A5063749705- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Endometriosis Research and Treatment
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Uterine Myomas and Treatments
- Prenatal Screening and Diagnostics
- Gynecological conditions and treatments
- Endometrial and Cervical Cancer Treatments
- Epilepsy research and treatment
- Immune cells in cancer
- Preterm Birth and Chorioamnionitis
- Pregnancy and preeclampsia studies
- COVID-19 Impact on Reproduction
- Periodontal Regeneration and Treatments
- Glioma Diagnosis and Treatment
- Reproductive tract infections research
- Estrogen and related hormone effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Biomarkers in Disease Mechanisms
- Reproductive biology and impacts on aquatic species
- Pregnancy and Medication Impact
- Assisted Reproductive Technology and Twin Pregnancy
Women’s Health Care
2024
Sofia University "St. Kliment Ohridski"
2023
Alexandrovska Hospital
2021
Background: The Notch signaling pathway is an important regulator of stem cell activity in various tissues, including the central nervous system. It has been implicated neurodevelopmental processes, neuronal differentiation and synaptic plasticity. Research suggests that its expression may be associated with certain epileptogenic lesions, particularly those origin. aim this study was to investigate Notch-1 brain biopsies from cases pharmacoresistant epilepsy. Methods: Here, we used...
The functionality of human spermatozoa is a key factor for the success rate natural reproduction, but unfortunately infertility progressively increases due to multifarious environmental factors. Such disquieting statistics requires employment sophisticated computer-assisted methods semen quality analysis, whose precision, however, unreliable in cases patients with low sperm concentrations. In this study, we report novel quartz crystal microbalance (QCM) based biosensor in-situ assessment...
Adenomyosis and endometriosis are distinct gynecological disorders characterized by ectopic growth of endometrial tissue. Their etiology remains unclear, but stem cells have been implicated in both. The aim this study was to investigate compare the quantity NOTCH1+ CD117+ adenomyosis lesions. Immunohistochemical staining endometrium biopsies using antibodies against NOTCH1 CD117 performed. spatial distribution stromal positive for these markers were determined compared between Additionally,...
Abstract Background Sperm abnormalities may negatively affect embryo development. Objectives To determine the influence of sperm (morphology, motility, DNA fragmentation) on morphokinetic variables and clinical outcome conventional IVF. Materials methods Participants were 86 couples undergoing in vitro fertilization (IVF). morphology was evaluated according to strict criteria proposed by Kruger/Tygerberg. CASA system applied for motility assessment. fragmentation assessed chromatin structure...
Sperm DNA fragmentation (SDF) and sperm morphological defects can negatively affect ART outcomes. Consequently, there is a need for additional semen processing technique that accounts status morphology prior to ICSI. The objective was evaluate the efficacy of an zona pellucida adhesion-based selection obtaining populations with high percentage normal integrity as compared native routine swim-up preparation. Semen samples from 78 normozoospermic men were subjected swim up placed in petri...
Platelet-rich plasma (PRP) is used for successful regeneration of female reproductive tissues. However, little known about the effect ovarian PRP treatment on oocyte and embryo quality. The objective our study was to assess role autologous reserve number quality oocytes embryos in women with poor response (POR) undergoing in-vitro fertilization cycles. A total 66 POR were treated injection two subsequent menstrual antral follicle counts, serum anti-Mullerian hormone, follicle-stimulating...
The aim of this prospective study was to compare the endometrial microbiome between pregnant and non-pregnant women after frozen embryo transfer (FET) with euploid embryos. Endometrial biopsies were collected from 30 during mid-luteal phase in a natural cycle. FET performed embryos up 3 months biopsy. microbiota composition analysed using 16S rRNA (v4-v5 region) next generation sequencing (NGS). analysis different clinical outcomes biopsy (no pregnancy (n = 14), ultrasound confirmed 16))...
Zona pellucida is one of the main selective barriers for spermatozoa before reaching oocyte. Using native zona in sperm selection prior to Intracytoplasmic Sperm Injection (ICSI) has been proven effective but inconvenient approach In Vitro Fertilisation (IVF) laboratory. The application autologous solubilised zonae pellucidae ICSI not studied yet.
Abstract The present study aimed to identify, quantify and compare the quantities of very small embryonic-like (VSEL) stem cells in two main fractions fresh umbilical cord blood (UCB). To do this, UCB obtained during caesarean section underwent gradient centrifugation. Nucleated isolated from – mononuclear cell layer above gradient, red pellet below were immunofluorescently labelled identify VSELs (CD45 − /CD133 + /SSEA4 ). These then analysed via multiparameter sorting on a flow cytometer...
Herein, we report the first case of mild malformation cortical development with oligodendroglial hyperplasia and epilepsy (MOGHE) in Bulgaria. It is a newly recognised clinico-pathological entity medically intractable focal paediatric patients. The patient interest 9-year-old boy who has been suffering from refractory since age three. Positron emission tomography revealed consistent hypometabolism maximum orbitofrontal fronto-opercular cortex, as well adjacent anterior insula temporal...
The objective of this study was to compare the motility, morphology, and levels DNA fragmentation spermatozoa subjected conventional swim-up or cumulus matrix (CM) sperm selection. Semen samples were collected from 60 normozoospermic men at a private hospital between December 2021 March 2022. After liquefaction, semen separated into two portions – one part preparation, remaining CM obtained by mechanical isolation healthy donor oocytes. analysis evaluation performed according WHO 6th Edition...
Abstract Study question Are there any quantitative associations between endometrial macrophages populations and microorganisms’ diversity? Summary answer The quantities spatial distribution of correlated significantly with several microbiome diversity indices. What is known already Numerous studies have linked the relative abundance Lactobacilli a receptive endometrium, while presence multiple other bacterial species has been implicated in implantation failure. Another determinant...