Melanoma is the most lethal malignancy in skin cancer and may occur at any site express melanocytes. Due to malignant melanoma's invasion migration nature, conventional therapies make it challenging remove whole tumor tissue while undertaking high risks of recurrence. Regarding emerging targeted immunotherapy, drug resistance low immunotherapeutic activity remain significant challenges. It thus becoming urgently important develop alternative strategies for melanoma therapy. Herein, a novel...

Chemical conversion of CO2 is providing an opportunity to mitigate the global warming induced by overconsumption fossil fuel. Cu has been regarded as one most powerful contenders in catalyzing conversion, yet precise manipulation its surface state and nearby chemical environment continues pose a formidable challenge. In this work, we report high-efficiency catalyst utilizing CeO2 pure silicon zeolite (S1) co-activate species. CO2-to-methanol (CTM) space-time yield methanol (STYMeOH) obtained...

Significance This paper demonstrates that aminoglycoside antibiotics induce the production of Ap4A in bacteria. Increased intracellular Ap4A, turn, promotes bacterial cell killing by this class antibiotics, which correlated well with elevated damage to membrane upon treatment. These findings reveal a striking connection between and particularly under conditions oxidative stress. Importantly, results study suggest targeting degradation or inducing its hypersynthesis during therapy...

Abstract PIWI-interacting RNAs (piRNAs) are small noncoding that regulate gene expression, yet their molecular functions in neurobiology unclear. While investigating neurodegeneration mechanisms using human α-syn(A53T) Tg and Aβ ;α-syn(A53T) pan-neuronal overexpressing strains, we unexpectedly observed dysregulation of piRNAs. RNAi screening revealed knock down piRNA biogenesis genes improved thrashing behavior; further, a tofu-1 deletion ameliorated phenotypic deficits transgenic strains....

Methicillin-resistant Staphylococcus aureus (MRSA) is a refractory pneumonia-causing pathogen due to the antibiotic resistance and characteristics of persisting inside its host cell. Lysostaphin typical bacteriolytic enzyme for degrading bacterial cell walls via hydrolysis pentaglycine cross-links, showing potential combat multidrug-resistant bacteria. However, there are still grand challenges native lysostaphin because poor shelf stability limited bioavailability. To tackle these...

Abstract Chemical conversion of CO 2 is providing an opportunity to mitigate the global warming induced by overconsumption fossil fuel. Cu has been regarded as one most powerful contenders in catalyzing conversion, yet precise manipulation its surface state and nearby chemical environment continues pose a formidable challenge. In this work, we report high‐efficiency catalyst utilizing CeO pure silicon zeolite (S1) co‐activate species. ‐to‐methanol (CTM) space‐time yield methanol (STY MeOH )...

Copper has emerged as a promising target for cancer therapy, with extensive studies on copper accumulation-induced cuproptosis. However, the potential of depletion-induced cuproptosis remains largely unexplored. Recently, Zhou et al. (M. Zhou, F. Muhammad, Y. Zhang, T. Li, J. Feng, Zhao and H. Wei, Chem. Sci., 2025, https://doi.org/10.1039/D4SC04712E) reported an innovative strategy depletion-based Notably, this approach leverages solubility product principle, mechanism not previously...

ABSTRACT Cancer is one of the leading causes death worldwide, making pursuit effective treatments to prolong patient survival a major concern in modern medicine. Rare‐earth‐based nanomaterials (RENMs) possess unique optical properties, catalytic and high X‐rays absorption capabilities, offering diverse approaches for cancer therapy. Herein, this review highlights various strategies treatment by exploiting physical chemical properties RENMs. Their fluorescent can be harnessed fluorescence...

Clathrin coats vesicles in all eukaryotic cells and has a well-defined role endocytosis, moving molecules away from the plasma membrane. Its function on routes towards membrane was only recently appreciated is thought to be limited basolateral transport. Here, an unbiased RNAi-based tubulogenesis screen identifies of clathrin (CHC-1) its AP-1 adaptor apical polarity during de novo lumenal biogenesis C. elegans intestine. We show that CHC-1/AP-1-mediated polarized transport intersects with...

Reductive cleavage of alkenes is rarely reported in synthetic chemistry. Here we report a unique H2S-mediated reductive C[double bond, length as m-dash]C bonds under mild conditions, which successful new strategy for the design probes effective sensing H2S with turn-on dual-color fluorescence. A short series phenothiazine ethylidene malononitrile derivatives were shown to react H2S, via intramolecular cyclization reactions form thiophene rings. Enlightened by this reaction mechanism, four...

Though palbociclib, a cyclin-dependent kinases 4 and 6 (CDK4/6) inhibitor has been approved for treating breast cancer, two major clinical challenges remain: (i) Triple-negative cancer (TNBC) appears to be more resistant (ii) Palbociclib-induced senescence-associated secretory phenotype (SASP) pro-tumorigenic function. Here we report that combining palbociclib with the STAT3 nifuroxazide uncouples SASP production from cell cycle exit. Moreover, identified as CDK2 synergistically promotes...

Resveratrol is one of the most studied plant secondary metabolites owing to its numerous health benefits. It accumulated in some plants following biotic and abiotic stress pressures, including UV-C irradiation. Polygonum cuspidatum represents major natural source concentrated resveratrol but underlying mechanisms as well effects irradiation on content have not yet been documented. Herein, we found that significantly increased by 2.6-fold 1.6-fold irradiated leaf samples followed a dark...

Sphingolipids (SLs) serve as structural and signaling molecules in regulating various cellular events growth.Given that SLs contain bioactive species possessing distinct roles, quantitative analysis of sphingolipidome is essential for elucidating their differential requirement during development.Herein we developed a comprehensive sphingolipidomic profiling approach using liquid chromatography-mass spectrometry coupled with multiple reaction monitoring mode (LC-MS-MRM).SL C. elegans revealed...

The ACK family tyrosine kinase SID-3 is involved in the endocytic uptake of double-stranded RNA. Here we identified as a previously unappreciated recycling regulator Caenorhabditis elegans intestine. RAB-10 effector EHBP-1 required for endosomal localization SID-3. Accordingly, animals with loss phenocopied defects observed ehbp-1 and rab-10 single mutants. Moreover, detected sequential protein interactions between EHBP-1, SID-3, NCK-1, DYN-1. In absence DYN-1 failed to localize at tubular...

Antibiotic resistance has become a global crisis. Studies on the mechanism of bacterial tolerance to antibiotics will not only increase our conceptual understanding death but also provide potential targets for novel inhibitors. We screened mutant library containing full set in-frame deletion mutants Escherichia coli K-12 and identified 140 genes that possibly contribute gentamicin tolerance. The ksgA increased inhibition killing potency against mid-log-phase bacteria by aminoglycosides....

Hereditary sensory and autonomic neuropathy Type 1 (HSAN1) is a rare autosomal dominantly inherited neuropathy, clinically characterized by loss of distal peripheral motoneuronal function. Mutations in subunits serine palmitoyltransferase (SPT) have been linked to the majority HSAN1 cases. SPTs catalyze condensation l-serine with palmitoyl-CoA, first committed rate-limiting step de novo sphingolipid biosynthesis. Despite extensive investigation, molecular pathogenesis remains controversial....

Elucidation of host-pathogen interaction is essential for developing effective strategies to combat bacterial infection. Dual RNA-Seq using cultured cells or tissues/organs as the host pathogen has emerged a novel strategy understand responses concurrently from both and at cellular level. However, infection mostly causes systematic organism level where interplay urgently be understood but inevitably being neglected by current practice. Here, we developed an approach that simultaneously...

Visualizing mRNA in real time vivo at high resolution is critical for a full understanding of the spatiotemporal dynamics gene regulation and function. Here, using PP7/PCP-based mRNA-tagging approach, we construct collection tissue-specific differentially expressed toolkit strains visualizing mRNAs encoding apical, basolateral, junctional proteins Caenorhabditis elegans epithelia. We precisely delineate organization these transcripts across multiple subcellular compartments tissues....