A5063827864- Orthopedic Infections and Treatments
- Central Venous Catheters and Hemodialysis
- Clinical Nutrition and Gastroenterology
- Orthopaedic implants and arthroplasty
- Antimicrobial Resistance in Staphylococcus
- Surgical site infection prevention
- Total Knee Arthroplasty Outcomes
- Bacterial Identification and Susceptibility Testing
- Nosocomial Infections in ICU
- Hemodynamic Monitoring and Therapy
- Urinary Tract Infections Management
- Infective Endocarditis Diagnosis and Management
- Pancreatitis Pathology and Treatment
- Childhood Cancer Survivors' Quality of Life
- Inflammatory Bowel Disease
- Blood transfusion and management
- Antifungal resistance and susceptibility
- Streptococcal Infections and Treatments
- Pancreatic and Hepatic Oncology Research
- Dental materials and restorations
- Probiotics and Fermented Foods
- Reproductive tract infections research
- Neonatal Respiratory Health Research
- Sarcoma Diagnosis and Treatment
- Pharmaceutical studies and practices
Hospital General Universitario Gregorio Marañón
2022-2025
Hospital Universitario 12 De Octubre
2012-2024
Museo Nacional de Ciencias Naturales
2021
Consejo Superior de Investigaciones Científicas
2021
Instituto de Ciencias Agrarias
2021
Research Institute Hospital 12 de Octubre
2015-2020
Universidad Complutense de Madrid
2020
Hospital Obispo Polanco
2004
An expanding number of monogenic defects have been identified as causative severe forms very early-onset inflammatory bowel diseases [VEO-IBD]. The present study aimed at defining how next-generation sequencing [NGS] methods can be used to improve identification known molecular diagnosis and adapt treatment.A total 207 children were recruited in 45 paediatric centres through an international collaborative network [ESPGHAN GENIUS working group] with a clinical presentation VEO-IBD [n = 185]...
ABSTRACT Objectives: The aim of the study was to describe experience with teduglutide several Spanish hospitals in pediatric patients SBS (SBS). Methods: Seventeen intestinal failure associated were treated teduglutide. Patients received 0.05 mg · kg −1 day subcutaneous Patients’ demographics and changes parenteral nutrition (PN) needs, fecal losses, citrulline level initially at 3, 6, 12 months collected, as well any adverse events. Results: receiving 55 ml 33 kcal supplementation on...
Oritavancin (ORT) is a new single-dose lipoglycopeptide showing in vitro activity against staphylococci and vancomycin-resistant enterococci. However, there no data regarding its potential use as catheter lock solution are scarce. We constructed an model to analyze the efficacy stability of ORT biofilm enterococci over 7 days at 37 °C. used Staphylococcus aureus, epidermidis, vancomycin-susceptible Enterococcus faecalis ATCC strains. performed metabolic assay using 2-mg/ml 7-day incubation...
ABSTRACT Klebsiella pneumoniae is a significant healthcare-associated pathogen, notable for its diverse virulence and antibiotic resistance profiles. This study aimed to characterize the genotypic phenotypic diversity of K. isolates evaluate their using Galleria mellonella model. Biomass production, metabolic activity, capsule formation, siderophore production were assessed in 27 K . from hospital-associated infections. Lethality curves generated G. model, with survival monitored hourly 16...
The Latin American Deep Sea Exploration Network (LADEN) is an initiative aimed at building a collaborative, interdisciplinary, and sustainable network across America to explore, protect, sustainably manage deep-sea ecosystems. concept of LADEN based on the recent Blue Initiative that forms global platform for research cooperation, mobilization, funding equitable capacity sharing. This will leverage region’s local expertise, international partnerships, philanthropic support ensure countries,...
Background Despite the pathogenesis of vulvovaginal candidiasis (VVC) is multifactorial, this study aimed to assess whether phenotypic characteristics, such as biofilm production and quality, along with clinical symptoms, are associated recurrent VVC (RVVC). Methods Over 1 year (Oct 2021–Oct 2022), we prospectively included 271 patients ≥18 years who attended our institution, had Candida spp. isolated in vaginal swabs, provided informed consent. Patients were followed for year. isolates...
ABSTRACT The differential time to positivity (DTTP) technique is the recommended conservative procedure diagnose catheter-related bloodstream infection (C-RBSI). However, its reliability and accuracy remain under debate. Therefore, we aimed compare DTTP feasibility detect C-RBSI compared catheter culture (CC) method. We conducted a 9-month retrospective study including bacteremic episodes in which both blood cultures (BC) CC were obtained. analyzed diagnostic validity of for detecting gold...
The aim of this study was to analyze the usefulness physical examination, C-reactive protein (CRP), procalcitonin (PCT), white blood cell (WBC) count, and absolute neutrophils counts (ANCs) for diagnosis invasive bacterial infections (IBIs) potentially serious in infants younger than age 3 months presenting with fever without source (FWS) emergency department (ED).A descriptive retrospective that includes all aged who presented FWS ED between July 2008 January 2012. We evaluated diagnostic...
Aim: to communicate home parenteral nutrition (HPN) data obtained from the HPN registry of NADYA-SENPE group (www.nadya-senpe.com) for year 2018 Material and methods: descriptive analysis collected adult pediatric patients with in January 1st, December 31st, 2018. Results: there were 278 45 Spanish hospitals (54.7% women), 23 children 255 adults, which represent a prevalence rate 5.95 patients/million inhabitants/year The most frequent diagnosis adults was "palliative cancer" (22.0%),...
In the practice of breast augmentation and reconstruction, implant irrigation with various solutions has been widely used to prevent infection capsular contracture, but date, there is no consensus on optimal protocol use. Recently, application povidone iodine (PI) for 30 min shown in vitro be most effective irrigating formula reducing contamination smooth implants. However, as not feasible intraoperatively, it necessary determine whether shorter times could equally well test both textured We...
Background Tranexamic acid (TXA) is an antifibrinolytic agent applied in orthopedic surgery and has been proven to reduce post-surgery infection rates. We previously showed that TXA also had additional direct antimicrobial effect against planktonic bacteria. Therefore, we aimed evaluate whether it a synergistic if combination with antibiotics. Materials Methods Three ATCC seven clinical strains of staphylococci were tested serial dilutions vancomycin gentamicin alone at 10 50 mg/ml. The...
Tranexamic acid (TXA) is extensively used in orthopedic surgery and traumatology as an antifibrinolytic agent to control intra- postoperative bleeding and, therefore, indirectly, reduce postsurgery infection rates. The hypothesis of additional antibiotic effect against microorganisms associated with periprosthetic joint needs be further evaluated. We aimed assess whether TXA could bacterial growth using vitro model. ATCC clinical strains staphylococci Cutibacterium acnes were tested both...
Irrigation and debridement using an irrigation solution is a fundamental step during the surgical treatment of both acute chronic periprosthetic joint infection (PJI). However, there no consensus on optimal solution, nor sufficient evidence time combination solutions. Therefore, it necessary to determine which or solutions most efficacious against biofilm, as well time. We conducted experimental in vitro model by inoculating stainless steel discs with ATCC strains methicillin-susceptible...
We assessed the success rate of vancomycin catheter lock therapy (VLT) in combination with systemic antimicrobials patients staphylococcal catheter-related bloodstream infection (C-RBSI). Over a 6-year period, we retrospectively collected clinical and microbiological data from long-term central venous catheters C-RBSI who were treated VLT. then VLT based on two criteria: 1) retention time> 3 months 2) place until end use. found 217 episodes, 115 (53.0%) which managed conservative therapy. Of...
Vulvovaginal candidiasis (VVC) is caused by biofilm formation and epithelial invasion. In addition, Escherichia coli (EC) can establish a vaginal intracellular reservoir modulating Candida spp. production. We aimed to analyze the behavior of albicans (CA) EC both in single cultures co-cultures.We prospectively collected CA isolates from swabs over 6 months. selected positive with (cases) comparator group either or (controls). analyzed overall biomass production metabolic activity co-cultures...
Aim: to communicate the home parenteral nutrition (HPN) data obtained from HPN registry of NADYA-SENPE group (www.nadya-senpe.com) for year 2019. Material and methods: a descriptive analysis collected adult pediatric patients with in January 1 December 31, Results: total 283 (51.9 % women), 31 children, 252 adults 47 Spanish hospitals were registered, which represents prevalence rate 6.01 per million inhabitants The most frequent diagnosis was "palliative oncological" "others" (21.0 %). In...
Irrigation and debridement using an irrigation solution is a fundamental step during the surgi-cal treatment of both acute chronic periprosthetic joint infection (PJI). However, there no consensus on optimal solution, nor sufficient evidence time combination solutions. Therefore, it necessary to determine which or combi-nation solutions most efficacious against biofilm, as well time. We conducted experimental in vitro model by inoculating stainless steel discs with ATCC strains...
Background The differential time to positivity (DTTP) technique is recommended for the conservative diagnosis of catheter-related bloodstream infection (C-RBSI). based on a 120-minute difference between microbial growth in blood drawn through catheter and peripheral vein. However, this cut-off has failed confirm C-RBSI caused by Candida spp. Staphylococcus aureus . Objective We hypothesized that biofilm both microorganisms disperses faster than other load rapidly equalized blood. Therefore,...
Antibiotic-loaded bone cement is the most widely used approach for treatment of biofilm-induced septic sequelae in orthopedic surgery. Dalbavancin a lipoglycopeptide that acts against Gram-positive bacteria and has long half-life, so we aimed to assess whether it could be new alternative drug antibiotic-loaded periprosthetic joint infections. We assessed elution capacity dalbavancin compared with vancomycin cement. Palacos®R (Heraeus Medical GmbH, Wehrheim, Germany) was manually mixed each...
Arthropods and specifically beetles can synthesize and/or sequester metabolites from dietary sources. In beetle families such as Tenebrionidae Meloidae, a few studies have reported species with toxic defensive substances antiparasitic properties that are consumed by birds. Here we studied the activity of extracts present in habitat Great Bustard (Otis tarda) against four pathogen models (Aspergillus niger, Meloidogyne javanica, Hyalomma lusitanicum, Trichomonas gallinae). The insect...
The traditional method for assessing the capacity of a microorganism to produce biofilm is generally static in vitro model multi-well plate using crystal violet (CV) binding assay, which takes 96 h. Furthermore, while simple perform, its reproducibility poor.We evaluated whether matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) could make it possible differentiate between high-and low-biofilm-producing microorganisms on 24-h cultures Staphylococcus...
We previously demonstrated the efficacy of a frozen dalbavancin-heparin (DH) lock solution against biofilms staphylococci. However, as enterococci also commonly cause catheter-related bloodstream infections (C-RBSI), we assessed bioactivity dalbavancin (D) and DH enterococci. Over 6 months, compared (1 mg/ml) with that D in terms cfu counts metabolic activity Enterococcus faecalis faecium (four strains each). For each solution, individually results obtained at time point (months 3 6)...