Sustained release of bioactive BMP2 (bone morphogenetic protein-2) is important for bone regeneration, while the intrinsic short half-life at protein level cannot meet clinical need. In this study, we aimed to design Bmp2 mRNA-enriched engineered exosomes, which were then loaded into specific hydrogel achieve sustained more efficient and safe regeneration.Bmp2 mRNA was enriched exosomes by selective inhibition translation in donor cells, NoBody (non-annotated P-body dissociating polypeptide,...

Periodontal regeneration involves the restoration of at least three unique tissues: cementum, periodontal ligament tissue (PDL) and alveolar bone tissue. Here, we first isolated human PDL stem cells (PDLSCs) jaw mesenchymal (JBMSCs). These were then induced to form cell sheets using an ascorbic acid-rich approach, sheet properties, including morphology, thickness gene expression profile, compared. Platelet-rich fibrin (PRF) derived from venous blood was fabricated into bioabsorbable...

Abstract: Dental implants have been widely used for the replacement of missing teeth in clinic, but further improvements are needed to meet clinical demands faster and tighter osseointegration. In this study, we fabricated safe biocompatible chitosan (CS)/hyaluronic acid (HA) nanoparticles deliver microRNA-21 (miR-21) thereby accelerate osteogenesis human bone marrow mesenchymal stem cells (hBMMSCs). The CS/HA/miR-21 were cross-linked with 0.2% gel solution onto microarc oxidation...

Abstract: Cell sheet engineering has emerged as a novel approach to effectively deliver seeding cells for tissue regeneration, and developing human bone marrow mesenchymal stem cell (hBMMSC) sheets with high osteogenic ability is constant requirement from clinics faster higher-quality formation. In this work, we fabricated biocompatible safe chitosan (CS)/hyaluronic acid (HA) nanoparticles (NPs) microRNA-21 (miR-21), which been proved accelerate osteogenesis in hBMMSCs; then, the...

Objective: To accelerate wound healing through promoting vascularization by using reactive oxygen species (ROS)-responsive nanoparticles loaded with stromal cell-derived factor-1α (SDF-1α). Methods: The ROS-reactive nanomaterial poly-(1,4-phenyleneacetone dimethylene thioketal) was synthesized, and its physical chemical properties were characterized. ROS-responsive containing SDF-1α prepared a multiple emulsion solvent evaporation method. loading capacity, stability, activity of the...

Irradiated bone has a greater risk of implant failure than nonirradiated bone. The purpose this study was to investigate the influence cell sheets composed co-cultured marrow mesenchymal stromal cells (BMSCs) and endothelial progenitor (EPCs) on osseointegration in irradiated Cell (EPCs, BMSCs or EPCs BMSCs) were wrapped around titanium implants make sheet-implant complexes. group showed highest osteogenic differentiation potential vitro, as indicated by extracellular matrix mineralization...

The complete assignment of the 1 H and 13 C nmr signals for Qinghaosu has been made. Treatment with methoxide ion at 80 °C results in bright luminescence when carried out presence fluorescers. Several products are formed methanol is present, but toluene only one product methanol-free used. Its structure tentatively assigned as 1-(1-methoxycarbonylethyl)-2,6-dimethyl-2-hydroxybicyclo[3.3.1]nonan-9-one.

IκBβis an inhibitor of nuclear factor kappa B(NF-κB) and participates in the cardiac response to sepsis. However, role hypo-phosphorylated form IκBβ at Ser313, which can be detected during sepsis, is unknown. Here, we examined effects with a mutation Ser313→Ala313 on damage induced by Transgenic (Tg) mice were generated overexpress IκBβ, Ser-313 replaced alanine ubiquitously, order mimic IκBβ. Survival analysis showed that Tg exhibited decreased inflammatory cytokine levels rates mortality...

Forming a compact biological seal between the gingiva and implant interface around percutaneous parts of an is one key issues in preventing peri-implantitis.In this study, since microRNA-21 (miR-21) has been approved to promote fibroblast proliferation collagen formation skin fibrosis, we prepared miR-21-loaded chitosan (CS)/tripolyphosphate (TPP)/hyaluronic acid (HA) nanoparticles (CTH NPs) cross-linked them smooth Ti surfaces with 0.2% gel solution for reverse transfection, after which...

Outer membrane (OM) β-barrel proteins play important roles in importing nutrients, exporting wastes and conducting signals Gram-negative bacteria, mitochondria chloroplasts. The outer (OMPs) are inserted assembled into the OM by OMP85 family proteins. In Escherichia coli, assembly machinery (BAM) contains four lipoproteins such as BamB, BamC, BamD BamE, one OMP BamA, forming a 'top hat'-like structure. Structural functional studies of E. coli BAM have revealed that rotation periplasmic ring...

Total auricular reconstruction is still a challenge, and autologous cartilage transplant the main therapy so far. Tissue engineering provides promising method for reconstruction. However, although degradable framework demonstrated excellent initial cosmetic details, it difficult to maintain contour over time metabolites tended be harmful human body. In this study, biocompatible safe nondegradable elastic polyurethane was used make porous scaffold in specific details by rapid prototyping...

Background: The fat repositioning technique has been widely used for the treatment of tear trough deformity, and there is a strong belief that excess herniation prerequisite procedure. Objective: purpose this study was to evaluate its effect in patients with minimal or no herniation. Methods: A total 232 underwent procedure met inclusion criteria. Of them, 198 were primary cases, 34 had history removal blepharoplasty. amount infraorbital evaluated preoperatively by palpation. Release...

Lipopolysaccharide (LPS) is essential for most gram-negative bacteria and plays an important role in serum resistance, pathogenesis, drug protection from harsh environments. The outer core oligosaccharide of LPS involved bacterial recognition invasion host cells. D-galactosyltransferase WaaB responsible the addition D-galactose to LPS, which Salmonella typhimurium invasion. Here we report first crystal structures complex with UDP resolutions 1.8 1.9 Å, respectively. Mutagenesis enzyme...

The cloning, expression and purification of the glutathione (sulfur) import system ATP-binding protein (gsiA) was carried out. coding sequence Escherichia coli gsiA, which encodes a importer, amplified by PCR, then inserted into prokaryotic vector pWaldo-GFPe harboring green fluorescent (GFP) reporter gene. resulting recombinant plasmid pWaldo-GFP-GsiA transformed various E. strains, conditions were optimized. effect five strains on production gsiA evaluated. BL21 (DE3) found to be most...

Objectives. To purify and characterize the glutathione binding protein GsiB of importer (GSI) in Escherichia coli (E. coli) . Results. The coding sequence was cloned from E. MG1655 expressed BL21(DE3). purified to homogeneity using Ni-affinity gel filtration chromatography. SDS-PAGE showed a single band molecular mass 56 kDa, while native two bands around kDa 110 kDa. Gene knockout that essential for GSI mediated import. Interactions GsiA, B, C, D were determined bacterial two-hybrid method....