Paper-based analytical devices are the subject of growing interest for development low-cost point-of-care diagnostics, environmental monitoring technologies, and research tools limited-resource settings. However, there limited chemistries available conjugation biomolecules to cellulose use in biomedical applications. Herein, divinyl sulfone (DVS) chemistry was demonstrated immobilize small molecules, proteins, DNA covalently onto hydroxyl groups membranes through nucleophilic addition....

Photoacoustic imaging has emerged as a highly promising tool to visualize molecular events with deep tissue penetration. Like most other modalities, however, image contrast under in vivo conditions is far from optimal due background signals tissue. Using iron oxide–gold core–shell nanoparticles, we have previously demonstrated the concept of magnetomotive photoacoustic (mmPA) imaging, which capable dramatically reducing influence and producing high-contrast images. Here, report two...

Silicon photonic microring resonators have established their potential for label-free and low-cost biosensing applications. However, the long-term performance of this optical sensing platform requires robust surface modification biofunctionalization. Herein, we demonstrate a conjugation strategy based on an organophosphonate coating vinyl sulfone linker to biofunctionalize silicon biomolecular sensing. To validate method, series glycans, including carbohydrates glycoconjugates, were...

Microarrays and biosensors owe their functionality to our ability display surface-bound biomolecules with retained biological function. Versatile, stable, facile methods for the immobilization of bioactive compounds on surfaces have expanded application high-throughput "omics"-scale screening molecular interactions by nonexpert laboratories. Herein, we demonstrate potential simplified chemistries fabricate a glycan microarray, utilizing divinyl sulfone (DVS)-modified covalent natural...

With the rapid development of distributed renewables, DC transmission, and active loads, increasingly more power electronics are will be introduced into distribution grids. This has led to an increasing need for a new synchronized measurement system that covers different scenarios applications improve stability reliability power-electronics-enabled In this paper, full-view (SYMS) controls, waveforms grids is established, composed types devices (SMDs) data center. A general design method...

CDKN2B antisense RNA 1 (CDKN2B-AS1), a long noncoding RNA, was reported to play crucial roles in the progression of multiple cancers. However, functional and regulatory mechanism CDKN2B-AS1 human laryngeal squamous cell cancer (LSCC) remain unclear. The goals this study were investigate biological underlying mechanisms LSCC.The expressions CDKN2B-AS1, miR-497 cyclin-dependent kinase 6 (CDK6) detected LSCC tissues lines by real-time quantitative PCR (qRT-PCR). effects on proliferation,...

In this study, we employed thiolated peptides of the conformationally constrained, strongly helicogenic α-aminoisobutyric acid (Aib) residue to prepare self-assembled monolayers (SAMs) on gold surfaces. Electrochemistry and infrared reflection absorption spectroscopy support formation very well packed Aib-peptide SAMs. The immobilized retain their helical structure, resulting SAMs are stabilized by a network intermolecular H bonds involving NH groups adjacent Au surface. Binary containing...

Purpose: To investigate the role of Nrf2/HO-1 signaling pathway in angiogenesis and whether dextran sulfate (DS) could suppress by inhibiting gastric cancer.Methods: In vitro; Western blot analyzed expression Nrf2 cell lines.Tube formation assay observed effect gradient concentration DS on angiogenic potential HGC-27 cells.Immunofluorescence,western qPCR effects Nrf2, HO-1 VEGF under hypoxia time.Immunofluorescence,western blot,qPCR tube up-regulating or down-regulating cells.In vivo:...

A multitime programmable memory cell with improved MOS capacitor is proposed in this paper. The has p-type junction near the channel, which prevents from deep depletion, and helps to improve cell's program/erase efficiency stability. test chip fabricated using a 0.13-μm standard CMOS process without any extra masks or modification. experimental results show that achieves much faster more stable speed than do cells conventional capacitor. Furthermore, endurance characteristics of up 10-k...

The proapoptotic potential of tumor necrosis factor-alpha (TNF-α) has been demonstrated for various cell types, whereas nuclear factor-kappaB (NF-ĸB) is known to support the transcription prosurvival genes. In present study, investigation normal human melanocytes revealed induction apoptosis after TNF-α treatment (100 U/ml) in only 3 out 11 cultures analyzed, 8 remained largely resistant. sensitive cultures, NF-ĸB binding activity was found increased treatment; apoptosis-resistant cells were...

Limited understanding of mitochondria disorders that induced by nanoparticles is a stumbling block for anti-cancer drug delivery targeting strategy. In present study, C6 glioma cells were exposed to aminated and alkylated SiO₂ mitochondrion disordering cell metabolism study. Collective results showed tend trigger the cell-repair mechanism in cancer while could cause irreversible damages on cells, although both types particles proved damage mitochondrion. The underlying show proton-stuck...

Abstract Echelon use batteries from electric vehicles will bring not only the cost reduction of energy storage but also social benefits circular using resource, conservation and emission reduction. It is an important echelon orientation that retired are rebuilt into distributed systems. The article introduces 8 cases systems containing batteries, whose application scenarios include load shifting, renewable storage, frequency modulation power system, capacity charge management. In summary,...

Abstract In-cell immunoassays have become a valuable tool for protein expression analysis complementary to established assay formats. However, comprehensive molecular characterization of individual specimens has proven challenging and impractical due to, in part, singleplex nature reporter enzymes technical complexity alternative Herein, we describe simple robust methodology multiplexed profiling on the same intact specimen, employing well-characterized enzyme alkaline phosphatase accurate...

Foreign body (FB) aspiration in adults is occasionally encountered. The and impaction of a coin the lower respiratory tract an unusual accident. This report presents 2 rare adult cases FB with larynx trachea, respectively.Two patients presented to emergency department separately. Both had similar history inadvertent ingestion 5-jiao that was accidentally enveloped dumplings. immediately followed by violent cough.The diagnosis foreign confirmed chest x-ray. In first patient, computed...

A team of scientists led by Professor DW Pang at Wuhan University have developed a new class fluorescence probes based on bacterial cells. These microbial factories manufacture semiconductor nanocrystals inside and display protein molecules cell surface, transforming Staphylococcus aureus (S. aureus) cells into highly fluorescent cellular beacons that can be easily adapted for detection diverse biological targets.

Abstract not Available.

Benign tracheal stenosis mainly appears due to tracheotomy, tuberculosis, trauma, benign tumor, or ventilation. With the increase in number of tracheotomies and prolongation life span patients after incision, long-term complications tracheotomy gradually increase, among which intratracheal granulation hyperplasia is a more serious complication. The present case describes 59-year-old male with tissue induced by tracheotomy. He underwent resection remove he remained well follow-up. Even though...

Chronic inflammation can stimulate the formation and progression of atherosclerotic plaques increase vulnerability plaques. However, there are few studies on changes carotid inflammatory during treatment. Our study attempted to investigate use superparamagnetic iron oxide nanoparticle (SPION) ultrasound imaging detect expression vascular cell adhesion molecule-1 (VCAM-1) in patients with analyze effects SPION plaque visualization effect. microbubble contrast agents have good both vivo vitro....