A5012059639- Ion channel regulation and function
- Ion Transport and Channel Regulation
- RNA Research and Splicing
- Retinoids in leukemia and cellular processes
- Cutaneous lymphoproliferative disorders research
- Bone and Dental Protein Studies
- Cancer Research and Treatments
- Receptor Mechanisms and Signaling
- Cellular Mechanics and Interactions
- Lymphoma Diagnosis and Treatment
- Adipose Tissue and Metabolism
- T-cell and Retrovirus Studies
- dental development and anomalies
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Ion Channels and Receptors
- Lung Cancer Treatments and Mutations
- Diet, Metabolism, and Disease
- Melanoma and MAPK Pathways
- RNA regulation and disease
- Alcohol Consumption and Health Effects
- Pharmacological Effects and Toxicity Studies
- Drug Transport and Resistance Mechanisms
- Cancer-related gene regulation
- Neuroscience and Neuropharmacology Research
Virginia Commonwealth University
2022-2024
University of Alabama at Birmingham
2008-2022
University of Alabama
2009
Pediatric Nephrology of Alabama
2009
National Defense Medical Center
2002-2003
Regulated mRNA decay is a highly important process for the tight control of gene expression. Inherently unstable mRNAs contain AU-rich elements (AREs) in 3′ untranslated regions that direct rapid by interaction with decay-promoting ARE-binding proteins (ARE-BPs). The ARE-containing regulated signaling pathways are believed to directly target ARE-BPs. Here, we show BRF1 involved ARE-mediated (AMD) phosphorylated MAPK-activated protein kinase 2 (MK2). In vitro assays using different fragments...
Lung cancer is the third most common with Black/AA men showing higher risk and poorer outcomes than NHW men. disparities are multifactorial, driven by tobacco exposure, inequities in care access, upstream health determinants, molecular determinants including biological genetic factors. Elevated expressions of protein arginine methyltransferases (PRMTs) correlating prognosis have been observed many cancers. Most importantly, our study shows that PRMT6 displays expression lung tissues compared...
Cyclosporine A (CsA) is an efficient immunosuppressant used for reducing allograft rejection but with a severe side effect of causing hypertension. We hypothesize that the renal epithelial sodium channel (ENaC) may participate in CsA-induced In present study, we patch-clamp cell-attached configuration to examine whether and how CsA stimulates ENaC A6 distal nephron cells. The data showed significantly increased open probability. Since inhibitor ATP-binding cassette A1 (ABCA1) transporter,...
Myofibroblasts are implicated in pathological stromal responses associated with lung fibrosis. One prominent phenotypic marker of fully differentiated myofibroblasts is the polymerized, thick cytoplasmic filaments containing newly synthesized α-smooth muscle actin (α-SMA). These α-SMA-containing important for myofibroblast contractility during tissue remodeling. However, molecular mechanisms regulating formation and maturation have not been defined. This study demonstrates a critical role...
Molecular genetic studies have indicated that alcohol dehydrogenase may be involved in the synthesis of retinoic acid, a hormonal molecule regulating diverse cellular functions at transcriptional level. Class IV (ADH) has been reported to most efficient enzyme catalyzing oxidation retinol human ADH family. Initial velocity, product inhibition, and dead-end inhibition experiments were performed with recombinant class elucidate kinetic mechanism all-trans-retinol all-trans-retinal as natural...
Hepatic lipid metabolism is controlled by integrated metabolic pathways. Excess accumulation of hepatic TG a hallmark nonalcoholic fatty liver disease, which associated with obesity and insulin resistance. Here, we show that KH-type splicing regulatory protein (KSRP) ablation reduces levels diet-induced hepatosteatosis. Expression period 2 (Per2) increased during the dark period, circadian oscillations several core clock genes are altered delayed phase in Ksrp(-/-) livers. Diurnal expression...
The study evaluated whether SPP1/osteopontin (OPN) splice variants are differentially expressed in nonmelanoma skin cancer compared to normal skin. absolute number of mRNA molecules OPN-a predominated and OPN-b, OPN-c, OPN-5. However, mRNAs OPN-a, OPN-c were higher levels cutaneous squamous cell carcinomas (cSCCs) basal relative Additionally, OPN-5 expression was than OPN-b cancer, respectively. Furthermore, we identified four variants, which cloned analyzed for protein expression.
mRNA decay mediated by the AU-rich elements (AREs) is one of most studied post-transcriptional mechanisms and modulated ARE-binding proteins (ARE-BPs). To understand regulation K homology splicing regulatory protein (KSRP), a decay-promoting ARE-BP, we purified KSRP complexes identified an RNA helicase, DDX1. We showed that down-regulation DDX1 expression elevated cytoplasmic levels facilitated ARE-mediated decay. Association with 14-3-3 proteins, are predominately located in cytoplasm,...
The epithelial sodium channel (ENaC) in cortical collecting duct (CCD) principal cells plays a critical role regulating systemic blood pressure. We have previously shown that cholesterol (Cho) the apical cell membrane regulates ENaC; however, underlying mechanism remains unclear.Patch-clamp technique and confocal microscopy were used to evaluate ENaC activity density.Here we show extraction of Cho with methyl-β-cyclodextrin (MβCD) significantly reduced amiloride-sensitive current...
Compound 1 is a potent rexinoid that highly effective in cancer chemoprevention but elevates serum triglycerides. In an effort to separate the lipid toxicity from anticancer activity of 1, we synthesized four new analogs which three rexinoids did not elevate Rexinoids 3 and 4 are twice as binding Retinoid X receptor (RXR). All-trans retinoic acid (ATRA) plays key role maintaining skin homeostasis, 3–6 upregulating genes responsible for biosynthesis ATRA. Inflammation cancer, diminishing...
Abstract Lung cancer continues to be the leading cause of death in United States. Despite recent advances, five-year survival rate for lung compared other cancers still remains fairly low. The discovery molecular targets is key development new approaches and therapies. Electrically silent voltage-gated potassium channel (KvS) subfamilies, which are unable form functional homotetramers, implicated cell-cycle progression, cell proliferation tumorigenesis. Here, we analyzed expression KvS...
The chromatoid body (CB) is a specific cloud-like structure in the cytoplasm of haploid spermatids. Recent findings indicate that CB identified as male germ cell-specific RNA storage and processing center, but its function has remained elusive for decades. In somatic cells, KH-type splicing regulatory protein (KSRP) involved regulating gene expression maturation select microRNAs (miRNAs). However, KSRP spermatogenesis remains unclear. this study, we showed partly localizes CB, component CB....
Lung cancer is a leading cause of cancer-related deaths worldwide. Black/African American (B/AA) populations, in particular, exhibit the highest incidence and mortality rates lung adenocarcinoma (LUAD) United States.
Abstract Non-Hispanic Black/African American (Black/AA) men in the United States have disproportionally higher incidence and mortality rates of lung cancer compared to non-Hispanic White (NHW) men. Molecular determinants including biological genetic factors are believed play critical roles driving disparities. Yet, several recent large-scale genomic studies fail identify significant somatic differences driver genes contributing observed disparities between Black/AA NHW groups. These findings...
Abstract Cutaneous T-cell lymphomas (CTCLs) are a group of non-Hodgkin diseases characterized by localization neoplastic T-cells in the skin. The most common forms CTCLs mycosis fungoides (MF) and Sézary syndrome (SS). Bexarotene (Targretin®) is currently only FDA approved retinoid X receptor (RXR) -selective agonist (rexinoid) for treatment CTCLs. Despite its low toxicity compared to retinoids other chemotherapeutic agents, it induces hypothyroidism markedly elevates serum triglycerides...
We and others have previously shown that the epithelial sodium channel (ENaC) is stimulated by anionic phospholipids (APs) such as phosphatidylinositol‐4,5‐bisphosphate (PI(4,5)P 2 ) phosphatidylinositol‐3,4,5‐trisphosphate (PI(3,4,5)P 3 ). Although functional studies suggest β‐ γ‐ENaC interact with PI(4,5)P PI(3,4,5)P , respectively, however, whether ENaC selectively binds to over other APs has not been carefully examined. By using newly developed PIP Strips™ different cell models, we found...
Cutaneous T-cell lymphomas (CTCLs) are a group of non-Hodgkin diseases characterized by localization neoplastic T-cells in the skin. The most common forms CTCLs mycosis fungoides (MF) and Sézary syndrome (SS). Bexarotene (Targretin®) is currently only FDA approved retinoid X receptor (RXR) -selective agonist (rexinoid) for treatment CTCLs. Despite its low toxicity compared to retinoids other chemotherapeutic agents, it induces hypothyroidism markedly elevates serum triglycerides (TGs). Thus,...