Abstract Soybean accounts for more than half of the global production oilseed and a quarter protein used globally human food animal feed. domestication involved parallel increases in seed size oil content, concomitant decrease content. However, science has not yet discovered whether these effects were due to selective pressure on single gene or multiple genes. Here, re-sequencing data from >800 genotypes revealed strong selection during soybean GmSWEET10a. The GmSWEET10a conferred...

To understand the gene expression networks controlling soybean seed set and size, transcriptome analyses were performed in three early developmental stages, using two genotypes with contrasting size. The two-dimensional data provides a comprehensive systems-level view on dynamic underpinning subsequent development. Using pairwise comparisons weighted coexpression network analyses, we identified modules of coexpressed genes hub for each module. Of particular importance are discoveries...

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To maintain a stable cytosol phosphate (Pi) concentration, plant cells store Pi in their vacuoles. When the concentration decreases, is exported from vacuole into cytosol. This export mediated by transporters on tonoplast. In this study, we demonstrate that SYG1, PHO81, and XPR1 (SPX)-Major Facility Superfamily (MFS) proteins have similar structure with yeast (Saccharomyces cerevisiae) low-affinity Phosphatase87 (PHO87), PHO90, PHO91. OsSPX-MFS1, OsSPX-MFS2, OsSPX-MFS3 all localized...

Iron (Fe) is an essential micronutrient that participates in various biological processes important for plant growth. Ethylene production induced by Fe deficiency plays roles tolerance to stress deficiency. However, the activation and regulatory mechanisms of 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) genes this response are not clear. In study, we demonstrated increased abundance ACS2, ACS6, ACS7, ACS11 transcripts both leaf root as well ACS8 ACS9 root. Furthermore, investigated...

Plants have evolved two strategies to acquire ferrous (Strategy I) or ferric II) iron from soil. The iron-related bHLH transcription factor 2 (IRO2) has been identified as a key regulator of acquisition in rice. However, its mode action, subcellular localisation and binding partners are not clearly defined. Using RNA-seq analyses, we novel bHLH-type factor, OsbHLH156. function OsbHLH156 Fe homeostasis was analysed by characterisation the phenotypes, elemental content, transcriptome,...

Phosphate overaccumulator2 (PHO2) encodes a ubiquitin-conjugating E2 enzyme that is major negative regulator of the inorganic phosphate (Pi)-starvation response-signaling pathway. A yeast two-hybrid (Y2H) screen in rice (Oryza sativa; Os) using OsPHO2 as bait revealed an interaction between and two h-type thioredoxins, OsTrxh1 OsTrxh4. These interactions were confirmed vivo bimolecular fluorescence complementation (BiFC) OsTrxh1/h4 protoplasts by vitro pull-down assays with 6His-tagged...

Abstract Although CRISPR-Cas9 technology has been rapidly applied in soybean genetic improvement, it is difficult to achieve the targeted editing of specific loci complex genome due limitations classical protospacer adjacent motif (PAM). Here, we developed a PAM-less system mediated by SpRY soybean. By performing representative agronomic trait targets and evaluating results, demonstrate that protein can efficient mutagenesis at relaxed PAM sites Furthermore, SpRY-based cytosine base editor...

Background: Transforming growth factor β1 (TGFβ1) was one of the main factors for accelerating atrial fibrosis and has been reported with significantly higher level in plasma patients essential hypertension (EH), especially those target organ damage. The contribution TGFβ1 pathogenesis fibrillation (AF) EH remains unknown. Methods: 75 documented AF were divided into paroxysmal group (EH+pAF, n = 44) or chronic (EH+cAF, 31), 37 sinus rhythm (SR) assigned EH+SR group. All data including...

Soybean (Glycine max) is an important crop providing oil and protein for both human animal consumption. Knowing which biological processes take place in specific tissues a temporal manner will enable directed breeding or synthetic approaches to improve seed quantity quality. We analyzed genome-wide transcriptome dataset from embryo, endosperm, endothelium, epidermis, hilum, outer inner integument suspensor at the global, heart cotyledon stages of soybean development. The tissue specificity...

BACKGROUND:Transforming growth factor (TGF)-β1 is involved in the pathogenesis of coronary artery disease (CAD), but mechanism its action remains unclear. Our study aimed to investigate role TGF-β1 CAD and explore possible mechanisms. MATERIAL AND METHODS:A total 60 patients 54 healthy people were included this study. Blood samples drawn from each participant prepare serum. ELISA was utilized measure serum level TGF-β1. expression vector, siRNA, TIMP-1 siRNA transfected into human primary...

The new gene-editing technology CRISPR/Cas system has been widely used for genome engineering in various organisms. Since the a certain possibility of low efficiency and whole plant transformation soybean is time-consuming laborious, it important to evaluate editing designed CRISPR constructs before stable process starts. Here, we provide modified protocol generating transgenic hairy roots assess guide RNA (gRNA) sequences within 14 days. cost- space-effective was first tested harboring GUS...

CHF3 is a strong greenhouse gas, and its potential effect 14,800 times that of CO2, so recycling reusing positively contributes to environmental sustainability. Therefore, purification recovery process development based on the adsorption has become an urgent requirement. In this paper, ultramicroporous fluorine-containing pillared metal–organic framework (MOF), SIFSIX-3-Ni, was applied separation CHF3/N2. The properties SIFSIX-3-Ni for CHF3/N2 were confirmed by single-component isotherms...

Iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn) are essential micronutrients that necessary for plant growth development, but can be toxic at supra-optimal levels. Plants have evolved a complex homeostasis network includes uptake, transport, storage of these metals. It was shown the transcription factor (TF) OsbHLH156-OsIRO2 is activated under Fe deficient conditions acts as central regulator on Strategy II acquisition. In this study, role TF Mn, Cu, Zn uptake evaluated. While...

Hydrofluorocarbons (HFCs) have important applications in different industries; however, they are environmentally unfriendly due to their high global warming potential (GWP). Hence, reclamation of used hydrofluorocarbons via energy-efficient adsorption-based separation will greatly contribute reducing impact on the environment. In particular, azeotropic refrigerants remains challenging, such as typical mixtures CH2F2 (HFC-23) and CHF3 (HFC-32), a lack adsorptive mechanisms. Metal–organic...

Iron (Fe) deficiency directly affects crop growth and development, ultimately resulting in reduced yield quality. Recently, long non-coding RNAs (lncRNAs) have been demonstrated to play critical regulatory roles a multitude of pathways across numerous species. However, systematic screening lncRNAs responding Fe their mechanism plants has not reported. In this work, 171 differently expressed (DE-lncRNAs) were identified based on analysis strand-specific RNA-seq data from rice shoots roots...

Left atrial appendage occlusion (LAAO) is usually performed via the guidance of procedural transesophageal echocardiography (TEE) companied by general anesthesia (GA).To investigate feasibility and safety LAAO guided fluoroscopy only.The patients eligible for were enrolled into current study received implantation either Watchman device or LAmbre device. The procedure was carried out with only no GA; position, shape, leakage assessed contrast angiography. TEE after 3-month follow-up to...

Magnesium hydride (MgH2) is considered as one of the most potential hydrogen storage materials. However, limited by hydrolysis kinetics, controllability and sustainability MgH2 are poor. In view this, a porous filter element employed optimized to improve performance. The effects different structural parameters, operating physical parameters on performance were investigated. controllable continuous was achieved using with an appropriate overflow weir. research in this paper can provide...

Soybean sclerotinia stem rot (SSR) is a disease caused by Sclerotinia sclerotiorum that causes incalculable losses in soybean yield each year. Considering the lack of effective resistance resources and elusive mechanisms, we are urged to develop genes explore their molecular mechanisms. Here, found loss GmSWEET15 enhanced S. sclerotiorum, explored mechanisms which gmsweet15 mutant exhibit comparing transcriptome. At early stage inoculation, wild type (WT) showed moderate defense response,...