A5042769137- Gastrointestinal motility and disorders
- Neuropeptides and Animal Physiology
- Gastroesophageal reflux and treatments
- Stress Responses and Cortisol
- Diet and metabolism studies
- Ion Channels and Receptors
- Adenosine and Purinergic Signaling
- Neuroendocrine regulation and behavior
- Helicobacter pylori-related gastroenterology studies
- Biochemical Analysis and Sensing Techniques
- Infant Health and Development
- Congenital gastrointestinal and neural anomalies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Ion Transport and Channel Regulation
- Regulation of Appetite and Obesity
- Pediatric Pain Management Techniques
- Asthma and respiratory diseases
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Diabetes Treatment and Management
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Pancreatic function and diabetes
- Ion channel regulation and function
- Vagus Nerve Stimulation Research
University of Wisconsin–La Crosse
2014-2024
Central South University
2022
Third Xiangya Hospital
2022
University of South China
2021
The Ohio State University
2006-2020
BeiGene (China)
2019-2020
Capital Medical University
2019
Ministry of Education of the People's Republic of China
2019
John Wiley & Sons (United Kingdom)
2018
Hudson Institute
2018
SHANK3 (also known as PROSAP2) is a postsynaptic scaffolding protein at excitatory synapses in which mutations and deletions have been implicated patients with idiopathic autism, Phelan–McDermid (aka 22q13 microdeletion) syndrome, other neuropsychiatric disorders. In this study, we created novel mouse model of human autism caused by the insertion single guanine nucleotide into exon 21 (<i>Shank3<sup>G</sup></i>). The resulting frameshift causes premature STOP codon loss major higher...
604 Background: KRAS G12C mutation is an oncogenic driver and a validated therapeutic target in solid tumors. Glecirasib, highly selective, covalent oral inhibitor, has demonstrated promising clinical activity NSCLC CRC; however, the efficacy safety of glecirasib are unknown PDAC other tumors with mutation. Methods: Two phase 1/2 trials (NCT05009329 China; NCT05002270 US, Europe Israel) evaluating patients (pts) tumor harboring mutations. As both have similar inclusion exclusion criteria, we...
Conventional intracellular microelectrodes and injection of biocytin were used to study the actions IL-1β IL-6 on electrical synaptic behavior in morphologically identified guinea pig small intestinal submucous neurons. Exposure nanomolar concentrations either or stimulated neuronal excitability. The excitatory action consisted depolarization membrane potential, decreased conductance, increased discharge potentials. Excitatory was suppressed by natural human receptor antagonist. Electrical...
Hu proteins, together with neurone‐specific enolase (NSE), protein gene product 9.5 (PGP‐9.5), microtubule‐associated protein‐2 (MAP‐2) and tubulin beta III isoform, were evaluated immunohistochemically as neuronal markers in whole‐mount preparations cultures obtained from the myenteric plexus of guinea‐pig small intestine. Anti‐Hu immunostaining marked ganglion cell somas nuclei without staining processes whole‐mounts cultures. The bodies not obscured by multiple fibres this facilitated...
Electrophysiological recording was used to study a type of slow excitatory postsynaptic potential (slow EPSP) that mediated by release ATP and its action at P2Y1 receptors on morphologically identified neurones in the submucosal plexus guinea-pig small intestine. MRS2179, selective purinergic receptor antagonist, blocked both EPSP mimicry exogenously applied ATP. Increased conductance accounted for depolarization phase EPSP, which occurred exclusively with S-type electrophysiological...
Abstract The P2X 7 purinergic receptor subtype has been cloned and emphasized as a prototypic P2Z involved in neurotransmission the central nervous system ATP‐mediated lysis of macrophages immune system. Less is known about neurobiology receptors enteric (ENS). We studied distribution with indirect immunofluorescence used selective agonists antagonists to analyze pharmacologic aspects its electrophysiologic behavior determined intracellular “sharp” microelectrodes patch‐clamp recording...
Enteric neuroimmune interactions in gastrointestinal hypersensitivity responses involve antigen detection by mast cells, cell degranulation, release of chemical mediators, and modulatory actions the mediators on enteric nervous system (ENS). Electrophysiological methods were used to investigate electrical synaptic behavior neurons stomach small intestine during exposure β-lactoglobulin guinea pigs sensitized cow's milk. Application preparations depolarized membrane potential increased...
Mast cells express the substance P (SP) neurokinin 1 receptor and calcitonin gene-related peptide (CGRP) in guinea pig human small intestine. Enzyme-linked immunoassay showed that activation of intramural afferents by antidromic electrical stimulation or capsaicin released SP CGRP from intestinal segments. Electrical evoked slow excitatory postsynaptic potentials (EPSPs) enteric nervous system. The EPSPs were mediated tachykinin receptors. Capsaicin EPSP-like responses suppressed antagonists...
Abstract Reverse transcription‐polymerase chain reaction (RT‐PCR), immunohistochemistry, electrophysiological recording, and intraneuronal injection of the neuronal tracer biocytin were integrated in a study functional expression corticotropin‐releasing factor (CRF) receptors guinea pig enteric nervous system. RT‐PCR revealed CRF 1 receptor mRNA, but not 2 , both myenteric submucosal plexuses. Immunoreactivity for was distributed widely plexus stomach small large intestine intestine....
ATP is a putative inhibitory neurotransmitter responsible for junction potentials (IJPs) at neuromuscular junctions in the intestine. This study tested hypothesis that purinergic P2Y 1 receptor subtype mediates IJPs. IJPs were evoked by focal electrical stimulation myenteric plexus and recorded with “sharp” intracellular microelectrodes circular muscle coat. Stimulation three categories of IJPs: 1) purely IJPs, 2) partially 3) nonpurinergic Purely suppressed selective antagonist MRS2179....
Melanin-concentrating hormone (MCH) is expressed primarily in the hypothalamus and has a positive impact on feeding behavior energy balance. Although MCH gastrointestinal tract, its role this system remains elusive. We demonstrate that, compared to wild type, mice genetically deficient had substantially reduced local inflammatory responses mouse model of experimental colitis induced by intracolonic administration 2,4,6 trinitrobenzene sulfonic acid (TNBS). Likewise, receiving treatments with...
Glucagon-like peptide-2 (GLP-2) is an important neuroendocrine peptide in intestinal physiology. It influences digestion, absorption, epithelial growth, motility, and blood flow. We studied involvement of GLP-2 mucosal secretory behavior. Submucosal-mucosal preparations from guinea pig ileum were mounted Ussing chambers for measurement short-circuit current ( I sc ) as a surrogate chloride secretion. action on neuronal release acetylcholine was determined with ELISA. Enteric expression the...
Lubiprostone activates ClC-2 chloride channels in epithelia. It is approved for treatment of chronic idiopathic constipation adults and constipation-predominate irritable bowel syndrome women. We tested a hypothesis that lubiprostone can reverse the constipating action morphine investigated mechanism action. Short-circuit current (Isc) was recorded Ussing chambers as marker secretion during pharmacological interactions between lubiprostone. Measurements fecal wet weight were used to obtain...
Functional gastrointestinal disorders are those in which no abnormal metabolic or physical processes, can account for the symptoms, be identified. The irritable bowel syndrome (IBS) is a significant functional disorder, affects 10-20 percent of population worldwide. Predominant symptoms IBS defecation associated with abdominal pain, both may exacerbated by psychogenic stress. Our study was designed to test hypothesis that subset patients diagnosis an autoimmune degenerative neuropathy...
Immunofluorescence was used to study immunoreactivity (IR) for corticotropin-releasing factor (CRF) in the guinea pig enteric nervous system. CRF-IR expressed both myenteric and submucosal plexuses of all regions large small intestine plexus stomach. nerve fibers were present plexuses, circular muscle coat, surrounding arterioles. Most persisted after 7 days organotypic culture. not coexpressed with tyrosine hydroxylase-IR or calcitonin gene-related peptide-IR fibers. The proportions cell...
Abstract Background Corticotropin‐releasing factor ( CRF ) mediates our body's overall responses to stress. The role of central in stress‐stimulated colonic motility is well characterized. We hypothesized that transient perturbation expression enteric sufficient change stress‐induced motor and secretory responses. Methods Sprague–Dawley rats (adult, male) were subjected 1‐h partial restraint stress PRS euthanized at 0, 4, 8, 24 h. mRNA peptide levels the colon quantified by real‐time RT ‐...
Serotonin [5-hydroxytryptamine (5-HT)] is released from enterochromaffin cells in the mucosa of small intestine. We tested a hypothesis that elevation 5-HT environment enteric mast might degranulate and release mediators become paracrine signals to nervous system, spinal afferents, secretory glands. Western blotting, immunofluorescence, ELISA, pharmacological analysis were used study expression receptors by intestine action histamine guinea pig segments human jejunum discarded during...