A5001323854- Mitochondrial Function and Pathology
- Biological and pharmacological studies of plants
- Retinal Development and Disorders
- melanin and skin pigmentation
- Plant and animal studies
- RNA modifications and cancer
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Protein Tyrosine Phosphatases
- Phytochemistry and Biological Activities
- Virus-based gene therapy research
- Ginseng Biological Effects and Applications
- MicroRNA in disease regulation
- Biochemical Analysis and Sensing Techniques
- Retinal Diseases and Treatments
- Bladder and Urothelial Cancer Treatments
- RNA Interference and Gene Delivery
- Olfactory and Sensory Function Studies
- Cancer Research and Treatments
- Melanoma and MAPK Pathways
- PARP inhibition in cancer therapy
- CRISPR and Genetic Engineering
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Glaucoma and retinal disorders
Ocean University of China
2012-2025
Hangzhou Normal University
2024
Nanjing Medical University
2023-2024
Jiangsu Province Hospital
2024
Taizhou People's Hospital
2023-2024
Nantong University
2017-2024
Institute of Botany
2024
Chinese Academy of Sciences
2018-2024
Shandong Agricultural University
2023-2024
ZheJiang Institute For Food and Drug Control
2024
Many G protein-coupled receptors (e.g. that of angiotensin II) activate phospholipase Cβ, initially increasing intracellular calcium and activating protein kinase C. In the WB GN4 rat liver epithelial cell lines, agonist-induced signals also stimulate tyrosine phosphorylation subsequently increase activity c-Jun N-terminal (JNK). We have now purified major calcium-dependent (CADTK), by peptide nucleic acid sequencing identified it as a homologue human PYK2. CADTK/PYK2 is most closely related...
Low salinity is one of the main factors limiting distribution and survival marine species. As a euryhaline species, Pacific oyster Crassostrea gigas considered to be tolerant relative low salinity. The genes that regulate C. responses osmotic stress were monitored using next-generation sequencing whole transcriptome with samples taken from gills. By RNAseq technology, transcript catalogs up- down-regulated generated oysters exposed optimal seawater.Through Illumina sequencing, we reported...
To introduce DNA into mitochondria efficiently, we fused adenoassociated virus capsid VP2 with a mitochondrial targeting sequence to carry the gene encoding human NADH ubiquinone oxidoreductase subunit 4 (ND4). Expression of WT ND4 in cells G11778A mutation led restoration defective ATP synthesis. Furthermore, injection rodent eye, levels reached 80% its mouse homolog. The construct expressed most inner retinal neurons, and it also suppressed visual loss optic atrophy induced by mutant...
Melanocytes sustain a lifelong proliferative potential, but stem cell reservoir in glabrous skin has not yet been found. Here, we show that multipotent dermal cells isolated from human foreskins lacking hair follicles are able to home the epidermis differentiate into melanocytes. These cells, grown as three-dimensional spheres, displayed capacity for self-renewal and expressed NGFRp75, nestin OCT4, melanocyte markers. In addition, derived single-cell clones were multiple lineages including...
Gentiana rigescens is an economically important medicinal plant in the subtropical zone Asia. However, it unclear how its distribution responds to climate change future. This study aims simulate effect of on G. by using maximum entropy (MaxEnt) model and evaluate potential impact habitat suitability quality materials vis multiple chemical information. In study, we investigated suitable fluctuation ranges under different future scenarios at first. Subsequently, geographical variation...
Macrophage polarization determines the production of cytokines that fuel initiation and evolution rheumatoid arthritis (RA). Thus, modulation macrophage might represent a potential therapeutic strategy for RA. However, coordinated macrophages in synovium synovial fluid has not been achieved thus far. Herein, we develop biomimetic ApoA-I mimetic peptide-modified neutrophil membrane-wrapped F127 polymer (R4F-NM@F127) targeted drug delivery during RA treatment. Due to high expression adhesion...
The regulation of the informational flow from mitochondria to nucleus (mitonuclear communication) is not fully characterized in heart. We have determined that mitochondrial ribosomal protein S5 (MRPS5/uS5m) can regulate cardiac function and key pathways coordinate this process during stress. demonstrate loss Mrps5 developing heart leads defects embryonic lethality while postnatal induces hypertrophy failure. structure disrupted mutant cardiomyocytes, impairing translation OXPHOS. identify...
In GN4 rat liver epithelial cells, angiotensin II (Ang II) and other agonists which activate phospholipase C stimulate tyrosine kinase activity in a calcium-dependent, protein (PKC)-independent manner.Since Ang also produces proliferative response these we investigated downstream signaling elements traditionally linked to growth control by kinases.First, II, like epidermal factor (EGF), stimulated AP-1 binding PKC-independent manner.Because increases can reflect induction of c-Jun c-Fos,...
The ripening of avocado fruit is associated with the expression a number mRNAs concomitant overt changes in texture and flavor. Two overlapping cDNAs for mRNA that accumulates during were identified. Sequence analysis these two revealed polypeptide 471 amino acids characteristics typical P-450: an N-terminal hydrophobic membrane anchor, conserved heme-binding domain C-terminal region, patches similarity to various P-450 family members. Further evidence this represents cytochrome oxidase...
Abstract Mutations in the BRAF serine/threonine kinase gene are frequently found cutaneous melanomas. Activation of hypoxia inducible factor-1α (HIF-1α) response to both hypoxic stress and oncogenic signals has important implications cancer development progression. Here, we report that mutant BRAFV600E increases HIF-1α expression melanoma cells. Our microarray profiling data 35 melanocyte cell lines showed was significantly increased melanomas harboring mutation. Stable suppression or...
Cytochrome c (Cyt c) is part of the mitochondrial electron transport chain (ETC), accepting electrons from bc(1) complex and transferring them to cytochrome oxidase (CcO). The ETC generates membrane potential, which used by ATP synthase produce ATP. In addition, release Cyt mitochondria often commits a cell undergo apoptosis. Considering its central role in life (respiration) death (apoptosis) decisions one would expect tight regulation function. Reversible phosphorylation main cellular...
We developed a novel strategy for treatment of Leber hereditary optic neuropathy (LHON) caused by mutation in the nicotinamide adenine dinucleotide dehydrogenase subunit IV (ND4) mitochondrial gene.OBJECTIVE To demonstrate safety and effects gene therapy vector to be used proposed clinical trial.DESIGN AND SETTING In series laboratory experiments, we modified ND4 complex I nuclear genetic code import into mitochondria.The protein was targeted organelle agency targeting sequence (allotopic...
Taxonomy and phylogeny of subclass Heterodonta including Tellinoidea are long-debated issues a complete agreement has not been reached yet. Mitochondrial (mt) genomes have proved to be powerful tool in resolving phylogenetic relationship. However, date, only ten mitochondrial Heterodonta, which is by far the most diverse major group Bivalvia, determined. In this paper, we newly sequenced mt six species belonging order resolve some problematical relationships among subclass.The vary size from...
Long-chain fatty acids (LCFAs) are involved in regulating multiple physiological processes as signalling molecules. Gas chromatography-mass spectrometry (GC-MS) is widely used to quantify LCFAs. However, current quantitative methods for LCFAs using GC-MS have demonstrated complicated issues. Psoriasis a chronic inflammatory skin disease, and its pathogenesis may be related the overproduction of interleukin-17A (IL-17A). Clinical efficacy anti-IL-17A monoclonal antibody (mAb) treatment...
Purpose.: To demonstrate safety and efficacy of allotopic human ND4 for treatment a Leber's hereditary optic neuropathy (LHON) mouse model harboring the G11778A mitochondrial mutation. Methods.: We induced LHON in mice by intravitreal injection mutant (G11778A) DNA, responsible most cases LHON, that was directed to mitochondria using an AAV2 vector which we appended targeting sequence VP2 capsid. then attempted rescue visual loss our test article (ScAAV2-P1ND4v2) containing synthetic nuclear...