A5025715797- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Gastric Cancer Management and Outcomes
- Cancer-related gene regulation
- RNA modifications and cancer
- MicroRNA in disease regulation
- Genomics, phytochemicals, and oxidative stress
- Phytochemical Studies and Bioactivities
- RNA regulation and disease
- Macrophage Migration Inhibitory Factor
- Hernia repair and management
- Cell Adhesion Molecules Research
- TGF-β signaling in diseases
- Phagocytosis and Immune Regulation
- Myofascial pain diagnosis and treatment
- Computer Graphics and Visualization Techniques
- Angiogenesis and VEGF in Cancer
- Cancer Mechanisms and Therapy
- Intraperitoneal and Appendiceal Malignancies
- ATP Synthase and ATPases Research
- Microbial metabolism and enzyme function
- Ultrasound and Hyperthermia Applications
- Metastasis and carcinoma case studies
The Fourth People's Hospital of Ningxia Hui Autonomous Region
2018-2024
Ningxia Hui Autonomous Region Peoples Hospital
2016-2024
Sichuan International Studies University
2021-2022
China Southern Power Grid (China)
2022
Ningxia Medical University
2011-2022
Ningxia University
2003
Kyoto Prefectural University of Medicine
2001
The peritoneum, especially the omentum, is a common site for gastric cancer (GC) metastasis. Our aim was to expound role and mechanisms of Piezo1 on GC omentum A series functional assays were performed examine cell proliferation, clone formation, apoptosis, Ca2+ influx, mitochondrial membrane potential (MMP) migration after overexpression or knockdown Piezo1. peritoneal implantation metastasis model conducted. After infection by si-Piezo1, number growth tumours observed in abdominal cavity....
Gastric cancer (GC) is one of the most fatal digestive tumors worldwide. Abnormal activation or accumulation nuclear factor-erythroid 2-related factor 2/heme oxygenase 1 (Nrf2/HO-1) axis a malignant event in numerous solid tumors. However, its involvement angiogenesis GC remains unknown. This study investigated role Nrf2/HO-1 GC.The expression Nrf2, HO-1, and vascular endothelial growth (VEGF) BGC-823 cells under hypoxia was analyzed using immunocytochemistry, immunofluorescence, Western...
Increasing evidences suggest that mitochondrial calcium uniporter (MCU), a selective channel responsible for Ca2+ uptake, is involved in the progression of several cancers. In this study, we aimed to observe clinical implications and biological functions MCU gastric cancer.The expression 90 pairs cancer tissues adjacent normal was examined using immunohistochemistry correlation between features analyzed. After construction stable knockdown or overexpression cells, membrane potential (MMP),...
Objective: Peritoneal metastasis frequently occurs in advanced gastric cancer, which is typically not eligible for radical surgery. Here, this study observed the function and regulatory mechanism of ADAR1 peritoneal cancer. Methods: ADAR1, CALR β-catenin proteins were detected normal mucosa, primary metastatic lymph node omentum tissues by immunohistochemistry, western blot, immunofluorescence. After silencing siADAR1, effect on cancer nude mouse models with as well HGC-27 AGS cells. Result:...
The aim of the present study was to investigate effects dextran sulphate (DS) on HIF-1α and integrin β1 (ITGβ1) expression in human gastric cancer cells, correlation between ITGβ1 influence DS peritoneal metastasis cells. In vitro experiments, BGC-823 cells experimental control groups were administered PBS, respectively, exposed hypoxic conditions for different periods. Immunocytochemistry, western blot RT-PCR analyses used evaluate levels. vivo an animal model established by injecting into...
To explore the effect of dextran sulfate (DS) on angiogenesis, invasion, and migration gastric cancer cells by interfering with polarization M2-type macrophages.The infiltration macrophages microvascular density in paracancerous tissues were analyzed using immunohistochemistry immunofluorescence. The effects DS angiogenesis metastatic tumors investigated nude mice intraperitoneal metastasis model western blot. differentiation macrophages, immunocytochemistry, blot, ELISA, transwell assay...
Purpose: To investigate the role of Nrf2/HO-1 signaling pathway in angiogenesis and whether dextran sulfate (DS) could suppress by inhibiting gastric cancer.Methods: In vitro; Western blot analyzed expression Nrf2 cell lines.Tube formation assay observed effect gradient concentration DS on angiogenic potential HGC-27 cells.Immunofluorescence,western qPCR effects Nrf2, HO-1 VEGF under hypoxia time.Immunofluorescence,western blot,qPCR tube up-regulating or down-regulating cells.In vivo:...
Gastric cancer (GC) is a malignant tumor with high global mortality rate that currently difficult to treat. Dextran sulfate (DS), safe anti-tumor agent, can effectively inhibit the biological behavior of gastric cancer; however, its mechanism action not fully understood. Therefore, this study aimed at elucidate potential mechanisms action.
The peritoneal implant metastasis is one of the main pathway and cause for high mortality gastric cancer metastasis.Researchs show that epithelial-mesenchymal transition (EMT) playing essential role in modulating metastasis, expression hypoxia inducible factor-1α (HIF-1α) can promote EMT tumor cells.This research aims to explore influence mechanism Dextran Sulfate (DS) affecting human cancer.In present study, we found DS enter into cytoplasm function it.Inhibition HIF-1α or significantly...
The objective of the present study was to observe influence dextran sulfate (DS) on proliferation, invasion and migration AGS, BGC-23, GES-1, MGC-803 SGC-7901 cells. Additionally, possible inhibition mechanism DS BGC-823 cells epithelial-mesenchymal transition (EMT) explored. in control experimental group were treated with PBS respectively. effect these five types investigated using Transwell assays. Immunocytochemistry, western blotting reverse transcription-polymerase chain reaction...
In the present study, effect of dextran sulfate (DS) on metastasis and invasion human gastric cancer cells its key underlying mechanism were investigated. The levels hypoxia‑inducible factor 1α (HIF‑1α), transforming growth β (TGF‑β) lysyl oxidase (LOX) expression evaluated in peritumoral tissues by immunohistochemical analysis. Cell proliferation apoptosis also examined using Counting Kit‑8 assay flow cytometry. DS migration BGC‑823 was assessed a Transwell assay. divided into control...
Gastric cancer(GC)is one of the deadliest digestive tract tumors worldwide，existing studies suggest that dysregulated expression microRNAs (miRNAs) plays an important role in pathogenesis and progression GC. This study aimed to investigate expression, biological function, downstream mechanism miR-34c-5p GC, provide new targets for gastric cancer diagnosis treatment.The GC tissues cell lines was examined by RT-qPCR. Cell wound healing, transwell cloning assays were used detect effect on...
Treatment failure of surgically treated gastric cancer is attributed to the spread cells into abdominal cavity and lymphatic or hematogenic canals. In present study, local injection mitomycin C bound activated carbon (M-CH) combined with i.p. hyperthermic hypo-osmolar infusion (IPHHOI) was intraoperatively administered prevent lymph node recurrence peritoneal cancer. Between April 1998 August 1999, 79 patients advanced were allocated randomly two groups. Forty underwent M-CH plus IPHHOI...
The present study investigated the inhibitory effects of dextran sulphate (DS) on peritoneal metastasis gastric cancer by observing adhesion and implantation human cells in omenta nude mice. DS or PBS was added to culture medium MKN1 cells. dishes, morphological changes fixed living were observed using fluorescence staining confocal microscopy. In addition, expression integrin β1 measured immunohistochemistry reverse transcription-polymerase chain reaction (RT-PCR). Gastric BGC-823...
In the present study we investigated enhancement of cytotoxicity peritoneal macrophages induced by OK-432. Rats received an i.p. injection OK-432 at doses 0.1, 0.5 or 1.0 KE/rat. Two days later, rats were sacrificed and isolated. Then number was counted, analyzed for their lactic dehydrogenase (LDH) activity, acid phosphatase (ACP) phagocytic secretion nitric oxide (NO) cytotoxicity. The macrophages, activity LDH ACP, NO secretion, increased with increasing results suggested that enhanced...
With the increasingly serious situation of population aging in China, impact cannot be ignored. This study analyzes effect on attraction FDI inflows China through construction an empirical model. The results show that there is a significant “inverted-U” relationship between and inflows, negatively affects foreign investment after crossing inflection point. Based findings, this proposes policy recommendations based quantity quality labor force, such as improving gradual delayed retirement,...
Gastric cancer (GC) is one of the most common gastrointestinal malignancies. According to reports, enhancer zeste homolog 2 (EZH2) exhibits carcinogenic function in a variety cancers. Therefore, EZH2 may be potential therapeutic target for treatment human cancer. Macromolecular Dextran Sulfate (DS) has been displayed play critical role tumor inhibition. However, molecular mechanism by which DS mediates this effect unclear.In study, we explored effects on proliferation and apoptosis gastric...
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