A5019116719- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 and COVID-19 Research
- Veterinary medicine and infectious diseases
- Influenza Virus Research Studies
- Cystic Fibrosis Research Advances
- Monoclonal and Polyclonal Antibodies Research
- Diphtheria, Corynebacterium, and Tetanus
- Peptidase Inhibition and Analysis
- Advanced Drug Delivery Systems
- Metabolomics and Mass Spectrometry Studies
- Complement system in diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Antibiotic Resistance in Bacteria
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Inhalation and Respiratory Drug Delivery
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Protein purification and stability
- Tracheal and airway disorders
- Iron Metabolism and Disorders
- Mycobacterium research and diagnosis
Fundação Oswaldo Cruz
1994-2023
Abstract Purpose After nearly 3 years of the COVID-19 pandemic, even though a vast body knowledge and products (including vaccines treatments) have been developed disseminated, virus is still evolving new variants arising. Consequently, thousands lives continue to be lost. Neutralizing monoclonal antibodies (nAbs) are promising drugs that emerged treat SARS-CoV-2. In uncertainty current situation, there question whether organizations should invest in this technology. To help decision-making...
In Brazil, polysaccharide–protein conjugate vaccine against Neisseria meningitidis group C (MenCPS-TT) using hydrazine-activated-tetanus toxoid (TT) as a carrier protein has been developed. Because of the toxicity hydrazine in humans, it is necessary to monitor this substance's process control step during production. The electroanalytical methodology was developed and validated for determination MenCPS-TT production by differential pulse polarography. reduction potential −0.95 V acetone...
Neisseria meningitidis group C is an encapsulated bacterium that causes several diseases and associated with high mortality rates, thereby constituting a serious public health problem. Bio-Manguinhos/Fiocruz developing conjugate vaccine by covalent attachment of capsular polysaccharide to hydrazide-activated tetanus toxoid through reductive amination. It necessary quantify free components as quality control process prevent exacerbated adverse reactions and/or attenuation immunogenicity....
Objectives: This study aims the development of a method for free protein separation and quantitation in meningococcal C conjugate vaccine developed by Bio-Manguinhos, accordance with WHO requirements.
Objectives: This work is related to comparative analysis between obtained data from pilot and industrial scale of the meningococcal C conjugate vaccine under GMP.
Introduction:The reductive amination chemistry is a synthetic approach normally used in the development of conjugate vaccines.In this approach, high carrier protein reactivity desirable.This improvement occurs by converting side carboxylate groups aspartic and glutamic residues into their respective hydrazides.The measurement conversion an analytical challenge due to sample complexity low concentrations analytes.The determination relative activation rate will allow us monitorize...
1Fiocruz/Bio-Manguinhos; 2Without bond Institutional, with personal interest in the area
Introduction: MDR bacteria causes nosocomial and community-acquired infections associated with immunosuppressed patients.As few antibiotics are effective to treat these infections, the development of new therapies becomes important.Aptamers molecules that bind a specific target molecule potential therapeutic use control infections.Surface proteins possible for aptamers should be conjugated polyethylene glycol (PEGylation) increase their plasma halflife clinical efficacy.Objective: The...