A5085256543- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Endometriosis Research and Treatment
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Gynecological conditions and treatments
- Uterine Myomas and Treatments
- Prenatal Screening and Diagnostics
- Preterm Birth and Chorioamnionitis
- Pregnancy and preeclampsia studies
- Periodontal Regeneration and Treatments
- Epilepsy research and treatment
- Ectopic Pregnancy Diagnosis and Management
- Endometrial and Cervical Cancer Treatments
- Assisted Reproductive Technology and Twin Pregnancy
- Evolutionary Algorithms and Applications
- Sexual Differentiation and Disorders
- COVID-19 Impact on Reproduction
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Biomarkers in Disease Mechanisms
- Immune cells in cancer
- Renal and related cancers
Alexandrovska Hospital
2021-2024
Bulgarian Academy of Sciences
2021-2024
Institute of Information and Communication Technologies
2021-2024
Women’s Health Care
2024
Background: The Notch signaling pathway is an important regulator of stem cell activity in various tissues, including the central nervous system. It has been implicated neurodevelopmental processes, neuronal differentiation and synaptic plasticity. Research suggests that its expression may be associated with certain epileptogenic lesions, particularly those origin. aim this study was to investigate Notch-1 brain biopsies from cases pharmacoresistant epilepsy. Methods: Here, we used...
Adenomyosis and endometriosis are distinct gynecological disorders characterized by ectopic growth of endometrial tissue. Their etiology remains unclear, but stem cells have been implicated in both. The aim this study was to investigate compare the quantity NOTCH1+ CD117+ adenomyosis lesions. Immunohistochemical staining endometrium biopsies using antibodies against NOTCH1 CD117 performed. spatial distribution stromal positive for these markers were determined compared between Additionally,...
Abstract Background Sperm abnormalities may negatively affect embryo development. Objectives To determine the influence of sperm (morphology, motility, DNA fragmentation) on morphokinetic variables and clinical outcome conventional IVF. Materials methods Participants were 86 couples undergoing in vitro fertilization (IVF). morphology was evaluated according to strict criteria proposed by Kruger/Tygerberg. CASA system applied for motility assessment. fragmentation assessed chromatin structure...
The purpose of this study was to evaluate the efficacy frozen mixed double-embryo transfer (MDET; simultaneous day 3 and 5 embryos) in comparison with blastocyst (BDET; two blastocysts) patients repeated implantation failure (RIF).A total 104 women RIF who underwent MDET (n=48) or BDET (n=56) excellent-quality embryos were included retrospective analysis. All embryo transfers performed natural cycles. main outcome measures rate, clinical pregnancy multiple miscarriage rate. These compared...
Sperm DNA fragmentation (SDF) and sperm morphological defects can negatively affect ART outcomes. Consequently, there is a need for additional semen processing technique that accounts status morphology prior to ICSI. The objective was evaluate the efficacy of an zona pellucida adhesion-based selection obtaining populations with high percentage normal integrity as compared native routine swim-up preparation. Semen samples from 78 normozoospermic men were subjected swim up placed in petri...
The process of embryo implantation is carried out during the receptive stage endometrium in midluteal phase menstrual cycle, known as window (WOI). It has been assumed that WOI not a constant variable all women and determination its displacement crucial importance, especially for patients with recurrent failure (RIF). Furthermore, rare cases it could have different duration position cycle even same woman but periods. Here, we report 37-year-old RIF, who was previously classified idiopathic...
Platelet-rich plasma (PRP) is used for successful regeneration of female reproductive tissues. However, little known about the effect ovarian PRP treatment on oocyte and embryo quality. The objective our study was to assess role autologous reserve number quality oocytes embryos in women with poor response (POR) undergoing in-vitro fertilization cycles. A total 66 POR were treated injection two subsequent menstrual antral follicle counts, serum anti-Mullerian hormone, follicle-stimulating...
The aim of this prospective study was to compare the endometrial microbiome between pregnant and non-pregnant women after frozen embryo transfer (FET) with euploid embryos. Endometrial biopsies were collected from 30 during mid-luteal phase in a natural cycle. FET performed embryos up 3 months biopsy. microbiota composition analysed using 16S rRNA (v4-v5 region) next generation sequencing (NGS). analysis different clinical outcomes biopsy (no pregnancy (n = 14), ultrasound confirmed 16))...
Zona pellucida is one of the main selective barriers for spermatozoa before reaching oocyte. Using native zona in sperm selection prior to Intracytoplasmic Sperm Injection (ICSI) has been proven effective but inconvenient approach In Vitro Fertilisation (IVF) laboratory. The application autologous solubilised zonae pellucidae ICSI not studied yet.
Drawing inspiration from natural selection and genetic principles, algorithms serve as heuristic global optimization tools.They excel in tackling intricate simulation challenges that conventional methods struggle to solve.While traditionally align more with RNA concepts, this paper introduces a DNA-inspired adaptation of these algorithms.The study validates modification through programmatic implementation, leveraging the openGA opensource library, assesses its efficacy using established...
Abstract The present study aimed to identify, quantify and compare the quantities of very small embryonic-like (VSEL) stem cells in two main fractions fresh umbilical cord blood (UCB). To do this, UCB obtained during caesarean section underwent gradient centrifugation. Nucleated isolated from – mononuclear cell layer above gradient, red pellet below were immunofluorescently labelled identify VSELs (CD45 − /CD133 + /SSEA4 ). These then analysed via multiparameter sorting on a flow cytometer...
Herein, we report the first case of mild malformation cortical development with oligodendroglial hyperplasia and epilepsy (MOGHE) in Bulgaria. It is a newly recognised clinico-pathological entity medically intractable focal paediatric patients. The patient interest 9-year-old boy who has been suffering from refractory since age three. Positron emission tomography revealed consistent hypometabolism maximum orbitofrontal fronto-opercular cortex, as well adjacent anterior insula temporal...
The objective of this study was to compare the motility, morphology, and levels DNA fragmentation spermatozoa subjected conventional swim-up or cumulus matrix (CM) sperm selection. Semen samples were collected from 60 normozoospermic men at a private hospital between December 2021 March 2022. After liquefaction, semen separated into two portions – one part preparation, remaining CM obtained by mechanical isolation healthy donor oocytes. analysis evaluation performed according WHO 6th Edition...
Abstract Study question Are there any quantitative associations between endometrial macrophages populations and microorganisms’ diversity? Summary answer The quantities spatial distribution of correlated significantly with several microbiome diversity indices. What is known already Numerous studies have linked the relative abundance Lactobacilli a receptive endometrium, while presence multiple other bacterial species has been implicated in implantation failure. Another determinant...