A5103089842- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Insect Resistance and Genetics
- RNA Interference and Gene Delivery
- Microbial Inactivation Methods
- Transgenic Plants and Applications
- Chronic Lymphocytic Leukemia Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Insect-Plant Interactions and Control
- Microfluidic and Bio-sensing Technologies
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
- Viral Infectious Diseases and Gene Expression in Insects
- Insect Pest Control Strategies
- Antimicrobial Peptides and Activities
- Pressure Ulcer Prevention and Management
- Air Quality and Health Impacts
- Advanced Steganography and Watermarking Techniques
- Renal and related cancers
- Medicinal Plant Pharmacodynamics Research
- Hemoglobinopathies and Related Disorders
- Molecular Communication and Nanonetworks
Zhejiang Chinese Medical University
2023
China Agricultural University
2020-2022
MaxCyte (United States)
2011-2021
Taihe Hospital
2020
Hubei University of Medicine
2020
Henan Agricultural University
2019
Xiangya Hospital Central South University
2015
Central South University
2015
Beijing Institute of Graphic Communication
2012
Shanghai Medical College of Fudan University
1994
CRISPR-mediated gene repair of hematopoietic stem cells from patients with X-linked chronic granulomatous disease resulted in functional human leukocytes mice after transplantation.
How horizontal gene transfer (HGT) has contributed to the evolution of animals and plants remains a major puzzle. Despite recent progress, defining overall scale pattern HGT events in land been largely elusive. In this study, we performed systematic analyses for acquired genes different plant groups throughout evolution. We found that relatively occurred charophytes all groups, but their frequency declined rapidly seed plants. Two episodes evolution, corresponding early streptophytes origin...
For more than a decade, investigators have pursued methods to genetically engineer natural killer (NK) cells for use in clinical therapy against cancer. Despite considerable advances viral transduction of hematopoietic stem and T cells, efficiencies NK remained disappointingly low. Here, we show that can be reprogramed efficiently using cGMP-compliant mRNA electroporation method induces rapid reproducible transgene expression nearly all transfected without negatively influencing their...
CD19-targeted chimeric antigen receptor (CAR) engineered T/natural killer (NK)-cell therapies can result in durable clinical responses B-cell malignancies. However, CAR-based immunotherapies have been much less successful solid cancers, part due to "on-target off-tumor" toxicity related expression of target tumor antigens on normal tissue. Based preliminary observations safety and activity proof-of-concept trials, antigen-specific messenger RNA (mRNA) CAR transfection into selected,...
The evolution of resistance to insecticides is well known be closely associated with the overexpression detoxifying enzymes. Although role glutathione S -transferase (GST) genes in insecticide has been widely reported, underlying regulatory mechanisms are poorly understood. Here, one GST gene ( GSTu1 ) and its antisense transcript (lnc- -AS) were identified cloned, both them upregulated several chlorantraniliprole-resistant Plutella xylostella populations. was confirmed involved...
Abstract Kidney renal clear cell carcinoma (KIRC) is the most general subtype of carcinoma, which composes about 1/20 adult malignancies. The anomaly long noncoding RNAs (lncRNAs) expression proved to mediate cancer progression various types. function and mediation mechanism MSC‐AS1 has rarely been detected in KIRC before. This study started with on function. In this study, was dramatically upregulated correlated dismal prognosis patients. Knockdown would suppress proliferative migratory...
The diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera: Plutellidae) is an insect pest found around the world that feeds on cruciferous crops. DBM has become resistant to most insecticides in current use field. Broflanilide a novel meta-diamide insecticide binds new site γ-aminobutyric acid receptor and very efficiently protects against pests order Lepidoptera, including DBM. In this study, resistance of laboratory-bred susceptible strain broflanilide fitness costs posed by were...
Sickle cell disease (SCD) is caused by a 20A > T mutation in the β-globin gene. Genome-editing technologies have potential to correct SCD hematopoietic stem cells (HSCs), producing adult hemoglobin while simultaneously eliminating sickle hemoglobin. Here, we developed high-efficiency viral vector-free non-footprint gene correction CD34+ with electroporation deliver mutation-targeting guide RNA, Cas9 endonuclease, and 100-mer single-strand donor DNA encoding intact sequence, achieving...
Electroporation is widely used to transfect and load cells with various molecules. Traditional electroporation using a static mode typically restricted volumes less than 1 mL, which limits its use in clinical industrial bioprocessing applications. Here we report efficient, large volume transfection results by scalable-volume system. Suspended (Jurkat) adherent (10T1/2 Huh-7) were tested. A macromolecule, FITC-conjugated dextran (MW=500 kD) was measure cell uptake, while plasmid carrying the...
Abstract BACKGROUND Carboxylesterases (CarEs) are associated with detoxification of xenobiotics, including insecticides, in organism bodies. Overexpression CarE genes is considered to have an important role insecticide resistance insects, however its involvement multi‐insecticide has rarely been reported. This study aimed assess the function a gene ( PxαE8 ) five insecticides Plutella xylostella . RESULTS Relative expression three multi‐insecticide‐resistant populations, GD‐2017, GD‐2019 and...
Helicoverpa armigera is a universal pest around the world that has been extensively used as model organism for agricultural pests. Calcineurin (CAN) an important Ca2+-dependent phosphatase participated in various biological pathways. Here, we revealed CAN inhibition significantly arrested H. larval development by reducing larvae weight, prolonging time and pupate rates. Furthermore, serves immune activator regulates antimicrobial peptide (AMP; cecropin D, attacin, gloverin) expression...
Considerable efforts have been devoted to exploring the breast cancer mutational landscape understand its genetic complexity. However, no studies yet comprehensively elucidated molecular characterization of tumors in Chinese women. This study aimed determine potential clinical utility peripheral blood assessment for circulating tumor-derived DNA (ctDNA) and characterize female population's spectrum. We used Omi-Seq create profiles 273 patients enrolled at The First Affiliated Hospital...
Electroporation of dendritic cells (DCs) with tumor lysate elicited greater antitumor responses in vitro and vivo, using less than standard coincubation. Electroloaded DCs had normal surface marker expression matured into competent antigen-presenting cells. In a renal carcinoma (RENCA) model, mice were pretreated lysate-loaded before challenge. Mice that received electroloaded RENCA significantly smaller tumors (9+/-6 mm2) given coincubated the same (23+/-5 mm2). To evaluate metastatic...