In vitro digestion products of proteins were compared among beef, pork, chicken, and fish. Gastric jejunal contents from the rats fed these meat also compared. Cooked fish homogenized incubated with pepsin alone or followed by trypsin. The molecular weights less than 3000 Da identified MALDI-TOF-MS nano-LC-MS/MS. obtained four for 7 days analyzed. After digestion, beef samples had a greater number fragments in similarity chicken samples, but digestibility was greatest (p < 0.05) pork...

The main purpose of this study was to investigate the effects oxidation in vitro on biochemical properties myofibrillar protein isolates (MPIs) from beef muscles. MPIs were incubated at 4 °C for 24 h with hydroxyl-radical-generating systems consisting 0.01 mM FeCl3 and 0.1 ascorbic acid plus 0, 0.2, 1, 5, 10, 20 hydrogen peroxide. results showed that caused drastically structural changes bovine MPIs. carbonyl content, surface hydrophobicity, particle diameter significantly increased, while...

The influence of xanthan gum (XG) and psyllium husk powder (PHP) at different ratios on the emulsification oxidative stability myofibrillar protein (MP) emulsions were studied under low-salt conditions. results showed that, in comparison to added with XG or PHP alone control, MP supplemented mixture manifested higher apparent viscosity Zeta potential, while having lower oil droplet size. Therefore, containing mixtures best creaming stability. Microstructure images further that droplets...

1. An experiment was conducted to evaluate the effects of dietary glutamine (Gln, 0 and 5 g/kg) gamma-aminobutyric acid (GABA, 100 mg/kg) on raw breast meat colour, pH, composition water-holding characteristic broilers under cyclic heat stress (HS). 2. A total 360 21-d-old Arbor Acres male chicks were randomly assigned treatment groups (6 replicates 12 birds per cage). The positive control (PC) kept in a thermoneutral chamber (22–24°C) fed basal diet. other 4 HS (30–34°C) for 9 h (from 09:00...

Meat-diet-induced changes in gut microbiota are often accompanied with the development of various metabolic and inflammatory disorders. The exact biochemical mechanism underlying these effects is not well elucidated. This study aims to evaluate how meat proteins high-fat diets affect tryptophan metabolism rats. high-chicken-protein (HFHCH) or high-pork-protein (HFHP) increased levels skatole indole cecal colonic contents, feces, subcutaneous adipose tissue. HFHCH HFHP also abundance...

High-fat diets have been associated with overweight/obesity and increased mortality in middle-aged populations. However, it is still unclear how gut microbiota populations responds to dietary fats at a normal dose. In this study, we explored structure rats (aged 12 months) after feeding 4% (w/w) soybean oil, lard or fish oil for 3 months, respectively. The results showed that the group was substantially different from those of groups both vitro vivo studies. relative abundances phylum...

Spreading is the key process for ensuring green tea quality. However, effect of blue light intensity conditions on formation aroma and evolution volatile compounds has not been assessed to date. Four samples treated with different (blue intensities) were used investigate spreading treatment changes in composition content compounds. Volatile detected using headspace-solid phase microextraction gas chromatography-mass spectrometry under conditions. Orthogonal partial least squares discriminant...

Abstract This study reports on a comprehensive comparison of the effects soy and meat proteins given at recommended level physiological markers metabolic syndrome hepatic transcriptome. Male rats were fed semi-synthetic diets for 1 wk that differed only regarding protein source, with casein serving as reference. Body weight gain adipose tissue mass significantly reduced by but not proteins. The insulin resistance index was improved to lesser extent Liver triacylglycerol contents both...

The gut microbiota plays an essential role in linking diet to host health. specific of different dietary proteins on the and health is less understood. Here, impact derived from chicken soy barrier C57BL/6 mice investigated.Specific-pathogen-free germ-free are assigned either a chicken- or protein-based for 4 weeks. Compared with chicken-protein-based diet, intake soy-protein-based reduces abundance A. muciniphila number goblet cells, lowers level Muc2 mRNA, decreases thickness mucus layer...

We report on the impact of purified dietary meat proteins from four species plasma insulin, lipid and amino acid (AA) concentrations, hepatic transcriptome (RNA-sequencing).Young rats received semi-synthetic diets for 1 wk that differed only regarding protein source; casein (reference) was replaced by beef, chicken, fish, or pork proteins. Compared to casein, all proteins, except pork, increased total AA concentrations. Pork reduced adipose tissue mass liver triacylglycerol, which...

It has been reported that isolated dietary soy and meat proteins have distinct effects on physiology liver gene expression, but the impact protein expression responses are unknown. Because these may differ from responses, we investigated protein-induced changes in proteome. Rats were fed for 1 week semisynthetic diets differed only regarding source; casein (reference) was fully replaced by soy, chicken, fish, or pork protein. Changes proteome measured iTRAQ labeling LC–ESI–MS/MS. A robust...

Abstract The impact of dietary soybean oil, lard and fish oil on physiological responses in middle age is little studied. In this study, we investigated the changes oxidative stress, inflammatory cytokines, telomere length, age-related gene expression liver middle-aged rats response to above three fat diets. Male Sprague Dawley (12 months old) were fed AIN-93M diets for 3 months, which was equivalently replaced by or oil. As compared diet, intake diet significantly decreased body weight...

Casein and chicken are assessed to contain high quality proteins, which essential for human health. Studies have shown that ingestion of the two dietary proteins resulted in distinct effects on physiology, liver transcriptome gut microbiota. However, its underlying mechanism is not fully understood, particular a crosstalk between microbiota host under specific diet intervention. We fed young rats with casein or protein-based (CHPD) 7 days, characterized cecal composition gene expression....

Abstract Epidemiological studies have shown that excessive consumption of meat may cause memory loss and cognitive decline, but the underlying mechanism is unknown. Here, a high‐chicken‐protein diet not high‐pork was found to markedly negative impact on enteric nervous system hippocampal function. Higher serine, glutamate, proline levels in inhibited absorption capacity amino acids, reduced neurotransmitters, further increased β‐secretase levels. Alzheimer's disease mouse model cell...

Abstract We investigated the impact of six protein diets on oxidation and anti-oxidation status in muscle young rats. Rats were fed for 14 days, including casein (control), proteins isolated from soy, fish, chicken, pork beef. Grx1, Trx1 other oxidative metabolic indices quantified. Compared with diet, soy diet had a similar level, but higher GSH lower SOD activities. The chicken fish groups activities, group showed Grx1 levels than beef highest GSH, as reflected by RT-PCR, Western blotting...

Casein and meat are food sources providing high-quality animal proteins for human consumption. However, little is known concerning potentially different effects of these protein during early stages life. In the present study, casein red (beef pork) were fed to young postweaning rats 14 days based on AIN-93G diet formula. protein-based diets did not differentially affect overall growth performance. they discriminately modulated abundances beneficial bacteria belonging genus Lactobacillus....

This study compared the effects of casein, soy protein (SP), red (RMP), and white meat (WMP) proteins on growth metabolism young rats. Compared to ratio daily feed intake body weight gain rats was not changed by but reduced SP 93.3% ( P < 0.05). Feeding RMP WMP liver total cholesterol (TC) contents 24.3% 17.8%, respectively Only increased plasma HDL-cholesterol concentrations (by 12.7%, 0.05), whereas triacylglycerol, TC, LDL-cholesterol 23.7%, 19.5%, 61.5%, Plasma essential amino acid were...

The impact of meat protein on metabolic regulation is still disputed and may be influenced by level. This study aimed to explore the effects casein, pork, chicken proteins at different levels (40% E vs 20% E) body weight regulation, fat accumulation, serum hormone levels, inflammatory factors/metabolites in rats maintained high-fat (45% fat) diets for 84 d. Increased resulted a significant reduction mass an increase anti-inflammatory cytokine IL-10, independent source. Analysis blood via...

Effects of different levels (1%, 3% and 5%) cherry powder on the physiochemical properties antioxidant activity Jiangsu-type sausages were investigated at 4 °C for 30 days. The results show that sensory evaluation values physicochemical had no significant differences compared to control group when addition was 1%, alcohols, aldehydes esters increased after improved flavor sausages. However, higher concentration (3% exerted adverse influences with control. could better inhibit lipid protein...

The peptide transporter 1 (PEPT1) in the apical membrane of enterocytes is central mechanism for regulating absorption di- and tripeptides. Dietary proteins may affect PEPT1 abundance absorption. present study aimed to characterize changes mRNA protein levels duodenum jejunum young rats after 7-day diet intervention with casein (reference), soy, beef, pork, chicken fish further evaluate impact on epithelial capacity. RT-PCR western blot analyses showed that: (1) level was higher (p < 0.05)...