A5100674376- Gastrointestinal motility and disorders
- Neuropeptides and Animal Physiology
- Ion Channels and Receptors
- Diet and metabolism studies
- Helicobacter pylori-related gastroenterology studies
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Adenosine and Purinergic Signaling
- Gastroesophageal reflux and treatments
- Pathogenesis and Treatment of Hiccups
- Cancer Cells and Metastasis
- Stress Responses and Cortisol
- RNA modifications and cancer
- Asthma and respiratory diseases
- Biochemical Analysis and Sensing Techniques
- Cellular Mechanics and Interactions
- Nanoplatforms for cancer theranostics
- Block Copolymer Self-Assembly
- Hepatocellular Carcinoma Treatment and Prognosis
- Congenital gastrointestinal and neural anomalies
- Advanced Nanomaterials in Catalysis
- Osteoarthritis Treatment and Mechanisms
- Infant Health and Development
- Pharmacological Effects of Natural Compounds
- Spaceflight effects on biology
Soochow University
2020-2024
Indiana University School of Medicine
2020-2024
Nanjing Drum Tower Hospital
2024
Southeast University
2024
The First Affiliated Hospital, Sun Yat-sen University
2020-2024
Sun Yat-sen University
2020-2024
Taiyuan University of Technology
2022-2024
University of Indianapolis
2024
Indiana University – Purdue University Indianapolis
2020-2024
Nanjing Forestry University
2024
Bacterial infections caused by pathogens have always been a thorny issue that threatens human health, and there is urgent need to develop new generation of antimicrobial nano-agents treatments. Herein, biodegradable nickel disulfide (ND) nanozymes as excellent antibacterial agents integrate photothermal performance, nano-catalysis property, glutathione (GSH)-depleting function successfully constructed. The ND can effectively catalyze the decomposition H2O2 produce ⋅OH, hyperthermia generated...
Sonodynamic therapy, with advantages in large tissue penetration depth and great controllability, is a promising type of non-invasive cancer treatment method. Developing sonosensitizers high reactive oxygen species (ROS) quantum yield the ability to regulate tumor microenvironment achieve enhanced performances sonodynamic therapy would thus be rather attractive. Herein, vanadium (V) doped TiO2 (V-TiO2) nanospindles glutathione-depleting properties are fabricated for therapy. Due doping V...
Gemcitabine resistance is a major obstacle to the effectiveness of chemotherapy in pancreatic ductal adenocarcinoma (PDAC). Therefore, new strategies are needed sensitize cancer cells gemcitabine. Here, we constructed gemcitabine-resistant PDAC and analyzed them with RNA-sequence. Employing an integrated approach involving bioinformatic analyses from multiple databases, TGFB2 identified as crucial gene significantly associated poor gemcitabine therapeutic response. The patient-derived...
An essential prerequisite for in vitro biochemical or structural studies is a construct that amenable to high level expression and purification biochemically "well-behaved". In the field of membrane protein research, use green fluorescent (GFP) monitor optimize heterologous different hosts has radically changed ease streamlining multiplexing testing large number candidate constructs. This achieved by genetically fusing proteins N- C-terminus interest act as reporters which can then be...
Malignant melanoma (MM) is an extremely aggressive and fatal form of skin cancer that primarily affects the bottom layer epidermis associated with poor clinical outcomes. Early-stage MM typically treated through surgical removal, while chemotherapy radiotherapy are common conventional treatment options come harmful side effects. Emerging therapies such as immunotherapy, photodynamic therapy, biologic photothermal therapy present hopeful for due to their effective secure drug delivery...
Enteric neuroimmune interactions in gastrointestinal hypersensitivity responses involve antigen detection by mast cells, cell degranulation, release of chemical mediators, and modulatory actions the mediators on enteric nervous system (ENS). Electrophysiological methods were used to investigate electrical synaptic behavior neurons stomach small intestine during exposure β-lactoglobulin guinea pigs sensitized cow's milk. Application preparations depolarized membrane potential increased...
Mast cells express the substance P (SP) neurokinin 1 receptor and calcitonin gene-related peptide (CGRP) in guinea pig human small intestine. Enzyme-linked immunoassay showed that activation of intramural afferents by antidromic electrical stimulation or capsaicin released SP CGRP from intestinal segments. Electrical evoked slow excitatory postsynaptic potentials (EPSPs) enteric nervous system. The EPSPs were mediated tachykinin receptors. Capsaicin EPSP-like responses suppressed antagonists...
Abstract Reverse transcription‐polymerase chain reaction (RT‐PCR), immunohistochemistry, electrophysiological recording, and intraneuronal injection of the neuronal tracer biocytin were integrated in a study functional expression corticotropin‐releasing factor (CRF) receptors guinea pig enteric nervous system. RT‐PCR revealed CRF 1 receptor mRNA, but not 2 , both myenteric submucosal plexuses. Immunoreactivity for was distributed widely plexus stomach small large intestine intestine....
Lubiprostone activates ClC-2 chloride channels in epithelia. It is approved for treatment of chronic idiopathic constipation adults and constipation-predominate irritable bowel syndrome women. We tested a hypothesis that lubiprostone can reverse the constipating action morphine investigated mechanism action. Short-circuit current (Isc) was recorded Ussing chambers as marker secretion during pharmacological interactions between lubiprostone. Measurements fecal wet weight were used to obtain...
Colorectal cancer (CRC) is ranked as the second leading cause of cancer-related death worldwide. Many abnormally expressed long non-coding RNAs (lncRNAs) in CRC were identified with development next-generation sequencing, most functions which are largely unclear. In this study, we report that lncRNA SLC7A11-AS1 was significantly overexpressed by analyzing TCGA database and 6 pairs clinical samples. High level related to poor overall survival knockdown could inhibit proliferation, migration...
Abstract In the past few decades, advances in outcomes of patients suffering from pancreatic ductal adenocarcinoma (PDAC) have lagged behind these gained treatment many other malignancies. Although pivotal role SUMO pathway PDAC has been illustrated, underlying molecule drivers yet to be fully elucidated. present study, we identified SENP3 as a potential suppressor progression through an vivo metastatic model. Further studies revealed that inhibited invasion system dependent fashion....
Abstract Introduction: This study aimed to assess the association between long-acting injectable (LAI) antipsychotic prescription and risk of psychiatric hospitalization in patients with treatment-resistant schizophrenia (TRS) receiving clozapine. Methods: In this retrospective cohort at a single tertiary center, we analyzed rehospitalization hazard ratios (HRs) refractory patients, classified by DSM-IV-TR DSM-5 criteria. We examined various psychotropic regimens—clozapine or without other...
Immunofluorescence was used to study immunoreactivity (IR) for corticotropin-releasing factor (CRF) in the guinea pig enteric nervous system. CRF-IR expressed both myenteric and submucosal plexuses of all regions large small intestine plexus stomach. nerve fibers were present plexuses, circular muscle coat, surrounding arterioles. Most persisted after 7 days organotypic culture. not coexpressed with tyrosine hydroxylase-IR or calcitonin gene-related peptide-IR fibers. The proportions cell...
Objective: Cancer stem cell marker CD44 and its variant isoforms (CD44v) may be correlated with tumor growth, metastasis, chemo-radiotherapy resistance. However, the prognostic power of CD44v in advanced cancer remains controversial. Therefore, purpose our study was to generalize significance these markers patients. Methods: Hazard ratios (HRs) 95% confidence intervals (95% CIs) were calculated from multivariable analysis assess associations among CD44, CD44v6, CD44v9 positivity overall...
Pancreatic ductal adenocarcinoma (PDAC) is a malignant tumor with poor prognosis. Gemcitabine-based chemotherapy has become one of the main modalities its management. However, gemcitabine resistance frequently occurs, leading to failure PDAC therapy. Platelet-derived growth factors (PDGFs) and their receptors play important roles in cancer progression chemoresistance. We aimed investigate biological function therapeutic significance platelet-derived factor C (PDGFC) drug-resistant PDAC. Our...
Serotonin [5-hydroxytryptamine (5-HT)] is released from enterochromaffin cells in the mucosa of small intestine. We tested a hypothesis that elevation 5-HT environment enteric mast might degranulate and release mediators become paracrine signals to nervous system, spinal afferents, secretory glands. Western blotting, immunofluorescence, ELISA, pharmacological analysis were used study expression receptors by intestine action histamine guinea pig segments human jejunum discarded during...
Novel prognostic markers and therapeutic targets for advanced cancer are urgently needed. This report with trial sequential analysis (TSA) was first conducted to provide robust estimates of the correlation between aldehyde dehydrogenase 1 (ALDH1) Nestin clinical outcomes patients.Hazard ratios (HRs) 95% confidence intervals (CIs) were summarized overall survival (OS), disease-free (DFS), progression-free (PFS), cancer-specific (CSS), relapse/recurrence-free (RFS), metastasis-free (MFS) from...
Expression of hypoxia-inducible factors (HIFs) has been observed, but their prognostic role in advanced cancers remains uncertain. We conducted a meta-analysis to establish the effect HIFs and better guide treatment planning for cancers.Pooled hazard ratios (HRs) 95% confidence intervals (CIs) were calculated. Trial sequential analysis (TSA) was also performed. The clinical outcomes included overall survival (OS), disease-free (DFS), progression-free (PFS), cancer-specific (CSS),...
Abstract Lipid metabolism reprogramming stands as a fundamental hallmark of cancer cells. Unraveling the core regulators lipid biosynthesis holds potential to find promising therapeutic targets in pancreatic ductal adenocarcinoma (PDAC). Here, it is demonstrated that platelet‐derived growth factor C (PDGFC) orchestrated metabolism, thereby facilitated malignant progression PDAC. Expression PDGFC upregulated PDAC cohorts and corelated with poor prognosis. Aberrantly high expression promoted...
Actions of lubiprostone, a selective type-2 chloride channel activator, on mucosal secretion were investigated in guinea pig small intestine and colon. Flat-sheet preparations mounted Ussing flux chambers for recording short-circuit current (Isc) as marker electrogenic secretion. Lubiprostone, applied to the intestinal mucosa eight concentrations ranging from 1-3000 nM, evoked increases Isc concentration-dependent manner with an EC50 42.5 nM. Lubiprostone colon nM 31.7 Blockade enteric...
Background Ca2+/calmodulin-dependent protein kinases (CaMKs) are major downstream mediators of neuronal calcium signaling that regulate multiple functions. CaMKII, one the key CaMKs, plays a significant role in mediating cellular responses to external molecules. Although an essential enteric nervous system (ENS), CaMKII neurogenic intestinal function has not been determined. In this study, we investigated and expression pattern ENS across several mammalian species. Methodology/Principal...