A5063515197- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Nutrition and Health in Aging
- Exercise and Physiological Responses
- Diet and metabolism studies
- Sports Performance and Training
- Respiratory Support and Mechanisms
- Bariatric Surgery and Outcomes
- Metabolism, Diabetes, and Cancer
- Sports injuries and prevention
- Body Composition Measurement Techniques
- Neuroscience of respiration and sleep
- Mitochondrial Function and Pathology
- Neonatal Respiratory Health Research
- Genetics and Physical Performance
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Chemotherapy-induced cardiotoxicity and mitigation
- Thermoregulation and physiological responses
- Cardiovascular Effects of Exercise
- Cardiovascular and exercise physiology
- Spinal Cord Injury Research
- Children's Physical and Motor Development
- Tryptophan and brain disorders
- Neurogenetic and Muscular Disorders Research
AdventHealth Orlando
2019-2024
Translational Research Institute for Metabolism and Diabetes
2018-2024
Pepsi (United States)
2024
Gatorade Sports Science Institute
2024
East Carolina University
2013-2020
University of Florida
2017-2020
Ball State University
2011-2016
Piedmont International University
2016
To examine potential age-specific adaptations in skeletal muscle size and myofiber contractile physiology response to aerobic exercise, seven young (YM; 20 ± 1 yr) six older men (OM; 74 3 performed 12 wk of cycle ergometer training. Muscle biopsies were obtained from the vastus lateralis determine properties isolated slow [myosin heavy chain (MHC) I] fast (MHC IIa) myofibers, MHC composition, protein concentration. Aerobic capacity was higher ( P < 0.05) after training both YM (16 2%) OM...
Exercise-induced perturbation of skeletal muscle metabolites is a probable mediator long-term health benefits in older adults. Although specific have been identified to be impacted by age, physical activity and exercise, the depth coverage metabolome still limited. Here, we investigated resting exercise-induced metabolite distribution from well-phenotyped adults who were active or sedentary, group young Percutaneous biopsies vastus lateralis obtained before, immediately after 3 h following...
The loss of skeletal muscle mass and function with age (sarcopenia) is a critical healthcare challenge for older adults. 31-phosphorus magnetic resonance spectroscopy (31 P-MRS) powerful tool used to evaluate phosphorus metabolite levels in muscle. Here, we sought determine which metabolites were linked reduced This investigation was conducted across two separate studies. Resting examined by 31 P-MRS. In the first study, fifty-five adults obesity enrolled found that resting phosphocreatine...
Muscle transcript signatures associated with oxidative capacity and immune cells underlie important phenotypic clinical characteristics of older adults who are endurance trained or sedentary. Despite divergent phenotypes, the temporal transcriptional in response to an acute bout exercise were largely similar among groups. These data provide new insight into programs aging muscle beneficial effects promote healthy adults.
Mechanical ventilation (MV) is a life-saving intervention for many critically ill patients. Unfortunately, prolonged MV results in rapid diaphragmatic atrophy and contractile dysfunction, collectively termed ventilator-induced diaphragm dysfunction (VIDD). Recent evidence reveals that endurance exercise training, performed prior to MV, protects the against VIDD. While mechanism(s) responsible this exercise-induced protection VIDD remain unknown, increased antioxidant expression may be...
Exercise mediates tissue metabolic function through direct and indirect adaptations to acylcarnitine (AC) metabolism, but the exact mechanisms are unclear. We found that circulating medium-chain acylcarnitines (C12-C16) lower in active/endurance trained human subjects compared sedentary controls, this is correlated with elevated cardiorespiratory fitness reduced adiposity. In mice, exercise serum AC increased liver AC, was accompanied by a marked increase expression of genes involved hepatic...
The purpose of this investigation was to examine the influence short-term intense endurance training on cycling performance, along with acute and chronic signaling responses skeletal muscle stress stability markers. Ten recreationally active subjects (25 ± 2 yr, 79 3 kg, 47 ml·kg −1 ·min ) were studied before after a 12-day protocol effects (70–100% V̇o 2max exercise resting exercise-induced regulation molecular factors related cellular protein stability. Skeletal biopsies taken at rest h...
Oxidative damage to the diaphragm as a result of cervical spinal cord injury (SCI) promotes muscle atrophy and weakness. Respiratory insufficiency is leading cause morbidity mortality in patients, emphasizing need for strategies maintain function. Hyperbaric oxygen (HBO) increases amount dissolved into blood, elevating delivery skeletal reactive species (ROS) generation. It proposed that enhanced ROS production due HBO treatment stimulates adaptations oxidative capacity, resulting overall...
In insulin-sensitive skeletal muscle, the expression of constitutively active Ca2+/calmodulin-dependent protein kinase α (caCaMKKα) stimulates glucose uptake independent insulin signaling (i.e., Akt and Akt-dependent TBC1D1/TBC1D4 phosphorylation). Our objectives were to determine whether caCaMKKα could stimulate additively with in basal state insulin-resistant if so, effects associated altered phosphorylation. Mice fed a control or high-fat diet (60% kcal) for 12 weeks induce resistance....
The purpose of this study was to determine whether intramyocellular glucose partitioning altered in primary human myotubes derived from severely obese women with type 2 diabetes. Human skeletal muscle cells were obtained lean nondiabetic and Caucasian females diabetes [body mass index (BMI): 23.6 ± 2.6 vs. 48.8 1.9 kg/m , fasting glucose: 86.9 1.6 135.6 12.0 mg/dL, n = 9/group]. 1-[ 14 C]-Glucose metabolism (glycogen synthesis, oxidation, nonoxidized glycolysis) 1- 2-[ C]-pyruvate oxidation...
Doxorubicin (DOX) is a highly effective chemotherapeutic agent used in the treatment of broad spectrum cancers. However, clinical use DOX limited by irreversible and dose-dependent hepatotoxicity. The liver primary organ responsible for clearance antineoplastic agents, evidence indicates that hepatotoxicity occurs as result impaired mitochondrial efficiency during metabolism. In this regard, exercise training sufficient to improve function protect against DOX-induced cytotoxicity. Therefore,...
Mechanical ventilation (MV) is a clinical tool providing adequate alveolar in patients that require respiratory support. Although life-saving intervention for critically ill patients, prolonged MV results the rapid development of inspiratory muscle weakness due to both diaphragmatic atrophy and contractile dysfunction; collectively known as "ventilator-induced diaphragm dysfunction" (VIDD). VIDD severe problem because risk factor difficulties weaning from MV. Currently, no standard treatment...
Evidence suggests that myofibers from endurance trained skeletal muscle display unique contractile parameters. However, the underlying mechanisms remain unclear. To further elucidate influence of training on myofiber function, we examined factors may impact myofilament interactions (i. e., water content, concentration specific protein fractions, actin and myosin content) or directly modulate heavy chain (MHC) function light (MLC) composition) in biopsy samples highly-trained competitive...
Contractile activity (e.g., exercise) evokes numerous metabolic adaptations in human skeletal muscle, including enhanced insulin action and substrate oxidation. However, there is intersubject variation the physiological responses to exercise, which may be linked with factors such as degree of obesity. Roux-en-Y gastric bypass (RYGB) surgery reduces body mass severely obese (body index ≥ 40 kg/m2) individuals; however, it uncertain whether RYGB can potentiate contractile this potentially...
We previously reported an increase in skeletal muscle protein synthesis during fasted and fed recovery from nonexhaustive aerobic exercise (Harber et al., 2010). The current study examined intracellular signaling the same subjects to further investigate mechanisms of metabolism with without feeding following exercise. Eight males (VO 2peak : 52 ± 2 ml −1 .kg .min ) performed 60-min cycle ergometry at 72 1% VO on two occasions a counter-balanced design. Exercise trials differed only...
ABSTRACT Background Skeletal muscle from lean and obese subjects elicits differential adaptations in response to exercise/muscle contractions. In order determine whether obesity alters the mitochondrial dynamics contractions any of these distinct are linked alterations insulin sensitivity, we compared effects electrical pulse stimulation (EPS) on network structure regulatory proteins myotubes humans with severe evaluated correlations between signaling. Methods Myotubes human skeletal cells...
Mechanical ventilation (MV) is a life‐saving intervention for many critically ill patients. Unfortunately, prolonged MV results in the rapid development of inspiratory muscle weakness due to diaphragmatic atrophy and contractile dysfunction (termed ventilator‐induced diaphragm (VIDD)). Although VIDD major risk factor problems weaning patients from MV, standard therapy prevent does not exist. However, emerging evidence suggests that pharmacological blockade angiotensin II type 1 receptors...
Abstract The purpose of this study was to determine the effect hydration status on change in sweat sodium (Na + ), chloride (Cl − and potassium (K ) concentrations during exercise‐heat stress. Fifteen subjects (Six female, nine male; 29 ± 9 y; 71 14 kg) completed 90 min cycling (81% HR max heat (~33°C, 42% rh) with fluid replacement maintain euhydration (EUH) or without dehydrate 2.4 0.4% body mass loss (DEH). Sweat collected from forehead (FH), right scapula (SCAP), left (LVFA) (RVFA)...