endometrial cancer (EC). Uterine aspirates (UAs) or circulating‐DNA (cfDNA) samples capture intratumor heterogeneity and provide a more comprehensive dynamic molecular diagnosis. Thus, MSI by droplet‐digital PCR (ddPCR) UAs cfDNA can reliable tool to characterize follow‐up disease. UAs, paraffin‐embedded tumor (FFPE) longitudinal plasma from...
Abstract Endometrial cancer (EC) remains the most common malignancy of genital tract among women in developed countries. Although much research has been performed at genomic, transcriptomic and proteomic level, there is still a significant gap metabolomic studies EC. In order to gain insights into altered metabolic pathways onset progression EC carcinogenesis, we used high resolution mass spectrometry characterize lipidomic profile 39 human 17 healthy endometrial tissue samples. Several...
Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development a minimally-invasive diagnosis, based on identification sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk.In this study, we aim to develop novel approach identify highly and specific by investigating use extracellular vesicles (EVs) isolated from peritoneal lavage as...
Endometrial cancer (EC) is the sixth most common in women worldwide and responsible for more than 89,000 deaths every year. Mortality associated with presence of poor prognostic factors at diagnosis, i.e., diagnosis an advanced stage, a high grade and/or aggressive histology. Development novel approaches that would permit us to improve clinical management EC patients unmet need. In this study, we investigate approach identify highly sensitive specific biomarkers using extracellular vesicles...
The incidence and mortality of endometrial cancer (EC) have risen in recent years, hence more precise management is needed. Therefore, we combined different types liquid biopsies to better characterize the genetic landscape EC a non-invasive dynamic manner. Uterine aspirates (UAs) from 60 patients with were obtained during surgery analyzed by next-generation sequencing (NGS). Blood samples, collected at surgery, used for cell-free DNA (cfDNA) circulating tumor cell (CTC) analyses. Finally,...
Pre-operative immunohistochemical (IHC) biomarkers are not incorporated in endometrial cancer (EC) risk classification. We aim to investigate the added prognostic relevance of IHC ESMO-ESGO-ESTRO classification and lymph node (LN) status EC.Retrospective multicenter study within European Network for Individualized Treatment Endometrial Cancer (ENITEC), analyzing pre-operative expression p53, L1 cell-adhesion molecule (L1CAM), estrogen receptor (ER) progesterone (PR), relate groups, LN...
Analyzing different tumor regions by next generation sequencing allows the assessment of intratumor genetic heterogeneity (ITGH), a phenomenon that has been studied widely in some types but less well explored endometrial carcinoma (EC). In this study, we sought to characterize spatial and temporal 9 ECs using whole-exome sequencing, performing targeted validation 42 primary 30 metastatic samples analyzed. addition, copy number alterations serous carcinomas were assessed comparative genomic...
Human PTEFb is a protein kinase composed by CDK9 and Cyclin T that controls the elongation phase of RNA Pol II. This complex also affects activation differentiation program lymphoid cells. In this study we found several head neck tumor cell lines overexpress PTEFb. We established T1 able to induce transformation in vitro, as determined foci colony formation assays. Nu/nu mice s.c. injected with stable transfected cells (NIH 3T3 T1) developed tumors faster than animals control b-gal). NIH...
Abstract Purpose: Clinical and epidemiologic data suggest that obesity is associated with more aggressive forms of prostate cancer, poor prognosis, increased mortality. C-terminal–binding protein 1 (CtBP1) a transcription repressor tumor suppressor genes activated by NADH binding. High calorie intake decreases intracellular NAD+/NADH ratio. The aim this work was to assess the effect high-fat diet (HFD) CtBP1 expression modulation over xenograft growth. Experimental Design: We developed...
PTEN is one of the most frequently mutated genes in human cancers. The frequency alterations particularly high endometrial carcinomas. Loss leads to dysregulation cell division, and promotes accumulation cycle complexes such as cyclin D1-CDK4/6, which an important feature tumour phenotype. Cell proteins have been presented key targets treatment pathogenesis cancer, several CDK inhibitors developed a strategy generate new anticancer drugs. Palbociclib (PD-332991) specifically inhibits CDK4/6,...
NF-κB is an inducible transcription factor that controls kinetically complex patterns of gene expression. Several studies reveal multiple pathways linking to the promotion and progression various cancers. Despite extensive interest characterization, many controlled genes still remain be identified. We used chromatin immunoprecipitation combined with microarray technology (ChIP/Chip) investigate dynamic interaction promoter regions 100 known expressed in mitogen-induced T-cells. Six...
Abstract BRCA1 plays numerous roles in the regulation of genome integrity and chemoresistance. Although interaction with key proteins involved DNA repair is well known, its role as a coregulator transcriptional response to damage remains poorly understood. In this study, we show that central genotoxic stress prostate cancer. expression mediates apoptosis, cell-cycle arrest, decreased viability doxorubicin treatment. Xenograft studies using human carcinoma PC3 cells depletion results...
Endometrial cancer (EC) patients with metastatic/recurrent disease have limited treatment options and poor survival outcomes. Recently, we discovered the FGFR2c splice isoform is associated prognosis in EC patients. Here report establishment of 16 patient-derived xenografts (PDX)-derived organoids (PDXOs) or without expression. In vitro 5 PDXOs BGJ398 showed significant cell death 3 models PDXs high/moderate expression tumour growth inhibition (TGI) following 21-day FGFR inhibitors (BGJ398...
// Paola De Luca 1 , Guillermo N. Dalton Georgina D. Scalise Cristian P. Moiola Juliana Porretti Cintia Massillo Edith Kordon 2 Kevin Gardner 3 Florencia Zalazar Carolina Flumian 4 Laura Todaro Elba S. Vazquez 5 Roberto Meiss 6 Adriana Siervi Laboratorio de Oncología Molecular y Nuevos Blancos Terapéuticos, Instituto Biología Medicina Experimental (IBYME), CONICET, Buenos Aires, Argentina Departamento Fisiología, Celular, Facultad Ciencias Exactas Naturales (FCEN), Universidad Aires (UBA),...
Objective: Endometrial cancer (EC) is the second most common gynecological worldwide. Myometrial invasion (MI) a key event in EC dissemination. This study aimed to evaluate FXYD5/dysadherin (FXYD5/Dys) expression tissue and uterine aspirate (UA) biopsies assess molecular/functional changes associated with its cellular models. Methods: FXYD5/Dys messenger RNA (mRNA) levels were determined UA biopsies. was evaluated RNAseq data from The Cancer Genome Atlas (TCGA) GENEVESTIGATOR tools. impact...
Endometrial cancer (EC) is the second most frequent gynecological worldwide. Although improvements in EC classification have enabled an accurate establishment of disease prognosis, women with a high-risk or recurrent face dramatic situation due to limited further treatment options. Therefore, new strategies that closely mimic are required maximize drug development success. Patient-derived xenografts (PDXs) widely recognized as physiologically relevant preclinical model. Hence, we propose...